Cargando…
Inducing Mild Traumatic Brain Injury in C. elegans via Cavitation-Free Surface Acoustic Wave-Driven Ultrasonic Irradiation
Mild traumatic brain injury is an all-too-common outcome from modern warfare and sport, and lacks a reproducible model for assessment of potential treatments and protection against it. Here we consider the use of surface acoustic wave (SAW) irradiation of C. elegans worms—without cavitation—as a pot...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726767/ https://www.ncbi.nlm.nih.gov/pubmed/31485018 http://dx.doi.org/10.1038/s41598-019-47295-1 |
_version_ | 1783449147153580032 |
---|---|
author | Miansari, Morteza Mehta, Meghna D. Schilling, Jan M. Kurashina, Yuta Patel, Hemal H. Friend, James |
author_facet | Miansari, Morteza Mehta, Meghna D. Schilling, Jan M. Kurashina, Yuta Patel, Hemal H. Friend, James |
author_sort | Miansari, Morteza |
collection | PubMed |
description | Mild traumatic brain injury is an all-too-common outcome from modern warfare and sport, and lacks a reproducible model for assessment of potential treatments and protection against it. Here we consider the use of surface acoustic wave (SAW) irradiation of C. elegans worms—without cavitation—as a potential, ethically reasonable animal-on-a-chip model for inducing traumatic brain injury in an animal, producing significant effects on memory and learning that could prove useful in a model that progress from youth to old age in but a few weeks. We show a significant effect by SAW on the ability of worms to learn post-exposure through associative learning chemotaxis. At higher SAW intensity, we find immediate, thorough, but temporary paralysis of the worms. We further explore the importance of homogeneous exposure of the worms to the SAW-driven ultrasound, an aspect poorly controlled in past efforts, if at all, and demonstrate the absence of cavitation through a change in fluids from a standard media for the worms to the exceedingly viscous polyvinyl alcohol. Likewise, we demonstrate that acoustic streaming, when present, is not directly responsible for paralysis nor learning disabilities induced in the worm, but is beneficial at low amplitudes to ensuring homogeneous ultrasound exposure. |
format | Online Article Text |
id | pubmed-6726767 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67267672019-09-18 Inducing Mild Traumatic Brain Injury in C. elegans via Cavitation-Free Surface Acoustic Wave-Driven Ultrasonic Irradiation Miansari, Morteza Mehta, Meghna D. Schilling, Jan M. Kurashina, Yuta Patel, Hemal H. Friend, James Sci Rep Article Mild traumatic brain injury is an all-too-common outcome from modern warfare and sport, and lacks a reproducible model for assessment of potential treatments and protection against it. Here we consider the use of surface acoustic wave (SAW) irradiation of C. elegans worms—without cavitation—as a potential, ethically reasonable animal-on-a-chip model for inducing traumatic brain injury in an animal, producing significant effects on memory and learning that could prove useful in a model that progress from youth to old age in but a few weeks. We show a significant effect by SAW on the ability of worms to learn post-exposure through associative learning chemotaxis. At higher SAW intensity, we find immediate, thorough, but temporary paralysis of the worms. We further explore the importance of homogeneous exposure of the worms to the SAW-driven ultrasound, an aspect poorly controlled in past efforts, if at all, and demonstrate the absence of cavitation through a change in fluids from a standard media for the worms to the exceedingly viscous polyvinyl alcohol. Likewise, we demonstrate that acoustic streaming, when present, is not directly responsible for paralysis nor learning disabilities induced in the worm, but is beneficial at low amplitudes to ensuring homogeneous ultrasound exposure. Nature Publishing Group UK 2019-09-04 /pmc/articles/PMC6726767/ /pubmed/31485018 http://dx.doi.org/10.1038/s41598-019-47295-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Miansari, Morteza Mehta, Meghna D. Schilling, Jan M. Kurashina, Yuta Patel, Hemal H. Friend, James Inducing Mild Traumatic Brain Injury in C. elegans via Cavitation-Free Surface Acoustic Wave-Driven Ultrasonic Irradiation |
title | Inducing Mild Traumatic Brain Injury in C. elegans via Cavitation-Free Surface Acoustic Wave-Driven Ultrasonic Irradiation |
title_full | Inducing Mild Traumatic Brain Injury in C. elegans via Cavitation-Free Surface Acoustic Wave-Driven Ultrasonic Irradiation |
title_fullStr | Inducing Mild Traumatic Brain Injury in C. elegans via Cavitation-Free Surface Acoustic Wave-Driven Ultrasonic Irradiation |
title_full_unstemmed | Inducing Mild Traumatic Brain Injury in C. elegans via Cavitation-Free Surface Acoustic Wave-Driven Ultrasonic Irradiation |
title_short | Inducing Mild Traumatic Brain Injury in C. elegans via Cavitation-Free Surface Acoustic Wave-Driven Ultrasonic Irradiation |
title_sort | inducing mild traumatic brain injury in c. elegans via cavitation-free surface acoustic wave-driven ultrasonic irradiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726767/ https://www.ncbi.nlm.nih.gov/pubmed/31485018 http://dx.doi.org/10.1038/s41598-019-47295-1 |
work_keys_str_mv | AT miansarimorteza inducingmildtraumaticbraininjuryincelegansviacavitationfreesurfaceacousticwavedrivenultrasonicirradiation AT mehtameghnad inducingmildtraumaticbraininjuryincelegansviacavitationfreesurfaceacousticwavedrivenultrasonicirradiation AT schillingjanm inducingmildtraumaticbraininjuryincelegansviacavitationfreesurfaceacousticwavedrivenultrasonicirradiation AT kurashinayuta inducingmildtraumaticbraininjuryincelegansviacavitationfreesurfaceacousticwavedrivenultrasonicirradiation AT patelhemalh inducingmildtraumaticbraininjuryincelegansviacavitationfreesurfaceacousticwavedrivenultrasonicirradiation AT friendjames inducingmildtraumaticbraininjuryincelegansviacavitationfreesurfaceacousticwavedrivenultrasonicirradiation |