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Pinpointing Morphology and Projection of Excitatory Neurons in Mouse Visual Cortex

The excitatory neurons in the visual cortex are of great significance for us in understanding brain functions. However, the diverse neuron types and their morphological properties have not been fully deciphered. In this paper, we applied the brain-wide positioning system (BPS) to image the entire br...

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Autores principales: Zhang, Yalun, Jiang, Siqi, Xu, Zhengchao, Gong, Hui, Li, Anan, Luo, Qingming, Ren, Miao, Li, Xiangning, Wu, Hao, Yuan, Jing, Chen, Shangbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727359/
https://www.ncbi.nlm.nih.gov/pubmed/31555081
http://dx.doi.org/10.3389/fnins.2019.00912
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author Zhang, Yalun
Jiang, Siqi
Xu, Zhengchao
Gong, Hui
Li, Anan
Luo, Qingming
Ren, Miao
Li, Xiangning
Wu, Hao
Yuan, Jing
Chen, Shangbin
author_facet Zhang, Yalun
Jiang, Siqi
Xu, Zhengchao
Gong, Hui
Li, Anan
Luo, Qingming
Ren, Miao
Li, Xiangning
Wu, Hao
Yuan, Jing
Chen, Shangbin
author_sort Zhang, Yalun
collection PubMed
description The excitatory neurons in the visual cortex are of great significance for us in understanding brain functions. However, the diverse neuron types and their morphological properties have not been fully deciphered. In this paper, we applied the brain-wide positioning system (BPS) to image the entire brain of two Thy1-eYFP H-line male mice at 0.2 μm × 0.2 μm × 1 μm voxel resolution. A total of 103 neurons were reconstructed in layers 5 and 6 of the visual cortex with single-axon-level resolution. Based on the complete topology of neurons and the inherent positioning function of the imaging method, we classified the observed neurons into six types according to their apical dendrites and somata location: star pyramidal cells in layer 5 (L5-sp), slender-tufted pyramidal cells in layer 5 (L5-st), tufted pyramidal cells in layer 5 (L5-tt), spiny stellate-like cells in layer 6 (L6-ss), star pyramidal cells in layer 6 (L6-sp), and slender-tufted pyramidal cells in layer 6 (L6-st). By examining the axonal projection patterns of individual neurons, they can be categorized into three modes: ipsilateral circuit connection neurons, callosal projection neurons and corticofugal projection neurons. Correlating the two types of classifications, we have found that there are at least two projection modes comprised in the former defined neuron types except for L5-tt. On the other hand, each projection mode may consist of four dendritic types defined in this study. The axon projection mode only partially correlates with the apical dendrite feature. This work has demonstrated a paradigm for resolving the visual cortex through single-neuron-level quantification and has shown potential to be extended to reveal the connectome of other defined sensory and motor systems.
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spelling pubmed-67273592019-09-25 Pinpointing Morphology and Projection of Excitatory Neurons in Mouse Visual Cortex Zhang, Yalun Jiang, Siqi Xu, Zhengchao Gong, Hui Li, Anan Luo, Qingming Ren, Miao Li, Xiangning Wu, Hao Yuan, Jing Chen, Shangbin Front Neurosci Neuroscience The excitatory neurons in the visual cortex are of great significance for us in understanding brain functions. However, the diverse neuron types and their morphological properties have not been fully deciphered. In this paper, we applied the brain-wide positioning system (BPS) to image the entire brain of two Thy1-eYFP H-line male mice at 0.2 μm × 0.2 μm × 1 μm voxel resolution. A total of 103 neurons were reconstructed in layers 5 and 6 of the visual cortex with single-axon-level resolution. Based on the complete topology of neurons and the inherent positioning function of the imaging method, we classified the observed neurons into six types according to their apical dendrites and somata location: star pyramidal cells in layer 5 (L5-sp), slender-tufted pyramidal cells in layer 5 (L5-st), tufted pyramidal cells in layer 5 (L5-tt), spiny stellate-like cells in layer 6 (L6-ss), star pyramidal cells in layer 6 (L6-sp), and slender-tufted pyramidal cells in layer 6 (L6-st). By examining the axonal projection patterns of individual neurons, they can be categorized into three modes: ipsilateral circuit connection neurons, callosal projection neurons and corticofugal projection neurons. Correlating the two types of classifications, we have found that there are at least two projection modes comprised in the former defined neuron types except for L5-tt. On the other hand, each projection mode may consist of four dendritic types defined in this study. The axon projection mode only partially correlates with the apical dendrite feature. This work has demonstrated a paradigm for resolving the visual cortex through single-neuron-level quantification and has shown potential to be extended to reveal the connectome of other defined sensory and motor systems. Frontiers Media S.A. 2019-08-29 /pmc/articles/PMC6727359/ /pubmed/31555081 http://dx.doi.org/10.3389/fnins.2019.00912 Text en Copyright © 2019 Zhang, Jiang, Xu, Gong, Li, Luo, Ren, Li, Wu, Yuan and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Zhang, Yalun
Jiang, Siqi
Xu, Zhengchao
Gong, Hui
Li, Anan
Luo, Qingming
Ren, Miao
Li, Xiangning
Wu, Hao
Yuan, Jing
Chen, Shangbin
Pinpointing Morphology and Projection of Excitatory Neurons in Mouse Visual Cortex
title Pinpointing Morphology and Projection of Excitatory Neurons in Mouse Visual Cortex
title_full Pinpointing Morphology and Projection of Excitatory Neurons in Mouse Visual Cortex
title_fullStr Pinpointing Morphology and Projection of Excitatory Neurons in Mouse Visual Cortex
title_full_unstemmed Pinpointing Morphology and Projection of Excitatory Neurons in Mouse Visual Cortex
title_short Pinpointing Morphology and Projection of Excitatory Neurons in Mouse Visual Cortex
title_sort pinpointing morphology and projection of excitatory neurons in mouse visual cortex
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727359/
https://www.ncbi.nlm.nih.gov/pubmed/31555081
http://dx.doi.org/10.3389/fnins.2019.00912
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