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Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms

Two-dimensional (2D) substrate rigidity promotes myosin II activity to increase traction force in a process negatively regulated by tropomyosin (Tpm) 2.1. We recently discovered that actomyosin contractility can increase intracellular pressure and switch tumor cells from low-pressure lamellipodia to...

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Autores principales: Sao, Kimheak, Jones, Tia M., Doyle, Andrew D., Maity, Debonil, Schevzov, Galina, Chen, Yun, Gunning, Peter W., Petrie, Ryan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724525/
https://www.ncbi.nlm.nih.gov/pubmed/30865560
http://dx.doi.org/10.1091/mbc.E18-06-0355
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author Sao, Kimheak
Jones, Tia M.
Doyle, Andrew D.
Maity, Debonil
Schevzov, Galina
Chen, Yun
Gunning, Peter W.
Petrie, Ryan J.
author_facet Sao, Kimheak
Jones, Tia M.
Doyle, Andrew D.
Maity, Debonil
Schevzov, Galina
Chen, Yun
Gunning, Peter W.
Petrie, Ryan J.
author_sort Sao, Kimheak
collection PubMed
description Two-dimensional (2D) substrate rigidity promotes myosin II activity to increase traction force in a process negatively regulated by tropomyosin (Tpm) 2.1. We recently discovered that actomyosin contractility can increase intracellular pressure and switch tumor cells from low-pressure lamellipodia to high-pressure lobopodial protrusions during three-dimensional (3D) migration. However, it remains unclear whether these myosin II–generated cellular forces are produced simultaneously, and by the same molecular machinery. Here we identify Tpm 1.6 as a positive regulator of intracellular pressure and confirm that Tpm 2.1 is a negative regulator of traction force. We find that Tpm 1.6 and 2.1 can control intracellular pressure and traction independently, suggesting these myosin II–dependent forces are generated by distinct mechanisms. Further, these tropomyosin-regulated mechanisms can be integrated to control complex cell behaviors on 2D and in 3D environments.
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spelling pubmed-67245252019-09-05 Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms Sao, Kimheak Jones, Tia M. Doyle, Andrew D. Maity, Debonil Schevzov, Galina Chen, Yun Gunning, Peter W. Petrie, Ryan J. Mol Biol Cell Articles Two-dimensional (2D) substrate rigidity promotes myosin II activity to increase traction force in a process negatively regulated by tropomyosin (Tpm) 2.1. We recently discovered that actomyosin contractility can increase intracellular pressure and switch tumor cells from low-pressure lamellipodia to high-pressure lobopodial protrusions during three-dimensional (3D) migration. However, it remains unclear whether these myosin II–generated cellular forces are produced simultaneously, and by the same molecular machinery. Here we identify Tpm 1.6 as a positive regulator of intracellular pressure and confirm that Tpm 2.1 is a negative regulator of traction force. We find that Tpm 1.6 and 2.1 can control intracellular pressure and traction independently, suggesting these myosin II–dependent forces are generated by distinct mechanisms. Further, these tropomyosin-regulated mechanisms can be integrated to control complex cell behaviors on 2D and in 3D environments. The American Society for Cell Biology 2019-05-01 /pmc/articles/PMC6724525/ /pubmed/30865560 http://dx.doi.org/10.1091/mbc.E18-06-0355 Text en © 2019 Sao et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Sao, Kimheak
Jones, Tia M.
Doyle, Andrew D.
Maity, Debonil
Schevzov, Galina
Chen, Yun
Gunning, Peter W.
Petrie, Ryan J.
Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms
title Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms
title_full Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms
title_fullStr Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms
title_full_unstemmed Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms
title_short Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms
title_sort myosin ii governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724525/
https://www.ncbi.nlm.nih.gov/pubmed/30865560
http://dx.doi.org/10.1091/mbc.E18-06-0355
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