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Anatomic Characterization of the Ocular Surface Microbiome in Children

The microbiome is important in the evolution of the immune system in children; however, information is lacking regarding the composition of the pediatric ocular microbiome and its surrounding structures. A prospective, cross-sectional study of the ocular microbiome was conducted in children <18 y...

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Autores principales: Cavuoto, Kara M., Galor, Anat, Banerjee, Santanu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723495/
https://www.ncbi.nlm.nih.gov/pubmed/31416201
http://dx.doi.org/10.3390/microorganisms7080259
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author Cavuoto, Kara M.
Galor, Anat
Banerjee, Santanu
author_facet Cavuoto, Kara M.
Galor, Anat
Banerjee, Santanu
author_sort Cavuoto, Kara M.
collection PubMed
description The microbiome is important in the evolution of the immune system in children; however, information is lacking regarding the composition of the pediatric ocular microbiome and its surrounding structures. A prospective, cross-sectional study of the ocular microbiome was conducted in children <18 years old. Samples from the inferior conjunctival fornix of both eyes, eyelid margin, and periocular skin underwent DNA amplification and 16S sequencing using Illumina MiSeq 250. The microbiome was analyzed using Qiime. Statistical analysis was performed using a two-sided Student’s t-test, diversity indices, and principal coordinate analysis. A total of 15 children were enrolled. The ocular surface microbiome was predominantly composed of Proteobacteria, whereas Bacteroidetes dominated the eyelid margin, and Firmicutes dominated the periocular skin. Despite these variations, no statistically significant compositional differences were found with Bray-Curtis analysis. The conjunctiva had the lowest Shannon diversity index with a value of 2.3, which was significantly lower than those of the eyelid margin (3.4, p = 0.01) and the periocular skin (3.5, p = 0.001). However, the evenness of the species using Faith’s phylogenetic diversity index was similar at all sites. Overall, the ocular surface microbiome is dominated by Proteobacteria in children. The niche is similar to the surrounding structures in terms of composition, but has a lower number and relative abundance of species.
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spelling pubmed-67234952019-09-10 Anatomic Characterization of the Ocular Surface Microbiome in Children Cavuoto, Kara M. Galor, Anat Banerjee, Santanu Microorganisms Article The microbiome is important in the evolution of the immune system in children; however, information is lacking regarding the composition of the pediatric ocular microbiome and its surrounding structures. A prospective, cross-sectional study of the ocular microbiome was conducted in children <18 years old. Samples from the inferior conjunctival fornix of both eyes, eyelid margin, and periocular skin underwent DNA amplification and 16S sequencing using Illumina MiSeq 250. The microbiome was analyzed using Qiime. Statistical analysis was performed using a two-sided Student’s t-test, diversity indices, and principal coordinate analysis. A total of 15 children were enrolled. The ocular surface microbiome was predominantly composed of Proteobacteria, whereas Bacteroidetes dominated the eyelid margin, and Firmicutes dominated the periocular skin. Despite these variations, no statistically significant compositional differences were found with Bray-Curtis analysis. The conjunctiva had the lowest Shannon diversity index with a value of 2.3, which was significantly lower than those of the eyelid margin (3.4, p = 0.01) and the periocular skin (3.5, p = 0.001). However, the evenness of the species using Faith’s phylogenetic diversity index was similar at all sites. Overall, the ocular surface microbiome is dominated by Proteobacteria in children. The niche is similar to the surrounding structures in terms of composition, but has a lower number and relative abundance of species. MDPI 2019-08-14 /pmc/articles/PMC6723495/ /pubmed/31416201 http://dx.doi.org/10.3390/microorganisms7080259 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cavuoto, Kara M.
Galor, Anat
Banerjee, Santanu
Anatomic Characterization of the Ocular Surface Microbiome in Children
title Anatomic Characterization of the Ocular Surface Microbiome in Children
title_full Anatomic Characterization of the Ocular Surface Microbiome in Children
title_fullStr Anatomic Characterization of the Ocular Surface Microbiome in Children
title_full_unstemmed Anatomic Characterization of the Ocular Surface Microbiome in Children
title_short Anatomic Characterization of the Ocular Surface Microbiome in Children
title_sort anatomic characterization of the ocular surface microbiome in children
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723495/
https://www.ncbi.nlm.nih.gov/pubmed/31416201
http://dx.doi.org/10.3390/microorganisms7080259
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