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Hydrodynamics of Intravitreal Injections into Liquid Vitreous Substitutes

Intravitreal injections have become the cornerstone of retinal care and one of the most commonly performed procedures across all medical specialties. The impact of hydrodynamic forces of intravitreal solutions when injected into vitreous or vitreous substitutes has not been well described. While com...

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Autores principales: Henein, Christin, Awwad, Sahar, Ibeanu, Nkiruka, Vlatakis, Stavros, Brocchini, Steve, Tee Khaw, Peng, Bouremel, Yann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723562/
https://www.ncbi.nlm.nih.gov/pubmed/31374925
http://dx.doi.org/10.3390/pharmaceutics11080371
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author Henein, Christin
Awwad, Sahar
Ibeanu, Nkiruka
Vlatakis, Stavros
Brocchini, Steve
Tee Khaw, Peng
Bouremel, Yann
author_facet Henein, Christin
Awwad, Sahar
Ibeanu, Nkiruka
Vlatakis, Stavros
Brocchini, Steve
Tee Khaw, Peng
Bouremel, Yann
author_sort Henein, Christin
collection PubMed
description Intravitreal injections have become the cornerstone of retinal care and one of the most commonly performed procedures across all medical specialties. The impact of hydrodynamic forces of intravitreal solutions when injected into vitreous or vitreous substitutes has not been well described. While computational models do exist, they tend to underestimate the starting surface area of an injected bolus of a drug. Here, we report the dispersion profile of a dye bolus (50 µL) injected into different vitreous substitutes of varying viscosities, surface tensions, and volumetric densities. A novel 3D printed in vitro model of the vitreous cavity of the eye was designed to visualize the dispersion profile of solutions when injected into the following vitreous substitutes—balanced salt solution (BSS), sodium hyaluronate (HA), and silicone oils (SO)—using a 30G needle with a Reynolds number (Re) for injection ranging from approximately 189 to 677. Larger bolus surface areas were associated with faster injection speeds, lower viscosity of vitreous substitutes, and smaller difference in interfacial surface tensions. Boluses exhibited buoyancy when injected into standard S1000. The hydrodynamic properties of liquid vitreous substitutes influence the initial injected bolus dispersion profile and should be taken into account when simulating drug dispersion following intravitreal injection at a preclinical stage of development, to better inform formulations and performance.
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spelling pubmed-67235622019-09-10 Hydrodynamics of Intravitreal Injections into Liquid Vitreous Substitutes Henein, Christin Awwad, Sahar Ibeanu, Nkiruka Vlatakis, Stavros Brocchini, Steve Tee Khaw, Peng Bouremel, Yann Pharmaceutics Article Intravitreal injections have become the cornerstone of retinal care and one of the most commonly performed procedures across all medical specialties. The impact of hydrodynamic forces of intravitreal solutions when injected into vitreous or vitreous substitutes has not been well described. While computational models do exist, they tend to underestimate the starting surface area of an injected bolus of a drug. Here, we report the dispersion profile of a dye bolus (50 µL) injected into different vitreous substitutes of varying viscosities, surface tensions, and volumetric densities. A novel 3D printed in vitro model of the vitreous cavity of the eye was designed to visualize the dispersion profile of solutions when injected into the following vitreous substitutes—balanced salt solution (BSS), sodium hyaluronate (HA), and silicone oils (SO)—using a 30G needle with a Reynolds number (Re) for injection ranging from approximately 189 to 677. Larger bolus surface areas were associated with faster injection speeds, lower viscosity of vitreous substitutes, and smaller difference in interfacial surface tensions. Boluses exhibited buoyancy when injected into standard S1000. The hydrodynamic properties of liquid vitreous substitutes influence the initial injected bolus dispersion profile and should be taken into account when simulating drug dispersion following intravitreal injection at a preclinical stage of development, to better inform formulations and performance. MDPI 2019-08-01 /pmc/articles/PMC6723562/ /pubmed/31374925 http://dx.doi.org/10.3390/pharmaceutics11080371 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Henein, Christin
Awwad, Sahar
Ibeanu, Nkiruka
Vlatakis, Stavros
Brocchini, Steve
Tee Khaw, Peng
Bouremel, Yann
Hydrodynamics of Intravitreal Injections into Liquid Vitreous Substitutes
title Hydrodynamics of Intravitreal Injections into Liquid Vitreous Substitutes
title_full Hydrodynamics of Intravitreal Injections into Liquid Vitreous Substitutes
title_fullStr Hydrodynamics of Intravitreal Injections into Liquid Vitreous Substitutes
title_full_unstemmed Hydrodynamics of Intravitreal Injections into Liquid Vitreous Substitutes
title_short Hydrodynamics of Intravitreal Injections into Liquid Vitreous Substitutes
title_sort hydrodynamics of intravitreal injections into liquid vitreous substitutes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723562/
https://www.ncbi.nlm.nih.gov/pubmed/31374925
http://dx.doi.org/10.3390/pharmaceutics11080371
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