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The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility
The ubiquitin proteasome system regulates meiotic recombination in yeast through its association with the synaptonemal complex, a ‘zipper’-like structure that holds homologous chromosome pairs in synapsis during meiotic prophase I. In mammals, the proteasome activator subunit PA200 targets acetylate...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726247/ https://www.ncbi.nlm.nih.gov/pubmed/31437213 http://dx.doi.org/10.1371/journal.pgen.1008316 |
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author | Gómez-H, Laura Felipe-Medina, Natalia Condezo, Yazmine B. Garcia-Valiente, Rodrigo Ramos, Isabel Suja, José Angel Barbero, José Luis Roig, Ignasi Sánchez-Martín, Manuel de Rooij, Dirk G. Llano, Elena Pendas, Alberto M. |
author_facet | Gómez-H, Laura Felipe-Medina, Natalia Condezo, Yazmine B. Garcia-Valiente, Rodrigo Ramos, Isabel Suja, José Angel Barbero, José Luis Roig, Ignasi Sánchez-Martín, Manuel de Rooij, Dirk G. Llano, Elena Pendas, Alberto M. |
author_sort | Gómez-H, Laura |
collection | PubMed |
description | The ubiquitin proteasome system regulates meiotic recombination in yeast through its association with the synaptonemal complex, a ‘zipper’-like structure that holds homologous chromosome pairs in synapsis during meiotic prophase I. In mammals, the proteasome activator subunit PA200 targets acetylated histones for degradation during somatic DNA double strand break repair and during histone replacement during spermiogenesis. We investigated the role of the testis-specific proteasomal subunit α4s (PSMA8) during spermatogenesis, and found that PSMA8 was localized to and dependent on the central region of the synaptonemal complex. Accordingly, synapsis-deficient mice show delocalization of PSMA8. Moreover, though Psma8-deficient mice are proficient in meiotic homologous recombination, there are alterations in the proteostasis of several key meiotic players that, in addition to the known substrate acetylated histones, have been shown by a proteomic approach to interact with PSMA8, such as SYCP3, SYCP1, CDK1 and TRIP13. These alterations lead to an accumulation of spermatocytes in metaphase I and II which either enter massively into apoptosis or give rise to a low number of aberrant round spermatids that apoptose before histone replacement takes place. |
format | Online Article Text |
id | pubmed-6726247 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-67262472019-09-10 The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility Gómez-H, Laura Felipe-Medina, Natalia Condezo, Yazmine B. Garcia-Valiente, Rodrigo Ramos, Isabel Suja, José Angel Barbero, José Luis Roig, Ignasi Sánchez-Martín, Manuel de Rooij, Dirk G. Llano, Elena Pendas, Alberto M. PLoS Genet Research Article The ubiquitin proteasome system regulates meiotic recombination in yeast through its association with the synaptonemal complex, a ‘zipper’-like structure that holds homologous chromosome pairs in synapsis during meiotic prophase I. In mammals, the proteasome activator subunit PA200 targets acetylated histones for degradation during somatic DNA double strand break repair and during histone replacement during spermiogenesis. We investigated the role of the testis-specific proteasomal subunit α4s (PSMA8) during spermatogenesis, and found that PSMA8 was localized to and dependent on the central region of the synaptonemal complex. Accordingly, synapsis-deficient mice show delocalization of PSMA8. Moreover, though Psma8-deficient mice are proficient in meiotic homologous recombination, there are alterations in the proteostasis of several key meiotic players that, in addition to the known substrate acetylated histones, have been shown by a proteomic approach to interact with PSMA8, such as SYCP3, SYCP1, CDK1 and TRIP13. These alterations lead to an accumulation of spermatocytes in metaphase I and II which either enter massively into apoptosis or give rise to a low number of aberrant round spermatids that apoptose before histone replacement takes place. Public Library of Science 2019-08-22 /pmc/articles/PMC6726247/ /pubmed/31437213 http://dx.doi.org/10.1371/journal.pgen.1008316 Text en © 2019 Gómez-H et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Gómez-H, Laura Felipe-Medina, Natalia Condezo, Yazmine B. Garcia-Valiente, Rodrigo Ramos, Isabel Suja, José Angel Barbero, José Luis Roig, Ignasi Sánchez-Martín, Manuel de Rooij, Dirk G. Llano, Elena Pendas, Alberto M. The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility |
title | The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility |
title_full | The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility |
title_fullStr | The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility |
title_full_unstemmed | The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility |
title_short | The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility |
title_sort | psma8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726247/ https://www.ncbi.nlm.nih.gov/pubmed/31437213 http://dx.doi.org/10.1371/journal.pgen.1008316 |
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