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Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion

Papillomaviruses (PVs) have been identified in a wide range of animal species and are associated with a variety of disease syndromes including classical papillomatosis, aural plaques, and genital papillomas. In horses, 13 PVs have been described to date, falling into six genera. Using total RNA sequ...

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Autores principales: Li, Ci-Xiu, Chang, Wei-Shan, Mitsakos, Katerina, Rodger, James, Holmes, Edward C., Hudson, Bernard J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723834/
https://www.ncbi.nlm.nih.gov/pubmed/31382657
http://dx.doi.org/10.3390/v11080713
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author Li, Ci-Xiu
Chang, Wei-Shan
Mitsakos, Katerina
Rodger, James
Holmes, Edward C.
Hudson, Bernard J.
author_facet Li, Ci-Xiu
Chang, Wei-Shan
Mitsakos, Katerina
Rodger, James
Holmes, Edward C.
Hudson, Bernard J.
author_sort Li, Ci-Xiu
collection PubMed
description Papillomaviruses (PVs) have been identified in a wide range of animal species and are associated with a variety of disease syndromes including classical papillomatosis, aural plaques, and genital papillomas. In horses, 13 PVs have been described to date, falling into six genera. Using total RNA sequencing (meta-transcriptomics) we identified a novel equine papillomavirus in semen taken from a thoroughbred stallion suffering a genital lesion, which was confirmed by nested RT-PCR. We designate this novel virus Equus caballus papillomavirus 9 (EcPV9). The complete 7656 bp genome of EcPV9 exhibited similar characteristics to those of other horse papillomaviruses. Phylogenetic analysis based on concatenated E1-E2-L2-L1 amino acid sequences revealed that EcPV9 clustered with EcPV2, EcPV4, and EcPV5, although was distinct enough to represent a new viral species within the genus Dyoiotapapillomavirus (69.35%, 59.25%, and 58.00% nucleotide similarity to EcPV2, EcPV4, and EcPV5, respectively). In sum, we demonstrate the presence of a novel equine papillomavirus for which more detailed studies of disease association are merited.
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spelling pubmed-67238342019-09-10 Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion Li, Ci-Xiu Chang, Wei-Shan Mitsakos, Katerina Rodger, James Holmes, Edward C. Hudson, Bernard J. Viruses Communication Papillomaviruses (PVs) have been identified in a wide range of animal species and are associated with a variety of disease syndromes including classical papillomatosis, aural plaques, and genital papillomas. In horses, 13 PVs have been described to date, falling into six genera. Using total RNA sequencing (meta-transcriptomics) we identified a novel equine papillomavirus in semen taken from a thoroughbred stallion suffering a genital lesion, which was confirmed by nested RT-PCR. We designate this novel virus Equus caballus papillomavirus 9 (EcPV9). The complete 7656 bp genome of EcPV9 exhibited similar characteristics to those of other horse papillomaviruses. Phylogenetic analysis based on concatenated E1-E2-L2-L1 amino acid sequences revealed that EcPV9 clustered with EcPV2, EcPV4, and EcPV5, although was distinct enough to represent a new viral species within the genus Dyoiotapapillomavirus (69.35%, 59.25%, and 58.00% nucleotide similarity to EcPV2, EcPV4, and EcPV5, respectively). In sum, we demonstrate the presence of a novel equine papillomavirus for which more detailed studies of disease association are merited. MDPI 2019-08-04 /pmc/articles/PMC6723834/ /pubmed/31382657 http://dx.doi.org/10.3390/v11080713 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Li, Ci-Xiu
Chang, Wei-Shan
Mitsakos, Katerina
Rodger, James
Holmes, Edward C.
Hudson, Bernard J.
Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion
title Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion
title_full Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion
title_fullStr Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion
title_full_unstemmed Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion
title_short Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion
title_sort identification of a novel equine papillomavirus in semen from a thoroughbred stallion with a penile lesion
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723834/
https://www.ncbi.nlm.nih.gov/pubmed/31382657
http://dx.doi.org/10.3390/v11080713
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