Cargando…

A specialized condensin complex participates in somatic nuclear maturation in Tetrahymena thermophila

Condensins are highly conserved proteins that are important for chromosome maintenance in nearly all forms of life. Although many organisms employ two forms of the condensin complex, the condensin genes in Tetrahymena have expanded even further. Here we report a form of condensin that is specificall...

Descripción completa

Detalles Bibliográficos
Autores principales: Howard-Till, Rachel, Tian, Miao, Loidl, Josef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724606/
https://www.ncbi.nlm.nih.gov/pubmed/30893010
http://dx.doi.org/10.1091/mbc.E18-08-0487
_version_ 1783449026795929600
author Howard-Till, Rachel
Tian, Miao
Loidl, Josef
author_facet Howard-Till, Rachel
Tian, Miao
Loidl, Josef
author_sort Howard-Till, Rachel
collection PubMed
description Condensins are highly conserved proteins that are important for chromosome maintenance in nearly all forms of life. Although many organisms employ two forms of the condensin complex, the condensin genes in Tetrahymena have expanded even further. Here we report a form of condensin that is specifically active during sexual reproduction. This complex, condensin D, is composed of the core condensin proteins, Smc2 and Smc4, and two unique subunits, the kleisin Cph5 and Cpd2. Cpd2 is also found in somatic nuclei in vegetative cells, but is dispensable for growth and nuclear division. Immunoprecipitation experiments show that condensin D interacts with a putative member of a chromatin-remodeling complex during development. Condensin D is required for sexual reproduction and for endoreplication and genome reduction of the progeny’s somatic nuclei. Altogether, Tetrahymena possesses at least four forms of condensin to fulfill the needs of maintaining chromosomes in two different nuclei containing the somatic and germline genomes.
format Online
Article
Text
id pubmed-6724606
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-67246062019-09-06 A specialized condensin complex participates in somatic nuclear maturation in Tetrahymena thermophila Howard-Till, Rachel Tian, Miao Loidl, Josef Mol Biol Cell Articles Condensins are highly conserved proteins that are important for chromosome maintenance in nearly all forms of life. Although many organisms employ two forms of the condensin complex, the condensin genes in Tetrahymena have expanded even further. Here we report a form of condensin that is specifically active during sexual reproduction. This complex, condensin D, is composed of the core condensin proteins, Smc2 and Smc4, and two unique subunits, the kleisin Cph5 and Cpd2. Cpd2 is also found in somatic nuclei in vegetative cells, but is dispensable for growth and nuclear division. Immunoprecipitation experiments show that condensin D interacts with a putative member of a chromatin-remodeling complex during development. Condensin D is required for sexual reproduction and for endoreplication and genome reduction of the progeny’s somatic nuclei. Altogether, Tetrahymena possesses at least four forms of condensin to fulfill the needs of maintaining chromosomes in two different nuclei containing the somatic and germline genomes. The American Society for Cell Biology 2019-05-15 /pmc/articles/PMC6724606/ /pubmed/30893010 http://dx.doi.org/10.1091/mbc.E18-08-0487 Text en © 2019 Howard-Till et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Howard-Till, Rachel
Tian, Miao
Loidl, Josef
A specialized condensin complex participates in somatic nuclear maturation in Tetrahymena thermophila
title A specialized condensin complex participates in somatic nuclear maturation in Tetrahymena thermophila
title_full A specialized condensin complex participates in somatic nuclear maturation in Tetrahymena thermophila
title_fullStr A specialized condensin complex participates in somatic nuclear maturation in Tetrahymena thermophila
title_full_unstemmed A specialized condensin complex participates in somatic nuclear maturation in Tetrahymena thermophila
title_short A specialized condensin complex participates in somatic nuclear maturation in Tetrahymena thermophila
title_sort specialized condensin complex participates in somatic nuclear maturation in tetrahymena thermophila
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6724606/
https://www.ncbi.nlm.nih.gov/pubmed/30893010
http://dx.doi.org/10.1091/mbc.E18-08-0487
work_keys_str_mv AT howardtillrachel aspecializedcondensincomplexparticipatesinsomaticnuclearmaturationintetrahymenathermophila
AT tianmiao aspecializedcondensincomplexparticipatesinsomaticnuclearmaturationintetrahymenathermophila
AT loidljosef aspecializedcondensincomplexparticipatesinsomaticnuclearmaturationintetrahymenathermophila
AT howardtillrachel specializedcondensincomplexparticipatesinsomaticnuclearmaturationintetrahymenathermophila
AT tianmiao specializedcondensincomplexparticipatesinsomaticnuclearmaturationintetrahymenathermophila
AT loidljosef specializedcondensincomplexparticipatesinsomaticnuclearmaturationintetrahymenathermophila