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Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels in Africa and Middle East

Dromedary camels are the natural reservoirs of the Middle East respiratory syndrome coronavirus (MERS-CoV). Camels are mostly bred in East African countries then exported into Africa and Middle East for consumption. To understand the distribution of MERS-CoV among camels in North Africa and the Midd...

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Autores principales: Kandeil, Ahmed, Gomaa, Mokhtar, Nageh, Ahmed, Shehata, Mahmoud M., Kayed, Ahmed E., Sabir, Jamal S. M., Abiadh, Awatef, Jrijer, Jamel, Amr, Zuhair, Abi Said, Mounir, Byarugaba, Denis K., Wabwire-Mangen, Fred, Tugume, Titus, Mohamed, Nadira S., Attar, Roba, Hassan, Sabah M., Abdulaziz Linjawi, Sabah, Moatassim, Yassmin, Kutkat, Omnia, Mahmoud, Sara, Bagato, Ola, Abo Shama, Noura M., El-Shesheny, Rabeh, Mostafa, Ahmed, A. P. M. Perera, Ranawaka, K. W. Chu, Daniel, Hassan, Nagla, Elsokary, Basma, Saad, Ahmed, Sobhy, Heba, El Masry, Ihab, P. McKenzie, Pamela, J. Webby, Richard, Peiris, Malik, J. Makonnen, Yilma, A. Ali, Mohamed, Kayali, Ghazi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723520/
https://www.ncbi.nlm.nih.gov/pubmed/31387326
http://dx.doi.org/10.3390/v11080717
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author Kandeil, Ahmed
Gomaa, Mokhtar
Nageh, Ahmed
Shehata, Mahmoud M.
Kayed, Ahmed E.
Sabir, Jamal S. M.
Abiadh, Awatef
Jrijer, Jamel
Amr, Zuhair
Abi Said, Mounir
Byarugaba, Denis K.
Wabwire-Mangen, Fred
Tugume, Titus
Mohamed, Nadira S.
Attar, Roba
Hassan, Sabah M.
Abdulaziz Linjawi, Sabah
Moatassim, Yassmin
Kutkat, Omnia
Mahmoud, Sara
Bagato, Ola
Abo Shama, Noura M.
El-Shesheny, Rabeh
Mostafa, Ahmed
A. P. M. Perera, Ranawaka
K. W. Chu, Daniel
Hassan, Nagla
Elsokary, Basma
Saad, Ahmed
Sobhy, Heba
El Masry, Ihab
P. McKenzie, Pamela
J. Webby, Richard
Peiris, Malik
J. Makonnen, Yilma
A. Ali, Mohamed
Kayali, Ghazi
author_facet Kandeil, Ahmed
Gomaa, Mokhtar
Nageh, Ahmed
Shehata, Mahmoud M.
Kayed, Ahmed E.
Sabir, Jamal S. M.
Abiadh, Awatef
Jrijer, Jamel
Amr, Zuhair
Abi Said, Mounir
Byarugaba, Denis K.
Wabwire-Mangen, Fred
Tugume, Titus
Mohamed, Nadira S.
Attar, Roba
Hassan, Sabah M.
Abdulaziz Linjawi, Sabah
Moatassim, Yassmin
Kutkat, Omnia
Mahmoud, Sara
Bagato, Ola
Abo Shama, Noura M.
El-Shesheny, Rabeh
Mostafa, Ahmed
A. P. M. Perera, Ranawaka
K. W. Chu, Daniel
Hassan, Nagla
Elsokary, Basma
Saad, Ahmed
Sobhy, Heba
El Masry, Ihab
P. McKenzie, Pamela
J. Webby, Richard
Peiris, Malik
J. Makonnen, Yilma
A. Ali, Mohamed
Kayali, Ghazi
author_sort Kandeil, Ahmed
collection PubMed
description Dromedary camels are the natural reservoirs of the Middle East respiratory syndrome coronavirus (MERS-CoV). Camels are mostly bred in East African countries then exported into Africa and Middle East for consumption. To understand the distribution of MERS-CoV among camels in North Africa and the Middle East, we conducted surveillance in Egypt, Senegal, Tunisia, Uganda, Jordan, Saudi Arabia, and Iraq. We also performed longitudinal studies of three camel herds in Egypt and Jordan to elucidate MERS-CoV infection and transmission. Between 2016 and 2018, a total of 4027 nasal swabs and 3267 serum samples were collected from all countries. Real- time PCR revealed that MERS-CoV RNA was detected in nasal swab samples from Egypt, Senegal, Tunisia, and Saudi Arabia. Microneutralization assay showed that antibodies were detected in all countries. Positive PCR samples were partially sequenced, and a phylogenetic tree was built. The tree suggested that all sequences are of clade C and sequences from camels in Egypt formed a separate group from previously published sequences. Longitudinal studies showed high seroprevalence in adult camels. These results indicate the widespread distribution of the virus in camels. A systematic active surveillance and longitudinal studies for MERS-CoV are needed to understand the epidemiology of the disease and dynamics of viral infection.
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spelling pubmed-67235202019-09-10 Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels in Africa and Middle East Kandeil, Ahmed Gomaa, Mokhtar Nageh, Ahmed Shehata, Mahmoud M. Kayed, Ahmed E. Sabir, Jamal S. M. Abiadh, Awatef Jrijer, Jamel Amr, Zuhair Abi Said, Mounir Byarugaba, Denis K. Wabwire-Mangen, Fred Tugume, Titus Mohamed, Nadira S. Attar, Roba Hassan, Sabah M. Abdulaziz Linjawi, Sabah Moatassim, Yassmin Kutkat, Omnia Mahmoud, Sara Bagato, Ola Abo Shama, Noura M. El-Shesheny, Rabeh Mostafa, Ahmed A. P. M. Perera, Ranawaka K. W. Chu, Daniel Hassan, Nagla Elsokary, Basma Saad, Ahmed Sobhy, Heba El Masry, Ihab P. McKenzie, Pamela J. Webby, Richard Peiris, Malik J. Makonnen, Yilma A. Ali, Mohamed Kayali, Ghazi Viruses Article Dromedary camels are the natural reservoirs of the Middle East respiratory syndrome coronavirus (MERS-CoV). Camels are mostly bred in East African countries then exported into Africa and Middle East for consumption. To understand the distribution of MERS-CoV among camels in North Africa and the Middle East, we conducted surveillance in Egypt, Senegal, Tunisia, Uganda, Jordan, Saudi Arabia, and Iraq. We also performed longitudinal studies of three camel herds in Egypt and Jordan to elucidate MERS-CoV infection and transmission. Between 2016 and 2018, a total of 4027 nasal swabs and 3267 serum samples were collected from all countries. Real- time PCR revealed that MERS-CoV RNA was detected in nasal swab samples from Egypt, Senegal, Tunisia, and Saudi Arabia. Microneutralization assay showed that antibodies were detected in all countries. Positive PCR samples were partially sequenced, and a phylogenetic tree was built. The tree suggested that all sequences are of clade C and sequences from camels in Egypt formed a separate group from previously published sequences. Longitudinal studies showed high seroprevalence in adult camels. These results indicate the widespread distribution of the virus in camels. A systematic active surveillance and longitudinal studies for MERS-CoV are needed to understand the epidemiology of the disease and dynamics of viral infection. MDPI 2019-08-05 /pmc/articles/PMC6723520/ /pubmed/31387326 http://dx.doi.org/10.3390/v11080717 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kandeil, Ahmed
Gomaa, Mokhtar
Nageh, Ahmed
Shehata, Mahmoud M.
Kayed, Ahmed E.
Sabir, Jamal S. M.
Abiadh, Awatef
Jrijer, Jamel
Amr, Zuhair
Abi Said, Mounir
Byarugaba, Denis K.
Wabwire-Mangen, Fred
Tugume, Titus
Mohamed, Nadira S.
Attar, Roba
Hassan, Sabah M.
Abdulaziz Linjawi, Sabah
Moatassim, Yassmin
Kutkat, Omnia
Mahmoud, Sara
Bagato, Ola
Abo Shama, Noura M.
El-Shesheny, Rabeh
Mostafa, Ahmed
A. P. M. Perera, Ranawaka
K. W. Chu, Daniel
Hassan, Nagla
Elsokary, Basma
Saad, Ahmed
Sobhy, Heba
El Masry, Ihab
P. McKenzie, Pamela
J. Webby, Richard
Peiris, Malik
J. Makonnen, Yilma
A. Ali, Mohamed
Kayali, Ghazi
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels in Africa and Middle East
title Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels in Africa and Middle East
title_full Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels in Africa and Middle East
title_fullStr Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels in Africa and Middle East
title_full_unstemmed Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels in Africa and Middle East
title_short Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels in Africa and Middle East
title_sort middle east respiratory syndrome coronavirus (mers-cov) in dromedary camels in africa and middle east
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723520/
https://www.ncbi.nlm.nih.gov/pubmed/31387326
http://dx.doi.org/10.3390/v11080717
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