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Oxidative Stress Response of Aspergillus oryzae Induced by Hydrogen Peroxide and Menadione Sodium Bisulfite

Oxidative stress response protects organisms from deleterious effects of reactive oxygen species (ROS), which can damage cellular components and cause disturbance of the cellular homeostasis. Although the defensive biochemical mechanisms have been extensively studied in yeast and other filamentous f...

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Autores principales: Shao, Huanhuan, Tu, Yayi, Wang, Yijing, Jiang, Chunmiao, Ma, Long, Hu, Zhihong, Wang, Jiangfan, Zeng, Bin, He, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724031/
https://www.ncbi.nlm.nih.gov/pubmed/31366149
http://dx.doi.org/10.3390/microorganisms7080225
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author Shao, Huanhuan
Tu, Yayi
Wang, Yijing
Jiang, Chunmiao
Ma, Long
Hu, Zhihong
Wang, Jiangfan
Zeng, Bin
He, Bin
author_facet Shao, Huanhuan
Tu, Yayi
Wang, Yijing
Jiang, Chunmiao
Ma, Long
Hu, Zhihong
Wang, Jiangfan
Zeng, Bin
He, Bin
author_sort Shao, Huanhuan
collection PubMed
description Oxidative stress response protects organisms from deleterious effects of reactive oxygen species (ROS), which can damage cellular components and cause disturbance of the cellular homeostasis. Although the defensive biochemical mechanisms have been extensively studied in yeast and other filamentous fungi, little information is available about Aspergillus oryzae. We investigated the effect of two oxidant agents (menadione sodium bisulfite, MSB, and hydrogen peroxide, H(2)O(2)) on cellular growth and antioxidant enzyme induction in A. oryzae. Results indicated severe inhibition of biomass and conidia production when high concentration of oxidants was used. Transcriptomic analysis showed an up-regulated expression of genes involved in oxidoreduction, such as catalase, glutathione peroxidase, and superoxide dismutase. In addition, it was observed that oxidative stress stimuli enhanced the expression of Yap1 and Skn7 transcription factors. Further, metabolomic analysis showed that glutathione content was increased in the oxidative treatments when compared with the control. Moreover, the content of unsaturated fatty acid decreased with oxidative treatment accompanying with the down-regulated expression of genes involved in linoleic acid biosynthesis. This study provided a global transcriptome characterization of oxidative stress response in A. oryzae, and can offer multiple target genes for oxidative tolerance improvement via genetic engineering.
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spelling pubmed-67240312019-09-10 Oxidative Stress Response of Aspergillus oryzae Induced by Hydrogen Peroxide and Menadione Sodium Bisulfite Shao, Huanhuan Tu, Yayi Wang, Yijing Jiang, Chunmiao Ma, Long Hu, Zhihong Wang, Jiangfan Zeng, Bin He, Bin Microorganisms Article Oxidative stress response protects organisms from deleterious effects of reactive oxygen species (ROS), which can damage cellular components and cause disturbance of the cellular homeostasis. Although the defensive biochemical mechanisms have been extensively studied in yeast and other filamentous fungi, little information is available about Aspergillus oryzae. We investigated the effect of two oxidant agents (menadione sodium bisulfite, MSB, and hydrogen peroxide, H(2)O(2)) on cellular growth and antioxidant enzyme induction in A. oryzae. Results indicated severe inhibition of biomass and conidia production when high concentration of oxidants was used. Transcriptomic analysis showed an up-regulated expression of genes involved in oxidoreduction, such as catalase, glutathione peroxidase, and superoxide dismutase. In addition, it was observed that oxidative stress stimuli enhanced the expression of Yap1 and Skn7 transcription factors. Further, metabolomic analysis showed that glutathione content was increased in the oxidative treatments when compared with the control. Moreover, the content of unsaturated fatty acid decreased with oxidative treatment accompanying with the down-regulated expression of genes involved in linoleic acid biosynthesis. This study provided a global transcriptome characterization of oxidative stress response in A. oryzae, and can offer multiple target genes for oxidative tolerance improvement via genetic engineering. MDPI 2019-07-30 /pmc/articles/PMC6724031/ /pubmed/31366149 http://dx.doi.org/10.3390/microorganisms7080225 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shao, Huanhuan
Tu, Yayi
Wang, Yijing
Jiang, Chunmiao
Ma, Long
Hu, Zhihong
Wang, Jiangfan
Zeng, Bin
He, Bin
Oxidative Stress Response of Aspergillus oryzae Induced by Hydrogen Peroxide and Menadione Sodium Bisulfite
title Oxidative Stress Response of Aspergillus oryzae Induced by Hydrogen Peroxide and Menadione Sodium Bisulfite
title_full Oxidative Stress Response of Aspergillus oryzae Induced by Hydrogen Peroxide and Menadione Sodium Bisulfite
title_fullStr Oxidative Stress Response of Aspergillus oryzae Induced by Hydrogen Peroxide and Menadione Sodium Bisulfite
title_full_unstemmed Oxidative Stress Response of Aspergillus oryzae Induced by Hydrogen Peroxide and Menadione Sodium Bisulfite
title_short Oxidative Stress Response of Aspergillus oryzae Induced by Hydrogen Peroxide and Menadione Sodium Bisulfite
title_sort oxidative stress response of aspergillus oryzae induced by hydrogen peroxide and menadione sodium bisulfite
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724031/
https://www.ncbi.nlm.nih.gov/pubmed/31366149
http://dx.doi.org/10.3390/microorganisms7080225
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