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Altered Expression of Complement Regulatory Proteins CD35, CD46, CD55, and CD59 on Leukocyte Subsets in Individuals Suffering From Coronary Artery Disease

Studies conducted in animal models have suggested that membrane complement regulatory proteins play an important role in the pathophysiology of coronary artery disease (CAD). In this study, a total of 100 individuals, with stable CAD and 100 healthy controls, both groups predominantly male, were rec...

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Autores principales: Mishra, Nitesh, Mohata, Madhav, Narang, Rajeev, Lakshmy, R., Hazarika, Anjali, Pandey, R. M., Das, Nibhriti, Luthra, Kalpana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727527/
https://www.ncbi.nlm.nih.gov/pubmed/31555286
http://dx.doi.org/10.3389/fimmu.2019.02072
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author Mishra, Nitesh
Mohata, Madhav
Narang, Rajeev
Lakshmy, R.
Hazarika, Anjali
Pandey, R. M.
Das, Nibhriti
Luthra, Kalpana
author_facet Mishra, Nitesh
Mohata, Madhav
Narang, Rajeev
Lakshmy, R.
Hazarika, Anjali
Pandey, R. M.
Das, Nibhriti
Luthra, Kalpana
author_sort Mishra, Nitesh
collection PubMed
description Studies conducted in animal models have suggested that membrane complement regulatory proteins play an important role in the pathophysiology of coronary artery disease (CAD). In this study, a total of 100 individuals, with stable CAD and 100 healthy controls, both groups predominantly male, were recruited. We evaluated the plasma levels of complement regulatory proteins (Cregs) CD35, CD46, CD55, and CD59 and their surface expression on granulocytes, lymphocytes, and monocytes by flow cytometry. The mRNA expression of these Cregs in total leukocytes was determined by quantitative PCR. The soluble forms of Cregs, C3c, Mannose binding protein-associated serine protease 2 (MASP-2), Platelet activating factor-acetyl hydrolase (PAF-AH), and inflammatory cytokines were quantified by ELISA. High plasma levels of C3c, indicative of complement activation, in addition to significantly low levels of Cregs, were observed in CAD patients. A significantly lower expression of CD46 and CD55 on the surface of lymphocytes, monocytes, and granulocytes and higher surface expression of CD35 and CD59 on granulocytes (p < 0.0001) was seen in CAD patients as compared to healthy donors. The high expression of CD59 on granulocytes positively correlated with the severity of disease and may serve as a potential marker of disease progression in CAD.
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spelling pubmed-67275272019-09-25 Altered Expression of Complement Regulatory Proteins CD35, CD46, CD55, and CD59 on Leukocyte Subsets in Individuals Suffering From Coronary Artery Disease Mishra, Nitesh Mohata, Madhav Narang, Rajeev Lakshmy, R. Hazarika, Anjali Pandey, R. M. Das, Nibhriti Luthra, Kalpana Front Immunol Immunology Studies conducted in animal models have suggested that membrane complement regulatory proteins play an important role in the pathophysiology of coronary artery disease (CAD). In this study, a total of 100 individuals, with stable CAD and 100 healthy controls, both groups predominantly male, were recruited. We evaluated the plasma levels of complement regulatory proteins (Cregs) CD35, CD46, CD55, and CD59 and their surface expression on granulocytes, lymphocytes, and monocytes by flow cytometry. The mRNA expression of these Cregs in total leukocytes was determined by quantitative PCR. The soluble forms of Cregs, C3c, Mannose binding protein-associated serine protease 2 (MASP-2), Platelet activating factor-acetyl hydrolase (PAF-AH), and inflammatory cytokines were quantified by ELISA. High plasma levels of C3c, indicative of complement activation, in addition to significantly low levels of Cregs, were observed in CAD patients. A significantly lower expression of CD46 and CD55 on the surface of lymphocytes, monocytes, and granulocytes and higher surface expression of CD35 and CD59 on granulocytes (p < 0.0001) was seen in CAD patients as compared to healthy donors. The high expression of CD59 on granulocytes positively correlated with the severity of disease and may serve as a potential marker of disease progression in CAD. Frontiers Media S.A. 2019-08-29 /pmc/articles/PMC6727527/ /pubmed/31555286 http://dx.doi.org/10.3389/fimmu.2019.02072 Text en Copyright © 2019 Mishra, Mohata, Narang, Lakshmy, Hazarika, Pandey, Das and Luthra. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Mishra, Nitesh
Mohata, Madhav
Narang, Rajeev
Lakshmy, R.
Hazarika, Anjali
Pandey, R. M.
Das, Nibhriti
Luthra, Kalpana
Altered Expression of Complement Regulatory Proteins CD35, CD46, CD55, and CD59 on Leukocyte Subsets in Individuals Suffering From Coronary Artery Disease
title Altered Expression of Complement Regulatory Proteins CD35, CD46, CD55, and CD59 on Leukocyte Subsets in Individuals Suffering From Coronary Artery Disease
title_full Altered Expression of Complement Regulatory Proteins CD35, CD46, CD55, and CD59 on Leukocyte Subsets in Individuals Suffering From Coronary Artery Disease
title_fullStr Altered Expression of Complement Regulatory Proteins CD35, CD46, CD55, and CD59 on Leukocyte Subsets in Individuals Suffering From Coronary Artery Disease
title_full_unstemmed Altered Expression of Complement Regulatory Proteins CD35, CD46, CD55, and CD59 on Leukocyte Subsets in Individuals Suffering From Coronary Artery Disease
title_short Altered Expression of Complement Regulatory Proteins CD35, CD46, CD55, and CD59 on Leukocyte Subsets in Individuals Suffering From Coronary Artery Disease
title_sort altered expression of complement regulatory proteins cd35, cd46, cd55, and cd59 on leukocyte subsets in individuals suffering from coronary artery disease
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727527/
https://www.ncbi.nlm.nih.gov/pubmed/31555286
http://dx.doi.org/10.3389/fimmu.2019.02072
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