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Distribution of Capsular Types of Campylobacter jejuni Isolates from Symptomatic and Asymptomatic Children in Peru
Campylobacter jejuni is the leading bacterial cause of diarrhea worldwide. A capsular polysaccharide (CPS) conjugate vaccine is under development and requires determination of the valency. However, distribution of CPS types circulating globally is presently poorly described. We aimed to determine wh...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The American Society of Tropical Medicine and Hygiene
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726929/ https://www.ncbi.nlm.nih.gov/pubmed/31392944 http://dx.doi.org/10.4269/ajtmh.18-0994 |
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author | Rojas, Jesús D. Reynolds, Nathanael D. Pike, Brian L. Espinoza, Nereyda M. Kuroiwa, Janelle Jani, Vihasi Ríos, Paul A. Nunez, Rosa G. Yori, Pablo P. Bernal, Manuela Meza, Rina A. Kosek, Margaret N. McCoy, Andrea J. Simons, Mark P. Gregory, Michael J. Guerry, Patricia Poly, Frédéric M. |
author_facet | Rojas, Jesús D. Reynolds, Nathanael D. Pike, Brian L. Espinoza, Nereyda M. Kuroiwa, Janelle Jani, Vihasi Ríos, Paul A. Nunez, Rosa G. Yori, Pablo P. Bernal, Manuela Meza, Rina A. Kosek, Margaret N. McCoy, Andrea J. Simons, Mark P. Gregory, Michael J. Guerry, Patricia Poly, Frédéric M. |
author_sort | Rojas, Jesús D. |
collection | PubMed |
description | Campylobacter jejuni is the leading bacterial cause of diarrhea worldwide. A capsular polysaccharide (CPS) conjugate vaccine is under development and requires determination of the valency. However, distribution of CPS types circulating globally is presently poorly described. We aimed to determine whether CPS type distribution in Peru differs from that in other endemic regions. We used a multiplex polymerase chain reaction (PCR) assay for the detection of CPS encoding genes capable of distinguishing all 35 CPS types on Campylobacter isolates in two prospective communities based studies conducted in cohorts of children less than 59 months of age in Peru. Results showed that CPS type HS4 complex was the most prevalent, followed by HS3 complex and HS15. Differences in CPS type for symptomatology were not statistically significant. Most subjects demonstrated repeated infections over time with different CPS types, suggesting that CPS types may confer of a level of homologous protective immunity. In this dataset, some differences in CPS type distribution were observed in comparison to other low-middle income countries. Further studies need to be conducted in endemic areas to increase our knowledge of CPS type distribution and guide vaccine development. |
format | Online Article Text |
id | pubmed-6726929 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The American Society of Tropical Medicine and Hygiene |
record_format | MEDLINE/PubMed |
spelling | pubmed-67269292019-09-08 Distribution of Capsular Types of Campylobacter jejuni Isolates from Symptomatic and Asymptomatic Children in Peru Rojas, Jesús D. Reynolds, Nathanael D. Pike, Brian L. Espinoza, Nereyda M. Kuroiwa, Janelle Jani, Vihasi Ríos, Paul A. Nunez, Rosa G. Yori, Pablo P. Bernal, Manuela Meza, Rina A. Kosek, Margaret N. McCoy, Andrea J. Simons, Mark P. Gregory, Michael J. Guerry, Patricia Poly, Frédéric M. Am J Trop Med Hyg Articles Campylobacter jejuni is the leading bacterial cause of diarrhea worldwide. A capsular polysaccharide (CPS) conjugate vaccine is under development and requires determination of the valency. However, distribution of CPS types circulating globally is presently poorly described. We aimed to determine whether CPS type distribution in Peru differs from that in other endemic regions. We used a multiplex polymerase chain reaction (PCR) assay for the detection of CPS encoding genes capable of distinguishing all 35 CPS types on Campylobacter isolates in two prospective communities based studies conducted in cohorts of children less than 59 months of age in Peru. Results showed that CPS type HS4 complex was the most prevalent, followed by HS3 complex and HS15. Differences in CPS type for symptomatology were not statistically significant. Most subjects demonstrated repeated infections over time with different CPS types, suggesting that CPS types may confer of a level of homologous protective immunity. In this dataset, some differences in CPS type distribution were observed in comparison to other low-middle income countries. Further studies need to be conducted in endemic areas to increase our knowledge of CPS type distribution and guide vaccine development. The American Society of Tropical Medicine and Hygiene 2019-09 2019-08-05 /pmc/articles/PMC6726929/ /pubmed/31392944 http://dx.doi.org/10.4269/ajtmh.18-0994 Text en © The American Society of Tropical Medicine and Hygiene This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Articles Rojas, Jesús D. Reynolds, Nathanael D. Pike, Brian L. Espinoza, Nereyda M. Kuroiwa, Janelle Jani, Vihasi Ríos, Paul A. Nunez, Rosa G. Yori, Pablo P. Bernal, Manuela Meza, Rina A. Kosek, Margaret N. McCoy, Andrea J. Simons, Mark P. Gregory, Michael J. Guerry, Patricia Poly, Frédéric M. Distribution of Capsular Types of Campylobacter jejuni Isolates from Symptomatic and Asymptomatic Children in Peru |
title | Distribution of Capsular Types of Campylobacter jejuni Isolates from Symptomatic and Asymptomatic Children in Peru |
title_full | Distribution of Capsular Types of Campylobacter jejuni Isolates from Symptomatic and Asymptomatic Children in Peru |
title_fullStr | Distribution of Capsular Types of Campylobacter jejuni Isolates from Symptomatic and Asymptomatic Children in Peru |
title_full_unstemmed | Distribution of Capsular Types of Campylobacter jejuni Isolates from Symptomatic and Asymptomatic Children in Peru |
title_short | Distribution of Capsular Types of Campylobacter jejuni Isolates from Symptomatic and Asymptomatic Children in Peru |
title_sort | distribution of capsular types of campylobacter jejuni isolates from symptomatic and asymptomatic children in peru |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6726929/ https://www.ncbi.nlm.nih.gov/pubmed/31392944 http://dx.doi.org/10.4269/ajtmh.18-0994 |
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