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The Capability of O-Acetyl-ADP-Ribose, an Epigenetic Metabolic Small Molecule, on Promoting the Further Spreading of Sir3 along the Telomeric Chromatin
O-acetyl-ADP-ribose (AAR) is a metabolic small molecule relevant in epigenetics that is generated by NAD-dependent histone deacetylases, such as Sir2. The formation of silent heterochromatin in yeast requires histone deacetylation by Sir2, structural rearrangement of SIR complexes, spreading of SIR...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723988/ https://www.ncbi.nlm.nih.gov/pubmed/31366171 http://dx.doi.org/10.3390/genes10080577 |
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author | Tung, Shu-Yun Wang, Sue-Hong Lee, Sue-Ping Tsai, Shu-Ping Su, Kuan-Chung Shen, Hsiao-Hsuian Hong, Jia-Yang Tsai, Ming-Shiun Liou, Gunn-Guang |
author_facet | Tung, Shu-Yun Wang, Sue-Hong Lee, Sue-Ping Tsai, Shu-Ping Su, Kuan-Chung Shen, Hsiao-Hsuian Hong, Jia-Yang Tsai, Ming-Shiun Liou, Gunn-Guang |
author_sort | Tung, Shu-Yun |
collection | PubMed |
description | O-acetyl-ADP-ribose (AAR) is a metabolic small molecule relevant in epigenetics that is generated by NAD-dependent histone deacetylases, such as Sir2. The formation of silent heterochromatin in yeast requires histone deacetylation by Sir2, structural rearrangement of SIR complexes, spreading of SIR complexes along the chromatin, and additional maturation processing. AAR affects the interactions of the SIR-nucleosome in vitro and enhances the chromatin epigenetic silencing effect in vivo. In this study, using isothermal titration calorimetry (ITC) and dot blotting methods, we showed the direct interaction of AAR with Sir3. Furthermore, through chromatin immunoprecipitation (ChIP)-on-chip and chromatin affinity purification (ChAP)-on chip assays, we discovered that AAR is capable of increasing the extended spreading of Sir3 along telomeres, but not Sir2. In addition, the findings of a quantitative real-time polymerase chain reaction (qRT-PCR) and examinations of an in vitro assembly system of SIR-nucleosome heterochromatin filament were consistent with these results. This study provides evidence indicating another important effect of AAR in vivo. AAR may play a specific modulating role in the formation of silent SIR-nucleosome heterochromatin in yeast. |
format | Online Article Text |
id | pubmed-6723988 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-67239882019-09-10 The Capability of O-Acetyl-ADP-Ribose, an Epigenetic Metabolic Small Molecule, on Promoting the Further Spreading of Sir3 along the Telomeric Chromatin Tung, Shu-Yun Wang, Sue-Hong Lee, Sue-Ping Tsai, Shu-Ping Su, Kuan-Chung Shen, Hsiao-Hsuian Hong, Jia-Yang Tsai, Ming-Shiun Liou, Gunn-Guang Genes (Basel) Article O-acetyl-ADP-ribose (AAR) is a metabolic small molecule relevant in epigenetics that is generated by NAD-dependent histone deacetylases, such as Sir2. The formation of silent heterochromatin in yeast requires histone deacetylation by Sir2, structural rearrangement of SIR complexes, spreading of SIR complexes along the chromatin, and additional maturation processing. AAR affects the interactions of the SIR-nucleosome in vitro and enhances the chromatin epigenetic silencing effect in vivo. In this study, using isothermal titration calorimetry (ITC) and dot blotting methods, we showed the direct interaction of AAR with Sir3. Furthermore, through chromatin immunoprecipitation (ChIP)-on-chip and chromatin affinity purification (ChAP)-on chip assays, we discovered that AAR is capable of increasing the extended spreading of Sir3 along telomeres, but not Sir2. In addition, the findings of a quantitative real-time polymerase chain reaction (qRT-PCR) and examinations of an in vitro assembly system of SIR-nucleosome heterochromatin filament were consistent with these results. This study provides evidence indicating another important effect of AAR in vivo. AAR may play a specific modulating role in the formation of silent SIR-nucleosome heterochromatin in yeast. MDPI 2019-07-30 /pmc/articles/PMC6723988/ /pubmed/31366171 http://dx.doi.org/10.3390/genes10080577 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tung, Shu-Yun Wang, Sue-Hong Lee, Sue-Ping Tsai, Shu-Ping Su, Kuan-Chung Shen, Hsiao-Hsuian Hong, Jia-Yang Tsai, Ming-Shiun Liou, Gunn-Guang The Capability of O-Acetyl-ADP-Ribose, an Epigenetic Metabolic Small Molecule, on Promoting the Further Spreading of Sir3 along the Telomeric Chromatin |
title | The Capability of O-Acetyl-ADP-Ribose, an Epigenetic Metabolic Small Molecule, on Promoting the Further Spreading of Sir3 along the Telomeric Chromatin |
title_full | The Capability of O-Acetyl-ADP-Ribose, an Epigenetic Metabolic Small Molecule, on Promoting the Further Spreading of Sir3 along the Telomeric Chromatin |
title_fullStr | The Capability of O-Acetyl-ADP-Ribose, an Epigenetic Metabolic Small Molecule, on Promoting the Further Spreading of Sir3 along the Telomeric Chromatin |
title_full_unstemmed | The Capability of O-Acetyl-ADP-Ribose, an Epigenetic Metabolic Small Molecule, on Promoting the Further Spreading of Sir3 along the Telomeric Chromatin |
title_short | The Capability of O-Acetyl-ADP-Ribose, an Epigenetic Metabolic Small Molecule, on Promoting the Further Spreading of Sir3 along the Telomeric Chromatin |
title_sort | capability of o-acetyl-adp-ribose, an epigenetic metabolic small molecule, on promoting the further spreading of sir3 along the telomeric chromatin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723988/ https://www.ncbi.nlm.nih.gov/pubmed/31366171 http://dx.doi.org/10.3390/genes10080577 |
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