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DNA Delivery Systems Based on Peptide-Mimicking Cationic Lipids—The Effect of the Co-Lipid on the Structure and DNA Binding Capacity
[Image: see text] In continuation of previous work, we present a new promising DNA carrier, OO4, a highly effective peptide-mimicking lysine-based cationic lipid. The structural characteristics of the polynucleotide carrier system OO4 mixed with the commonly used co-lipid DOPE and the saturated phos...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical Society
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727600/ https://www.ncbi.nlm.nih.gov/pubmed/30840475 http://dx.doi.org/10.1021/acs.langmuir.8b04139 |
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author | Tassler, Stephanie Dobner, Bodo Lampp, Lisa Ziółkowski, Robert Malinowska, Elżbieta Wölk, Christian Brezesinski, Gerald |
author_facet | Tassler, Stephanie Dobner, Bodo Lampp, Lisa Ziółkowski, Robert Malinowska, Elżbieta Wölk, Christian Brezesinski, Gerald |
author_sort | Tassler, Stephanie |
collection | PubMed |
description | [Image: see text] In continuation of previous work, we present a new promising DNA carrier, OO4, a highly effective peptide-mimicking lysine-based cationic lipid. The structural characteristics of the polynucleotide carrier system OO4 mixed with the commonly used co-lipid DOPE and the saturated phospholipid DPPE have been studied in two-dimensional and three-dimensional model systems to understand their influence on the physical–chemical properties. The phase behavior of pure OO4 and its mixtures with DOPE and DPPE was studied at the air–water interface using a Langmuir film balance combined with infrared reflection-absorption spectroscopy. In bulk, the self-assembling structures in the presence and absence of DNA were determined by small-angle and wide-angle X-ray scattering. The amount of adsorbed DNA to cationic lipid bilayers was measured using a quartz crystal microbalance. The choice of the co-lipid has an enormous influence on the structure and capability of binding DNA. DOPE promotes the formation of nonlamellar lipoplexes (cubic and hexagonal structures), whereas DPPE promotes the formation of lamellar lipoplexes. The correlation of the observed structures with the transfection efficiency and serum stability indicates that OO4/DOPE 1:3 lipoplexes with a DNA-containing cubic phase encapsulated in multilamellar structures seem to be most promising. |
format | Online Article Text |
id | pubmed-6727600 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American
Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-67276002019-09-06 DNA Delivery Systems Based on Peptide-Mimicking Cationic Lipids—The Effect of the Co-Lipid on the Structure and DNA Binding Capacity Tassler, Stephanie Dobner, Bodo Lampp, Lisa Ziółkowski, Robert Malinowska, Elżbieta Wölk, Christian Brezesinski, Gerald Langmuir [Image: see text] In continuation of previous work, we present a new promising DNA carrier, OO4, a highly effective peptide-mimicking lysine-based cationic lipid. The structural characteristics of the polynucleotide carrier system OO4 mixed with the commonly used co-lipid DOPE and the saturated phospholipid DPPE have been studied in two-dimensional and three-dimensional model systems to understand their influence on the physical–chemical properties. The phase behavior of pure OO4 and its mixtures with DOPE and DPPE was studied at the air–water interface using a Langmuir film balance combined with infrared reflection-absorption spectroscopy. In bulk, the self-assembling structures in the presence and absence of DNA were determined by small-angle and wide-angle X-ray scattering. The amount of adsorbed DNA to cationic lipid bilayers was measured using a quartz crystal microbalance. The choice of the co-lipid has an enormous influence on the structure and capability of binding DNA. DOPE promotes the formation of nonlamellar lipoplexes (cubic and hexagonal structures), whereas DPPE promotes the formation of lamellar lipoplexes. The correlation of the observed structures with the transfection efficiency and serum stability indicates that OO4/DOPE 1:3 lipoplexes with a DNA-containing cubic phase encapsulated in multilamellar structures seem to be most promising. American Chemical Society 2019-03-06 2019-04-02 /pmc/articles/PMC6727600/ /pubmed/30840475 http://dx.doi.org/10.1021/acs.langmuir.8b04139 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Tassler, Stephanie Dobner, Bodo Lampp, Lisa Ziółkowski, Robert Malinowska, Elżbieta Wölk, Christian Brezesinski, Gerald DNA Delivery Systems Based on Peptide-Mimicking Cationic Lipids—The Effect of the Co-Lipid on the Structure and DNA Binding Capacity |
title | DNA Delivery Systems Based on Peptide-Mimicking Cationic
Lipids—The Effect of the Co-Lipid on the Structure and DNA
Binding Capacity |
title_full | DNA Delivery Systems Based on Peptide-Mimicking Cationic
Lipids—The Effect of the Co-Lipid on the Structure and DNA
Binding Capacity |
title_fullStr | DNA Delivery Systems Based on Peptide-Mimicking Cationic
Lipids—The Effect of the Co-Lipid on the Structure and DNA
Binding Capacity |
title_full_unstemmed | DNA Delivery Systems Based on Peptide-Mimicking Cationic
Lipids—The Effect of the Co-Lipid on the Structure and DNA
Binding Capacity |
title_short | DNA Delivery Systems Based on Peptide-Mimicking Cationic
Lipids—The Effect of the Co-Lipid on the Structure and DNA
Binding Capacity |
title_sort | dna delivery systems based on peptide-mimicking cationic
lipids—the effect of the co-lipid on the structure and dna
binding capacity |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727600/ https://www.ncbi.nlm.nih.gov/pubmed/30840475 http://dx.doi.org/10.1021/acs.langmuir.8b04139 |
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