Cargando…

Highly Effective and Noninvasive Near‐Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min

Biofilms have been related to the persistence of infections on medical implants, and these cannot be eradicated because of the resistance of biofilm structures. Therefore, a biocompatible phototherapeutic system is developed composed of MoS(2), IR780 photosensitizer, and arginine–glycine–aspartic ac...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Mu, Li, Liqian, Su, Kun, Liu, Xiangmei, Zhang, Tianjin, Liang, Yanqin, Jing, Doudou, Yang, Xianjin, Zheng, Dong, Cui, Zhenduo, Li, Zhaoyang, Zhu, Shengli, Yeung, Kelvin Wai Kwok, Zheng, Yufeng, Wang, Xianbao, Wu, Shuilin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724470/
https://www.ncbi.nlm.nih.gov/pubmed/31508278
http://dx.doi.org/10.1002/advs.201900599
_version_ 1783449000857305088
author Li, Mu
Li, Liqian
Su, Kun
Liu, Xiangmei
Zhang, Tianjin
Liang, Yanqin
Jing, Doudou
Yang, Xianjin
Zheng, Dong
Cui, Zhenduo
Li, Zhaoyang
Zhu, Shengli
Yeung, Kelvin Wai Kwok
Zheng, Yufeng
Wang, Xianbao
Wu, Shuilin
author_facet Li, Mu
Li, Liqian
Su, Kun
Liu, Xiangmei
Zhang, Tianjin
Liang, Yanqin
Jing, Doudou
Yang, Xianjin
Zheng, Dong
Cui, Zhenduo
Li, Zhaoyang
Zhu, Shengli
Yeung, Kelvin Wai Kwok
Zheng, Yufeng
Wang, Xianbao
Wu, Shuilin
author_sort Li, Mu
collection PubMed
description Biofilms have been related to the persistence of infections on medical implants, and these cannot be eradicated because of the resistance of biofilm structures. Therefore, a biocompatible phototherapeutic system is developed composed of MoS(2), IR780 photosensitizer, and arginine–glycine–aspartic acid–cysteine (RGDC) to safely eradicate biofilms on titanium implants within 20 min. The magnetron‐sputtered MoS(2) film possesses excellent photothermal properties, and IR780 can produce reactive oxygen species (ROS) with the irradiation of near‐infrared (NIR, λ = 700–1100 nm) light. Consequently, the combination of photothermal therapy (PTT) and photodynamic therapy (PDT), assisted by glutathione oxidation accelerated by NIR light, can provide synergistic and rapid killing of bacteria, i.e., 98.99 ± 0.42% eradication ratio against a Staphylococcus aureus biofilm in vivo within 20 min, which is much greater than that of PTT or PDT alone. With the assistance of ROS, the permeability of damaged bacterial membranes increases, and the damaged bacterial membranes become more sensitive to heat, thus accelerating the leakage of proteins from the bacteria. In addition, RGDC can provide excellent biosafety and osteoconductivity, which is confirmed by in vivo animal experiments.
format Online
Article
Text
id pubmed-6724470
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-67244702019-09-10 Highly Effective and Noninvasive Near‐Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min Li, Mu Li, Liqian Su, Kun Liu, Xiangmei Zhang, Tianjin Liang, Yanqin Jing, Doudou Yang, Xianjin Zheng, Dong Cui, Zhenduo Li, Zhaoyang Zhu, Shengli Yeung, Kelvin Wai Kwok Zheng, Yufeng Wang, Xianbao Wu, Shuilin Adv Sci (Weinh) Full Papers Biofilms have been related to the persistence of infections on medical implants, and these cannot be eradicated because of the resistance of biofilm structures. Therefore, a biocompatible phototherapeutic system is developed composed of MoS(2), IR780 photosensitizer, and arginine–glycine–aspartic acid–cysteine (RGDC) to safely eradicate biofilms on titanium implants within 20 min. The magnetron‐sputtered MoS(2) film possesses excellent photothermal properties, and IR780 can produce reactive oxygen species (ROS) with the irradiation of near‐infrared (NIR, λ = 700–1100 nm) light. Consequently, the combination of photothermal therapy (PTT) and photodynamic therapy (PDT), assisted by glutathione oxidation accelerated by NIR light, can provide synergistic and rapid killing of bacteria, i.e., 98.99 ± 0.42% eradication ratio against a Staphylococcus aureus biofilm in vivo within 20 min, which is much greater than that of PTT or PDT alone. With the assistance of ROS, the permeability of damaged bacterial membranes increases, and the damaged bacterial membranes become more sensitive to heat, thus accelerating the leakage of proteins from the bacteria. In addition, RGDC can provide excellent biosafety and osteoconductivity, which is confirmed by in vivo animal experiments. John Wiley and Sons Inc. 2019-07-19 /pmc/articles/PMC6724470/ /pubmed/31508278 http://dx.doi.org/10.1002/advs.201900599 Text en © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Papers
Li, Mu
Li, Liqian
Su, Kun
Liu, Xiangmei
Zhang, Tianjin
Liang, Yanqin
Jing, Doudou
Yang, Xianjin
Zheng, Dong
Cui, Zhenduo
Li, Zhaoyang
Zhu, Shengli
Yeung, Kelvin Wai Kwok
Zheng, Yufeng
Wang, Xianbao
Wu, Shuilin
Highly Effective and Noninvasive Near‐Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min
title Highly Effective and Noninvasive Near‐Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min
title_full Highly Effective and Noninvasive Near‐Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min
title_fullStr Highly Effective and Noninvasive Near‐Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min
title_full_unstemmed Highly Effective and Noninvasive Near‐Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min
title_short Highly Effective and Noninvasive Near‐Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min
title_sort highly effective and noninvasive near‐infrared eradication of a staphylococcus aureus biofilm on implants by a photoresponsive coating within 20 min
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724470/
https://www.ncbi.nlm.nih.gov/pubmed/31508278
http://dx.doi.org/10.1002/advs.201900599
work_keys_str_mv AT limu highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min
AT liliqian highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min
AT sukun highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min
AT liuxiangmei highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min
AT zhangtianjin highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min
AT liangyanqin highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min
AT jingdoudou highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min
AT yangxianjin highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min
AT zhengdong highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min
AT cuizhenduo highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min
AT lizhaoyang highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min
AT zhushengli highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min
AT yeungkelvinwaikwok highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min
AT zhengyufeng highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min
AT wangxianbao highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min
AT wushuilin highlyeffectiveandnoninvasivenearinfrarederadicationofastaphylococcusaureusbiofilmonimplantsbyaphotoresponsivecoatingwithin20min