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Characterization of the First Conotoxin from Conus ateralbus, a Vermivorous Cone Snail from the Cabo Verde Archipelago

Conus ateralbus is a cone snail endemic to the west side of the island of Sal, in the Cabo Verde Archipelago off West Africa. We describe the isolation and characterization of the first bioactive peptide from the venom of this species. This 30AA venom peptide is named conotoxin AtVIA (δ-conotoxin-li...

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Autores principales: Neves, Jorge L. B., Imperial, Julita S., Morgenstern, David, Ueberheide, Beatrix, Gajewiak, Joanna, Antunes, Agostinho, Robinson, Samuel D., Espino, Samuel, Watkins, Maren, Vasconcelos, Vitor, Olivera, Baldomero M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723684/
https://www.ncbi.nlm.nih.gov/pubmed/31344776
http://dx.doi.org/10.3390/md17080432
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author Neves, Jorge L. B.
Imperial, Julita S.
Morgenstern, David
Ueberheide, Beatrix
Gajewiak, Joanna
Antunes, Agostinho
Robinson, Samuel D.
Espino, Samuel
Watkins, Maren
Vasconcelos, Vitor
Olivera, Baldomero M.
author_facet Neves, Jorge L. B.
Imperial, Julita S.
Morgenstern, David
Ueberheide, Beatrix
Gajewiak, Joanna
Antunes, Agostinho
Robinson, Samuel D.
Espino, Samuel
Watkins, Maren
Vasconcelos, Vitor
Olivera, Baldomero M.
author_sort Neves, Jorge L. B.
collection PubMed
description Conus ateralbus is a cone snail endemic to the west side of the island of Sal, in the Cabo Verde Archipelago off West Africa. We describe the isolation and characterization of the first bioactive peptide from the venom of this species. This 30AA venom peptide is named conotoxin AtVIA (δ-conotoxin-like). An excitatory activity was manifested by the peptide on a majority of mouse lumbar dorsal root ganglion neurons. An analog of AtVIA with conservative changes on three amino acid residues at the C-terminal region was synthesized and this analog produced an identical effect on the mouse neurons. AtVIA has homology with δ-conotoxins from other worm-hunters, which include conserved sequence elements that are shared with δ-conotoxins from fish-hunting Conus. In contrast, there is no comparable sequence similarity with δ-conotoxins from the venoms of molluscivorous Conus species. A rationale for the potential presence of δ-conotoxins, that are potent in vertebrate systems in two different lineages of worm-hunting cone snails, is discussed.
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spelling pubmed-67236842019-09-10 Characterization of the First Conotoxin from Conus ateralbus, a Vermivorous Cone Snail from the Cabo Verde Archipelago Neves, Jorge L. B. Imperial, Julita S. Morgenstern, David Ueberheide, Beatrix Gajewiak, Joanna Antunes, Agostinho Robinson, Samuel D. Espino, Samuel Watkins, Maren Vasconcelos, Vitor Olivera, Baldomero M. Mar Drugs Article Conus ateralbus is a cone snail endemic to the west side of the island of Sal, in the Cabo Verde Archipelago off West Africa. We describe the isolation and characterization of the first bioactive peptide from the venom of this species. This 30AA venom peptide is named conotoxin AtVIA (δ-conotoxin-like). An excitatory activity was manifested by the peptide on a majority of mouse lumbar dorsal root ganglion neurons. An analog of AtVIA with conservative changes on three amino acid residues at the C-terminal region was synthesized and this analog produced an identical effect on the mouse neurons. AtVIA has homology with δ-conotoxins from other worm-hunters, which include conserved sequence elements that are shared with δ-conotoxins from fish-hunting Conus. In contrast, there is no comparable sequence similarity with δ-conotoxins from the venoms of molluscivorous Conus species. A rationale for the potential presence of δ-conotoxins, that are potent in vertebrate systems in two different lineages of worm-hunting cone snails, is discussed. MDPI 2019-07-24 /pmc/articles/PMC6723684/ /pubmed/31344776 http://dx.doi.org/10.3390/md17080432 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Neves, Jorge L. B.
Imperial, Julita S.
Morgenstern, David
Ueberheide, Beatrix
Gajewiak, Joanna
Antunes, Agostinho
Robinson, Samuel D.
Espino, Samuel
Watkins, Maren
Vasconcelos, Vitor
Olivera, Baldomero M.
Characterization of the First Conotoxin from Conus ateralbus, a Vermivorous Cone Snail from the Cabo Verde Archipelago
title Characterization of the First Conotoxin from Conus ateralbus, a Vermivorous Cone Snail from the Cabo Verde Archipelago
title_full Characterization of the First Conotoxin from Conus ateralbus, a Vermivorous Cone Snail from the Cabo Verde Archipelago
title_fullStr Characterization of the First Conotoxin from Conus ateralbus, a Vermivorous Cone Snail from the Cabo Verde Archipelago
title_full_unstemmed Characterization of the First Conotoxin from Conus ateralbus, a Vermivorous Cone Snail from the Cabo Verde Archipelago
title_short Characterization of the First Conotoxin from Conus ateralbus, a Vermivorous Cone Snail from the Cabo Verde Archipelago
title_sort characterization of the first conotoxin from conus ateralbus, a vermivorous cone snail from the cabo verde archipelago
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723684/
https://www.ncbi.nlm.nih.gov/pubmed/31344776
http://dx.doi.org/10.3390/md17080432
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