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LncRNA PAPAS may promote triple-negative breast cancer by downregulating miR-34a

OBJECTIVE: To investigate the role of promoter and pre-rRNA antisense (PAPAS) long noncoding (Lnc) RNA in cancer biology. METHODS: Tumour and tumour-adjacent healthy tissue biopsies from patients with triple-negative breast cancer (TNBC), and plasma samples from these patients plus healthy controls,...

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Autores principales: Kong, Yan, Geng, Cuizhi, Dong, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726796/
https://www.ncbi.nlm.nih.gov/pubmed/31248310
http://dx.doi.org/10.1177/0300060519850724
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author Kong, Yan
Geng, Cuizhi
Dong, Qian
author_facet Kong, Yan
Geng, Cuizhi
Dong, Qian
author_sort Kong, Yan
collection PubMed
description OBJECTIVE: To investigate the role of promoter and pre-rRNA antisense (PAPAS) long noncoding (Lnc) RNA in cancer biology. METHODS: Tumour and tumour-adjacent healthy tissue biopsies from patients with triple-negative breast cancer (TNBC), and plasma samples from these patients plus healthy controls, were assessed for PAPAS and microRNA (miR)-34a. Effects of PAPAS and miR-34a overexpression were also investigated in vitro. RESULTS: PAPAS was upregulated in tumour tissues of patients with TNBC versus tumour-adjacent healthy tissues. Plasma PAPAS levels were also upregulated in patients with TNBC versus healthy controls. Levels of PAPAS in tumour tissue was significantly positively correlated with PAPAS levels in plasma from patients with TNBC. MiR-34a was downregulated in tumour tissues versus adjacent healthy tissues, and was significantly correlated with PAPAS in tumour tissues. PAPAS overexpression in vitro was associated with miR-34a inhibition, while miR-34a failed to significantly affect PAPAS levels. PAPAS overexpression promoted in vitro migration and invasion of TNBC cells, while miR-34a overexpression was inhibitory. MiR-34a overexpression decreased the enhanced cell migration and invasion associated with PAPAS overexpression. PAPAS overexpression showed no significant effects on cancer-cell proliferation. CONCLUSION: LncRNA PAPAS may promote TNBC by downregulating miR-34a.
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spelling pubmed-67267962019-09-13 LncRNA PAPAS may promote triple-negative breast cancer by downregulating miR-34a Kong, Yan Geng, Cuizhi Dong, Qian J Int Med Res Clinical Research Reports OBJECTIVE: To investigate the role of promoter and pre-rRNA antisense (PAPAS) long noncoding (Lnc) RNA in cancer biology. METHODS: Tumour and tumour-adjacent healthy tissue biopsies from patients with triple-negative breast cancer (TNBC), and plasma samples from these patients plus healthy controls, were assessed for PAPAS and microRNA (miR)-34a. Effects of PAPAS and miR-34a overexpression were also investigated in vitro. RESULTS: PAPAS was upregulated in tumour tissues of patients with TNBC versus tumour-adjacent healthy tissues. Plasma PAPAS levels were also upregulated in patients with TNBC versus healthy controls. Levels of PAPAS in tumour tissue was significantly positively correlated with PAPAS levels in plasma from patients with TNBC. MiR-34a was downregulated in tumour tissues versus adjacent healthy tissues, and was significantly correlated with PAPAS in tumour tissues. PAPAS overexpression in vitro was associated with miR-34a inhibition, while miR-34a failed to significantly affect PAPAS levels. PAPAS overexpression promoted in vitro migration and invasion of TNBC cells, while miR-34a overexpression was inhibitory. MiR-34a overexpression decreased the enhanced cell migration and invasion associated with PAPAS overexpression. PAPAS overexpression showed no significant effects on cancer-cell proliferation. CONCLUSION: LncRNA PAPAS may promote TNBC by downregulating miR-34a. SAGE Publications 2019-06-27 2019-08 /pmc/articles/PMC6726796/ /pubmed/31248310 http://dx.doi.org/10.1177/0300060519850724 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Clinical Research Reports
Kong, Yan
Geng, Cuizhi
Dong, Qian
LncRNA PAPAS may promote triple-negative breast cancer by downregulating miR-34a
title LncRNA PAPAS may promote triple-negative breast cancer by downregulating miR-34a
title_full LncRNA PAPAS may promote triple-negative breast cancer by downregulating miR-34a
title_fullStr LncRNA PAPAS may promote triple-negative breast cancer by downregulating miR-34a
title_full_unstemmed LncRNA PAPAS may promote triple-negative breast cancer by downregulating miR-34a
title_short LncRNA PAPAS may promote triple-negative breast cancer by downregulating miR-34a
title_sort lncrna papas may promote triple-negative breast cancer by downregulating mir-34a
topic Clinical Research Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726796/
https://www.ncbi.nlm.nih.gov/pubmed/31248310
http://dx.doi.org/10.1177/0300060519850724
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