Cargando…
The impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system
This study was conducted to assess the effects of addition of 10% newborn bovine serum (NBS) in the maturation and culture media of bovine system on oocytes maturation and developed morula and blastocyst rates. For this purpose, 10% NBS was added to in vitro maturation (IVM) medium alone (Experiment...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726305/ https://www.ncbi.nlm.nih.gov/pubmed/31595239 http://dx.doi.org/10.1016/j.pbj.0000000000000003 |
_version_ | 1783449077027962880 |
---|---|
author | Abdel-Wahab, Ahmed Abdel Aziz, Rabie L. Helmy, Nermeen A. Ibrahim, Shawky S. |
author_facet | Abdel-Wahab, Ahmed Abdel Aziz, Rabie L. Helmy, Nermeen A. Ibrahim, Shawky S. |
author_sort | Abdel-Wahab, Ahmed |
collection | PubMed |
description | This study was conducted to assess the effects of addition of 10% newborn bovine serum (NBS) in the maturation and culture media of bovine system on oocytes maturation and developed morula and blastocyst rates. For this purpose, 10% NBS was added to in vitro maturation (IVM) medium alone (Experiment I), in vitro culture (IVC) (Experiment II), and in IVM + IVC (Experiment III). In vitro bovine oocytes maturation and developmental rates of bovine embryos were then compared with control group where the basal maturation or culture media were supplemented with 10% fetal calf serum (FCS). Results revealed that maturation, cleavage, morula, and blastocyst formation rates of in vitro fertilized embryos were not significantly different in NBS and control groups in the 3 experiments. In conclusion, NBS is an efficient macromolecule in bovine system when added either to IVM or to IVC or to both stages. Thus, NBS could provide cheaper and more available treatment to be used as FCS substitute in the IVC system of bovines. |
format | Online Article Text |
id | pubmed-6726305 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
record_format | MEDLINE/PubMed |
spelling | pubmed-67263052019-10-08 The impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system Abdel-Wahab, Ahmed Abdel Aziz, Rabie L. Helmy, Nermeen A. Ibrahim, Shawky S. Porto Biomed J Original Article This study was conducted to assess the effects of addition of 10% newborn bovine serum (NBS) in the maturation and culture media of bovine system on oocytes maturation and developed morula and blastocyst rates. For this purpose, 10% NBS was added to in vitro maturation (IVM) medium alone (Experiment I), in vitro culture (IVC) (Experiment II), and in IVM + IVC (Experiment III). In vitro bovine oocytes maturation and developmental rates of bovine embryos were then compared with control group where the basal maturation or culture media were supplemented with 10% fetal calf serum (FCS). Results revealed that maturation, cleavage, morula, and blastocyst formation rates of in vitro fertilized embryos were not significantly different in NBS and control groups in the 3 experiments. In conclusion, NBS is an efficient macromolecule in bovine system when added either to IVM or to IVC or to both stages. Thus, NBS could provide cheaper and more available treatment to be used as FCS substitute in the IVC system of bovines. 2018-07-03 /pmc/articles/PMC6726305/ /pubmed/31595239 http://dx.doi.org/10.1016/j.pbj.0000000000000003 Text en Copyright © 2018 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of PBJ-Associação Porto Biomedical/Porto Biomedical Society. All rights reserved. http://creativecommons.org/licenses/by-nc-nd/4.0 This-is-an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0 |
spellingShingle | Original Article Abdel-Wahab, Ahmed Abdel Aziz, Rabie L. Helmy, Nermeen A. Ibrahim, Shawky S. The impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system |
title | The impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system |
title_full | The impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system |
title_fullStr | The impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system |
title_full_unstemmed | The impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system |
title_short | The impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system |
title_sort | impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726305/ https://www.ncbi.nlm.nih.gov/pubmed/31595239 http://dx.doi.org/10.1016/j.pbj.0000000000000003 |
work_keys_str_mv | AT abdelwahabahmed theimpactofusingnewbornbovineserumasfetalcalfserumsubstituteintheinvitrobovineembryosproductionsystem AT abdelazizrabiel theimpactofusingnewbornbovineserumasfetalcalfserumsubstituteintheinvitrobovineembryosproductionsystem AT helmynermeena theimpactofusingnewbornbovineserumasfetalcalfserumsubstituteintheinvitrobovineembryosproductionsystem AT ibrahimshawkys theimpactofusingnewbornbovineserumasfetalcalfserumsubstituteintheinvitrobovineembryosproductionsystem AT abdelwahabahmed impactofusingnewbornbovineserumasfetalcalfserumsubstituteintheinvitrobovineembryosproductionsystem AT abdelazizrabiel impactofusingnewbornbovineserumasfetalcalfserumsubstituteintheinvitrobovineembryosproductionsystem AT helmynermeena impactofusingnewbornbovineserumasfetalcalfserumsubstituteintheinvitrobovineembryosproductionsystem AT ibrahimshawkys impactofusingnewbornbovineserumasfetalcalfserumsubstituteintheinvitrobovineembryosproductionsystem |