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Isolation and Full-Length Sequence Analysis of a Pestivirus from Aborted Lamb Fetuses in Italy

Pestiviruses are distributed worldwide and are responsible for a variety of economically important diseases. They are not very host-specific, and thus sheep can be infected by well-known pestiviruses like bovine viral diarrhea virus (BVDV) and border disease virus (BDV), as well as by other recently...

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Autores principales: Sozzi, Enrica, Lavazza, Antonio, Gaffuri, Alessandra, Bencetti, Fabio Carlo, Prosperi, Alice, Lelli, Davide, Chiapponi, Chiara, Moreno, Ana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723841/
https://www.ncbi.nlm.nih.gov/pubmed/31412585
http://dx.doi.org/10.3390/v11080744
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author Sozzi, Enrica
Lavazza, Antonio
Gaffuri, Alessandra
Bencetti, Fabio Carlo
Prosperi, Alice
Lelli, Davide
Chiapponi, Chiara
Moreno, Ana
author_facet Sozzi, Enrica
Lavazza, Antonio
Gaffuri, Alessandra
Bencetti, Fabio Carlo
Prosperi, Alice
Lelli, Davide
Chiapponi, Chiara
Moreno, Ana
author_sort Sozzi, Enrica
collection PubMed
description Pestiviruses are distributed worldwide and are responsible for a variety of economically important diseases. They are not very host-specific, and thus sheep can be infected by well-known pestiviruses like bovine viral diarrhea virus (BVDV) and border disease virus (BDV), as well as by other recently discovered pestivirus species. The aim of this study is to describe the isolation and characterization of four pestivirus strains detected in aborted lamb fetuses from a single farm in the Brescia province (Northern Italy). A total of twelve aborted fetuses were collected and examined. After necropsy, organs were tested for the presence of infectious agents known as potential causes of abortion (Brucella spp., Listeria spp., Coxiella burnetii, Chlamydophila spp., Mycoplasma spp., Neospora caninum, and Toxoplasma gondii), and submitted to viral identification by isolation on Madin Darby bovine kidney (MDBK) cell culture and by PCR assay for Schmallenberg virus and pan-pestivirus RT-PCR real time assay. Three viral strains (Ovine/IT/1756/2017, Ovine/IT/338710-2/2017, and Ovine/IT/338710-3/2017) were isolated in the absence of cytopathic effects (CPEs) in cell cultures and identified with RT-PCR. Another pestivirus strain (Ovine/IT/16235-2/2018) was detected by PCR, but was not successfully isolated. Complete sequence genomic data of the three isolated viruses showed that they were highly similar, differed genetically from known pestivirus species, and were closely related to classical swine fever virus (CSFV). Beyond the identification of new ovine pestiviruses, this study indicates that a systematic diagnostic approach is important to identify the presence and map the distribution of both known and emerging pestiviruses.
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spelling pubmed-67238412019-09-10 Isolation and Full-Length Sequence Analysis of a Pestivirus from Aborted Lamb Fetuses in Italy Sozzi, Enrica Lavazza, Antonio Gaffuri, Alessandra Bencetti, Fabio Carlo Prosperi, Alice Lelli, Davide Chiapponi, Chiara Moreno, Ana Viruses Article Pestiviruses are distributed worldwide and are responsible for a variety of economically important diseases. They are not very host-specific, and thus sheep can be infected by well-known pestiviruses like bovine viral diarrhea virus (BVDV) and border disease virus (BDV), as well as by other recently discovered pestivirus species. The aim of this study is to describe the isolation and characterization of four pestivirus strains detected in aborted lamb fetuses from a single farm in the Brescia province (Northern Italy). A total of twelve aborted fetuses were collected and examined. After necropsy, organs were tested for the presence of infectious agents known as potential causes of abortion (Brucella spp., Listeria spp., Coxiella burnetii, Chlamydophila spp., Mycoplasma spp., Neospora caninum, and Toxoplasma gondii), and submitted to viral identification by isolation on Madin Darby bovine kidney (MDBK) cell culture and by PCR assay for Schmallenberg virus and pan-pestivirus RT-PCR real time assay. Three viral strains (Ovine/IT/1756/2017, Ovine/IT/338710-2/2017, and Ovine/IT/338710-3/2017) were isolated in the absence of cytopathic effects (CPEs) in cell cultures and identified with RT-PCR. Another pestivirus strain (Ovine/IT/16235-2/2018) was detected by PCR, but was not successfully isolated. Complete sequence genomic data of the three isolated viruses showed that they were highly similar, differed genetically from known pestivirus species, and were closely related to classical swine fever virus (CSFV). Beyond the identification of new ovine pestiviruses, this study indicates that a systematic diagnostic approach is important to identify the presence and map the distribution of both known and emerging pestiviruses. MDPI 2019-08-13 /pmc/articles/PMC6723841/ /pubmed/31412585 http://dx.doi.org/10.3390/v11080744 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sozzi, Enrica
Lavazza, Antonio
Gaffuri, Alessandra
Bencetti, Fabio Carlo
Prosperi, Alice
Lelli, Davide
Chiapponi, Chiara
Moreno, Ana
Isolation and Full-Length Sequence Analysis of a Pestivirus from Aborted Lamb Fetuses in Italy
title Isolation and Full-Length Sequence Analysis of a Pestivirus from Aborted Lamb Fetuses in Italy
title_full Isolation and Full-Length Sequence Analysis of a Pestivirus from Aborted Lamb Fetuses in Italy
title_fullStr Isolation and Full-Length Sequence Analysis of a Pestivirus from Aborted Lamb Fetuses in Italy
title_full_unstemmed Isolation and Full-Length Sequence Analysis of a Pestivirus from Aborted Lamb Fetuses in Italy
title_short Isolation and Full-Length Sequence Analysis of a Pestivirus from Aborted Lamb Fetuses in Italy
title_sort isolation and full-length sequence analysis of a pestivirus from aborted lamb fetuses in italy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723841/
https://www.ncbi.nlm.nih.gov/pubmed/31412585
http://dx.doi.org/10.3390/v11080744
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