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Similar Allergenicity to Different Artemisia Species Is a Consequence of Highly Cross-Reactive Art v 1-Like Molecules

Background and objectives: Pollens of weeds are relevant elicitors of type I allergies. While many Artemisia species occur worldwide, allergy research so far has only focused on Artemisia vulgaris. We aimed to characterize other prevalent Artemisia species regarding their allergen profiles. Material...

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Autores principales: Pablos, Isabel, Egger, Matthias, Vejvar, Eva, Reichl, Victoria, Briza, Peter, Zennaro, Danila, Rafaiani, Chiara, Pickl, Winfried, Bohle, Barbara, Mari, Adriano, Ferreira, Fatima, Gadermaier, Gabriele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723817/
https://www.ncbi.nlm.nih.gov/pubmed/31434264
http://dx.doi.org/10.3390/medicina55080504
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author Pablos, Isabel
Egger, Matthias
Vejvar, Eva
Reichl, Victoria
Briza, Peter
Zennaro, Danila
Rafaiani, Chiara
Pickl, Winfried
Bohle, Barbara
Mari, Adriano
Ferreira, Fatima
Gadermaier, Gabriele
author_facet Pablos, Isabel
Egger, Matthias
Vejvar, Eva
Reichl, Victoria
Briza, Peter
Zennaro, Danila
Rafaiani, Chiara
Pickl, Winfried
Bohle, Barbara
Mari, Adriano
Ferreira, Fatima
Gadermaier, Gabriele
author_sort Pablos, Isabel
collection PubMed
description Background and objectives: Pollens of weeds are relevant elicitors of type I allergies. While many Artemisia species occur worldwide, allergy research so far has only focused on Artemisia vulgaris. We aimed to characterize other prevalent Artemisia species regarding their allergen profiles. Materials and Methods: Aqueous extracts of pollen from seven Artemisia species were characterized by gel electrophoresis and ELISA using sera from mugwort pollen-allergic patients (n = 11). The cDNA sequences of defensin–proline-linked proteins (DPLPs) were obtained, and purified proteins were tested in a competition ELISA, in rat basophil mediator release assays, and for activation of Jurkat T cells transduced with an Art v 1-specific TCR. IgE cross-reactivity to other allergens was evaluated using ImmunoCAP and ISAC. Results: The protein patterns of Artemisia spp. pollen extracts were similar in gel electrophoresis, with a major band at 24 kDa corresponding to DPLPs, like the previously identified Art v 1. Natural Art v 1 potently inhibited IgE binding to immobilized pollen extracts. Six novel Art v 1 homologs with high sequence identity and equivalent IgE reactivity were identified and termed Art ab 1, Art an 1, Art c 1, Art f 1, Art l 1, and Art t 1. All proteins triggered mediator release and cross-reacted at the T cell level. The Artemisia extracts contained additional IgE cross-reactive molecules from the nonspecific lipid transfer protein, pectate lyase, profilin, and polcalcin family. Conclusions: Our findings demonstrate that DPLPs in various Artemisia species have high allergenic potential. Therefore, related Artemisia species need to be considered to be allergen elicitors, especially due to the consideration of potential geographic expansion due to climatic changes.
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spelling pubmed-67238172019-09-10 Similar Allergenicity to Different Artemisia Species Is a Consequence of Highly Cross-Reactive Art v 1-Like Molecules Pablos, Isabel Egger, Matthias Vejvar, Eva Reichl, Victoria Briza, Peter Zennaro, Danila Rafaiani, Chiara Pickl, Winfried Bohle, Barbara Mari, Adriano Ferreira, Fatima Gadermaier, Gabriele Medicina (Kaunas) Article Background and objectives: Pollens of weeds are relevant elicitors of type I allergies. While many Artemisia species occur worldwide, allergy research so far has only focused on Artemisia vulgaris. We aimed to characterize other prevalent Artemisia species regarding their allergen profiles. Materials and Methods: Aqueous extracts of pollen from seven Artemisia species were characterized by gel electrophoresis and ELISA using sera from mugwort pollen-allergic patients (n = 11). The cDNA sequences of defensin–proline-linked proteins (DPLPs) were obtained, and purified proteins were tested in a competition ELISA, in rat basophil mediator release assays, and for activation of Jurkat T cells transduced with an Art v 1-specific TCR. IgE cross-reactivity to other allergens was evaluated using ImmunoCAP and ISAC. Results: The protein patterns of Artemisia spp. pollen extracts were similar in gel electrophoresis, with a major band at 24 kDa corresponding to DPLPs, like the previously identified Art v 1. Natural Art v 1 potently inhibited IgE binding to immobilized pollen extracts. Six novel Art v 1 homologs with high sequence identity and equivalent IgE reactivity were identified and termed Art ab 1, Art an 1, Art c 1, Art f 1, Art l 1, and Art t 1. All proteins triggered mediator release and cross-reacted at the T cell level. The Artemisia extracts contained additional IgE cross-reactive molecules from the nonspecific lipid transfer protein, pectate lyase, profilin, and polcalcin family. Conclusions: Our findings demonstrate that DPLPs in various Artemisia species have high allergenic potential. Therefore, related Artemisia species need to be considered to be allergen elicitors, especially due to the consideration of potential geographic expansion due to climatic changes. MDPI 2019-08-20 /pmc/articles/PMC6723817/ /pubmed/31434264 http://dx.doi.org/10.3390/medicina55080504 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pablos, Isabel
Egger, Matthias
Vejvar, Eva
Reichl, Victoria
Briza, Peter
Zennaro, Danila
Rafaiani, Chiara
Pickl, Winfried
Bohle, Barbara
Mari, Adriano
Ferreira, Fatima
Gadermaier, Gabriele
Similar Allergenicity to Different Artemisia Species Is a Consequence of Highly Cross-Reactive Art v 1-Like Molecules
title Similar Allergenicity to Different Artemisia Species Is a Consequence of Highly Cross-Reactive Art v 1-Like Molecules
title_full Similar Allergenicity to Different Artemisia Species Is a Consequence of Highly Cross-Reactive Art v 1-Like Molecules
title_fullStr Similar Allergenicity to Different Artemisia Species Is a Consequence of Highly Cross-Reactive Art v 1-Like Molecules
title_full_unstemmed Similar Allergenicity to Different Artemisia Species Is a Consequence of Highly Cross-Reactive Art v 1-Like Molecules
title_short Similar Allergenicity to Different Artemisia Species Is a Consequence of Highly Cross-Reactive Art v 1-Like Molecules
title_sort similar allergenicity to different artemisia species is a consequence of highly cross-reactive art v 1-like molecules
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723817/
https://www.ncbi.nlm.nih.gov/pubmed/31434264
http://dx.doi.org/10.3390/medicina55080504
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