Ex vivo electrochemical measurement of glutamate release during spinal cord injury

Excessive glutamate release following traumatic spinal cord injury (SCI) has been associated with exacerbating the extent of SCI. However, the mechanism behind sustained high levels of extracellular glutamate is unclear. Spinal cord segments mounted in a sucrose double gap recording chamber are an e...

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Autores principales: Nolan, James K., Nguyen, Tran N.H., Fattah, Mara, Page, Jessica C., Shi, Riyi, Lee, Hyowon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727010/
https://www.ncbi.nlm.nih.gov/pubmed/31508326
http://dx.doi.org/10.1016/j.mex.2019.08.008
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author Nolan, James K.
Nguyen, Tran N.H.
Fattah, Mara
Page, Jessica C.
Shi, Riyi
Lee, Hyowon
author_facet Nolan, James K.
Nguyen, Tran N.H.
Fattah, Mara
Page, Jessica C.
Shi, Riyi
Lee, Hyowon
author_sort Nolan, James K.
collection PubMed
description Excessive glutamate release following traumatic spinal cord injury (SCI) has been associated with exacerbating the extent of SCI. However, the mechanism behind sustained high levels of extracellular glutamate is unclear. Spinal cord segments mounted in a sucrose double gap recording chamber are an established model for traumatic spinal cord injury. We have developed a method to record, with micro-scale printed glutamate biosensors, glutamate release from ex vivo rat spinal cord segments following injury. This protocol would work equally well for similar glutamate biosensors.
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spelling pubmed-67270102019-09-10 Ex vivo electrochemical measurement of glutamate release during spinal cord injury Nolan, James K. Nguyen, Tran N.H. Fattah, Mara Page, Jessica C. Shi, Riyi Lee, Hyowon MethodsX Neuroscience Excessive glutamate release following traumatic spinal cord injury (SCI) has been associated with exacerbating the extent of SCI. However, the mechanism behind sustained high levels of extracellular glutamate is unclear. Spinal cord segments mounted in a sucrose double gap recording chamber are an established model for traumatic spinal cord injury. We have developed a method to record, with micro-scale printed glutamate biosensors, glutamate release from ex vivo rat spinal cord segments following injury. This protocol would work equally well for similar glutamate biosensors. Elsevier 2019-08-23 /pmc/articles/PMC6727010/ /pubmed/31508326 http://dx.doi.org/10.1016/j.mex.2019.08.008 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Neuroscience
Nolan, James K.
Nguyen, Tran N.H.
Fattah, Mara
Page, Jessica C.
Shi, Riyi
Lee, Hyowon
Ex vivo electrochemical measurement of glutamate release during spinal cord injury
title Ex vivo electrochemical measurement of glutamate release during spinal cord injury
title_full Ex vivo electrochemical measurement of glutamate release during spinal cord injury
title_fullStr Ex vivo electrochemical measurement of glutamate release during spinal cord injury
title_full_unstemmed Ex vivo electrochemical measurement of glutamate release during spinal cord injury
title_short Ex vivo electrochemical measurement of glutamate release during spinal cord injury
title_sort ex vivo electrochemical measurement of glutamate release during spinal cord injury
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727010/
https://www.ncbi.nlm.nih.gov/pubmed/31508326
http://dx.doi.org/10.1016/j.mex.2019.08.008
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