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Microbial Community Profiling in Intensive Care Units Expose Limitations in Current Sanitary Standards

Hospital-associated infections (HAIs) are a leading cause of morbidity and mortality in intensive care units (ICUs) and neonatal intensive care units (NICUs). Organisms causing these infections are often present on surfaces around the patient. Given that microbiota may vary across different ICUs, th...

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Autores principales: Ribeiro, Lucas Ferreira, Lopes, Erica M., Kishi, Luciano T., Ribeiro, Liliane Fraga Costa, Menegueti, Mayra Gonçalves, Gaspar, Gilberto Gambero, Silva-Rocha, Rafael, Guazzaroni, María-Eugenia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724580/
https://www.ncbi.nlm.nih.gov/pubmed/31555629
http://dx.doi.org/10.3389/fpubh.2019.00240
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author Ribeiro, Lucas Ferreira
Lopes, Erica M.
Kishi, Luciano T.
Ribeiro, Liliane Fraga Costa
Menegueti, Mayra Gonçalves
Gaspar, Gilberto Gambero
Silva-Rocha, Rafael
Guazzaroni, María-Eugenia
author_facet Ribeiro, Lucas Ferreira
Lopes, Erica M.
Kishi, Luciano T.
Ribeiro, Liliane Fraga Costa
Menegueti, Mayra Gonçalves
Gaspar, Gilberto Gambero
Silva-Rocha, Rafael
Guazzaroni, María-Eugenia
author_sort Ribeiro, Lucas Ferreira
collection PubMed
description Hospital-associated infections (HAIs) are a leading cause of morbidity and mortality in intensive care units (ICUs) and neonatal intensive care units (NICUs). Organisms causing these infections are often present on surfaces around the patient. Given that microbiota may vary across different ICUs, the HAI-related microbial signatures within these units remain underexplored. In this study, we use deep-sequencing analyses to explore and compare the structure of bacterial communities at inanimate surfaces of the ICU and NICU wards of The Medical School Clinics Hospital (Brazil). The data revealed that NICU presents higher biodiversity than ICU and surfaces closest to the patient showed a peculiar microbiota, distinguishing one unit from the other. Several facultative anaerobes or obligate anaerobes HAI-related genera were classified as biomarkers for the NICU, whereas Pseudomonas was the main biomarker for ICU. Correlation analyses revealed a distinct pattern of microbe-microbe interactions for each unit, including bacteria able to form multi-genera biofilms. Furthermore, we evaluated the effect of concurrent cleaning over the ICU bacterial community. The results showed that, although some bacterial populations decreased after cleaning, various HAI-related genera were quite stable following sanitization, suggesting being well-adapted to the ICU environment. Overall, these results enabled identification of discrete ICU and NICU reservoirs of potentially pathogenic bacteria and provided evidence for the presence of a set of biomarkers genera that distinguish these units. Moreover, the study exposed the inconsistencies of the routine cleaning to minimize HAI-related genera contamination.
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spelling pubmed-67245802019-09-25 Microbial Community Profiling in Intensive Care Units Expose Limitations in Current Sanitary Standards Ribeiro, Lucas Ferreira Lopes, Erica M. Kishi, Luciano T. Ribeiro, Liliane Fraga Costa Menegueti, Mayra Gonçalves Gaspar, Gilberto Gambero Silva-Rocha, Rafael Guazzaroni, María-Eugenia Front Public Health Public Health Hospital-associated infections (HAIs) are a leading cause of morbidity and mortality in intensive care units (ICUs) and neonatal intensive care units (NICUs). Organisms causing these infections are often present on surfaces around the patient. Given that microbiota may vary across different ICUs, the HAI-related microbial signatures within these units remain underexplored. In this study, we use deep-sequencing analyses to explore and compare the structure of bacterial communities at inanimate surfaces of the ICU and NICU wards of The Medical School Clinics Hospital (Brazil). The data revealed that NICU presents higher biodiversity than ICU and surfaces closest to the patient showed a peculiar microbiota, distinguishing one unit from the other. Several facultative anaerobes or obligate anaerobes HAI-related genera were classified as biomarkers for the NICU, whereas Pseudomonas was the main biomarker for ICU. Correlation analyses revealed a distinct pattern of microbe-microbe interactions for each unit, including bacteria able to form multi-genera biofilms. Furthermore, we evaluated the effect of concurrent cleaning over the ICU bacterial community. The results showed that, although some bacterial populations decreased after cleaning, various HAI-related genera were quite stable following sanitization, suggesting being well-adapted to the ICU environment. Overall, these results enabled identification of discrete ICU and NICU reservoirs of potentially pathogenic bacteria and provided evidence for the presence of a set of biomarkers genera that distinguish these units. Moreover, the study exposed the inconsistencies of the routine cleaning to minimize HAI-related genera contamination. Frontiers Media S.A. 2019-08-28 /pmc/articles/PMC6724580/ /pubmed/31555629 http://dx.doi.org/10.3389/fpubh.2019.00240 Text en Copyright © 2019 Ribeiro, Lopes, Kishi, Ribeiro, Menegueti, Gaspar, Silva-Rocha and Guazzaroni. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Public Health
Ribeiro, Lucas Ferreira
Lopes, Erica M.
Kishi, Luciano T.
Ribeiro, Liliane Fraga Costa
Menegueti, Mayra Gonçalves
Gaspar, Gilberto Gambero
Silva-Rocha, Rafael
Guazzaroni, María-Eugenia
Microbial Community Profiling in Intensive Care Units Expose Limitations in Current Sanitary Standards
title Microbial Community Profiling in Intensive Care Units Expose Limitations in Current Sanitary Standards
title_full Microbial Community Profiling in Intensive Care Units Expose Limitations in Current Sanitary Standards
title_fullStr Microbial Community Profiling in Intensive Care Units Expose Limitations in Current Sanitary Standards
title_full_unstemmed Microbial Community Profiling in Intensive Care Units Expose Limitations in Current Sanitary Standards
title_short Microbial Community Profiling in Intensive Care Units Expose Limitations in Current Sanitary Standards
title_sort microbial community profiling in intensive care units expose limitations in current sanitary standards
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724580/
https://www.ncbi.nlm.nih.gov/pubmed/31555629
http://dx.doi.org/10.3389/fpubh.2019.00240
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