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Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms
Two-dimensional (2D) substrate rigidity promotes myosin II activity to increase traction force in a process negatively regulated by tropomyosin (Tpm) 2.1. We recently discovered that actomyosin contractility can increase intracellular pressure and switch tumor cells from low-pressure lamellipodia to...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724525/ https://www.ncbi.nlm.nih.gov/pubmed/30865560 http://dx.doi.org/10.1091/mbc.E18-06-0355 |
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author | Sao, Kimheak Jones, Tia M. Doyle, Andrew D. Maity, Debonil Schevzov, Galina Chen, Yun Gunning, Peter W. Petrie, Ryan J. |
author_facet | Sao, Kimheak Jones, Tia M. Doyle, Andrew D. Maity, Debonil Schevzov, Galina Chen, Yun Gunning, Peter W. Petrie, Ryan J. |
author_sort | Sao, Kimheak |
collection | PubMed |
description | Two-dimensional (2D) substrate rigidity promotes myosin II activity to increase traction force in a process negatively regulated by tropomyosin (Tpm) 2.1. We recently discovered that actomyosin contractility can increase intracellular pressure and switch tumor cells from low-pressure lamellipodia to high-pressure lobopodial protrusions during three-dimensional (3D) migration. However, it remains unclear whether these myosin II–generated cellular forces are produced simultaneously, and by the same molecular machinery. Here we identify Tpm 1.6 as a positive regulator of intracellular pressure and confirm that Tpm 2.1 is a negative regulator of traction force. We find that Tpm 1.6 and 2.1 can control intracellular pressure and traction independently, suggesting these myosin II–dependent forces are generated by distinct mechanisms. Further, these tropomyosin-regulated mechanisms can be integrated to control complex cell behaviors on 2D and in 3D environments. |
format | Online Article Text |
id | pubmed-6724525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-67245252019-09-05 Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms Sao, Kimheak Jones, Tia M. Doyle, Andrew D. Maity, Debonil Schevzov, Galina Chen, Yun Gunning, Peter W. Petrie, Ryan J. Mol Biol Cell Articles Two-dimensional (2D) substrate rigidity promotes myosin II activity to increase traction force in a process negatively regulated by tropomyosin (Tpm) 2.1. We recently discovered that actomyosin contractility can increase intracellular pressure and switch tumor cells from low-pressure lamellipodia to high-pressure lobopodial protrusions during three-dimensional (3D) migration. However, it remains unclear whether these myosin II–generated cellular forces are produced simultaneously, and by the same molecular machinery. Here we identify Tpm 1.6 as a positive regulator of intracellular pressure and confirm that Tpm 2.1 is a negative regulator of traction force. We find that Tpm 1.6 and 2.1 can control intracellular pressure and traction independently, suggesting these myosin II–dependent forces are generated by distinct mechanisms. Further, these tropomyosin-regulated mechanisms can be integrated to control complex cell behaviors on 2D and in 3D environments. The American Society for Cell Biology 2019-05-01 /pmc/articles/PMC6724525/ /pubmed/30865560 http://dx.doi.org/10.1091/mbc.E18-06-0355 Text en © 2019 Sao et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License. |
spellingShingle | Articles Sao, Kimheak Jones, Tia M. Doyle, Andrew D. Maity, Debonil Schevzov, Galina Chen, Yun Gunning, Peter W. Petrie, Ryan J. Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms |
title | Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms |
title_full | Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms |
title_fullStr | Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms |
title_full_unstemmed | Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms |
title_short | Myosin II governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms |
title_sort | myosin ii governs intracellular pressure and traction by distinct tropomyosin-dependent mechanisms |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724525/ https://www.ncbi.nlm.nih.gov/pubmed/30865560 http://dx.doi.org/10.1091/mbc.E18-06-0355 |
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