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Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion
Papillomaviruses (PVs) have been identified in a wide range of animal species and are associated with a variety of disease syndromes including classical papillomatosis, aural plaques, and genital papillomas. In horses, 13 PVs have been described to date, falling into six genera. Using total RNA sequ...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723834/ https://www.ncbi.nlm.nih.gov/pubmed/31382657 http://dx.doi.org/10.3390/v11080713 |
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author | Li, Ci-Xiu Chang, Wei-Shan Mitsakos, Katerina Rodger, James Holmes, Edward C. Hudson, Bernard J. |
author_facet | Li, Ci-Xiu Chang, Wei-Shan Mitsakos, Katerina Rodger, James Holmes, Edward C. Hudson, Bernard J. |
author_sort | Li, Ci-Xiu |
collection | PubMed |
description | Papillomaviruses (PVs) have been identified in a wide range of animal species and are associated with a variety of disease syndromes including classical papillomatosis, aural plaques, and genital papillomas. In horses, 13 PVs have been described to date, falling into six genera. Using total RNA sequencing (meta-transcriptomics) we identified a novel equine papillomavirus in semen taken from a thoroughbred stallion suffering a genital lesion, which was confirmed by nested RT-PCR. We designate this novel virus Equus caballus papillomavirus 9 (EcPV9). The complete 7656 bp genome of EcPV9 exhibited similar characteristics to those of other horse papillomaviruses. Phylogenetic analysis based on concatenated E1-E2-L2-L1 amino acid sequences revealed that EcPV9 clustered with EcPV2, EcPV4, and EcPV5, although was distinct enough to represent a new viral species within the genus Dyoiotapapillomavirus (69.35%, 59.25%, and 58.00% nucleotide similarity to EcPV2, EcPV4, and EcPV5, respectively). In sum, we demonstrate the presence of a novel equine papillomavirus for which more detailed studies of disease association are merited. |
format | Online Article Text |
id | pubmed-6723834 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67238342019-09-10 Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion Li, Ci-Xiu Chang, Wei-Shan Mitsakos, Katerina Rodger, James Holmes, Edward C. Hudson, Bernard J. Viruses Communication Papillomaviruses (PVs) have been identified in a wide range of animal species and are associated with a variety of disease syndromes including classical papillomatosis, aural plaques, and genital papillomas. In horses, 13 PVs have been described to date, falling into six genera. Using total RNA sequencing (meta-transcriptomics) we identified a novel equine papillomavirus in semen taken from a thoroughbred stallion suffering a genital lesion, which was confirmed by nested RT-PCR. We designate this novel virus Equus caballus papillomavirus 9 (EcPV9). The complete 7656 bp genome of EcPV9 exhibited similar characteristics to those of other horse papillomaviruses. Phylogenetic analysis based on concatenated E1-E2-L2-L1 amino acid sequences revealed that EcPV9 clustered with EcPV2, EcPV4, and EcPV5, although was distinct enough to represent a new viral species within the genus Dyoiotapapillomavirus (69.35%, 59.25%, and 58.00% nucleotide similarity to EcPV2, EcPV4, and EcPV5, respectively). In sum, we demonstrate the presence of a novel equine papillomavirus for which more detailed studies of disease association are merited. MDPI 2019-08-04 /pmc/articles/PMC6723834/ /pubmed/31382657 http://dx.doi.org/10.3390/v11080713 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Li, Ci-Xiu Chang, Wei-Shan Mitsakos, Katerina Rodger, James Holmes, Edward C. Hudson, Bernard J. Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion |
title | Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion |
title_full | Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion |
title_fullStr | Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion |
title_full_unstemmed | Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion |
title_short | Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion |
title_sort | identification of a novel equine papillomavirus in semen from a thoroughbred stallion with a penile lesion |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723834/ https://www.ncbi.nlm.nih.gov/pubmed/31382657 http://dx.doi.org/10.3390/v11080713 |
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