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Shape and Phase Transitions in a PEGylated Phospholipid System
[Image: see text] Poly(ethylene glycol) (PEG) polymers and PEG-conjugated lipids are widely used in bioengineering and drug transport applications. A PEG layer in a drug carrier increases hydrophilic repulsion, inhibits membrane fusion and serum opsonin interactions, and prolongs the storage and cir...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical Society
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727609/ https://www.ncbi.nlm.nih.gov/pubmed/30789270 http://dx.doi.org/10.1021/acs.langmuir.8b03829 |
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author | Viitala, Lauri Pajari, Saija Gentile, Luigi Määttä, Jukka Gubitosi, Marta Deska, Jan Sammalkorpi, Maria Olsson, Ulf Murtomäki, Lasse |
author_facet | Viitala, Lauri Pajari, Saija Gentile, Luigi Määttä, Jukka Gubitosi, Marta Deska, Jan Sammalkorpi, Maria Olsson, Ulf Murtomäki, Lasse |
author_sort | Viitala, Lauri |
collection | PubMed |
description | [Image: see text] Poly(ethylene glycol) (PEG) polymers and PEG-conjugated lipids are widely used in bioengineering and drug transport applications. A PEG layer in a drug carrier increases hydrophilic repulsion, inhibits membrane fusion and serum opsonin interactions, and prolongs the storage and circulation time. It can also change the carrier shape and have an influence on many properties related to the content release of the carrier. In this paper, we focus on the physicochemical effects of PEGylation in the lipid bilayer. We introduce laurdanC as a fluorophore for shape recognition and phase transition detection. Together with laurdanC, cryogenic transmission electron microscopy, differential scanning calorimetry, molecular dynamics simulations, and small-angle X-ray scattering/wide-angle X-ray scattering, we acquire information of the particle/bilayer morphology and phase behavior in systems containing 1,2-dipalmitoyl-sn-glycero-3-phosphocholine:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-PEG(2000) with different fractions. We find that PEGylation leads to two important and potentially usable features of the system. (1) Spherical vesicles present a window of elevated chain-melting temperatures and (2) lipid packing shape-controlled liposome-to-bicelle transition. The first finding is significant for targets requiring multiple release sequences and the second enables tuning the release by composition and the PEG polymer length. Besides drug delivery systems, the findings can be used in other smart soft materials with trigger-polymers as well. |
format | Online Article Text |
id | pubmed-6727609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American
Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-67276092019-09-06 Shape and Phase Transitions in a PEGylated Phospholipid System Viitala, Lauri Pajari, Saija Gentile, Luigi Määttä, Jukka Gubitosi, Marta Deska, Jan Sammalkorpi, Maria Olsson, Ulf Murtomäki, Lasse Langmuir [Image: see text] Poly(ethylene glycol) (PEG) polymers and PEG-conjugated lipids are widely used in bioengineering and drug transport applications. A PEG layer in a drug carrier increases hydrophilic repulsion, inhibits membrane fusion and serum opsonin interactions, and prolongs the storage and circulation time. It can also change the carrier shape and have an influence on many properties related to the content release of the carrier. In this paper, we focus on the physicochemical effects of PEGylation in the lipid bilayer. We introduce laurdanC as a fluorophore for shape recognition and phase transition detection. Together with laurdanC, cryogenic transmission electron microscopy, differential scanning calorimetry, molecular dynamics simulations, and small-angle X-ray scattering/wide-angle X-ray scattering, we acquire information of the particle/bilayer morphology and phase behavior in systems containing 1,2-dipalmitoyl-sn-glycero-3-phosphocholine:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-PEG(2000) with different fractions. We find that PEGylation leads to two important and potentially usable features of the system. (1) Spherical vesicles present a window of elevated chain-melting temperatures and (2) lipid packing shape-controlled liposome-to-bicelle transition. The first finding is significant for targets requiring multiple release sequences and the second enables tuning the release by composition and the PEG polymer length. Besides drug delivery systems, the findings can be used in other smart soft materials with trigger-polymers as well. American Chemical Society 2019-02-21 2019-03-19 /pmc/articles/PMC6727609/ /pubmed/30789270 http://dx.doi.org/10.1021/acs.langmuir.8b03829 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Viitala, Lauri Pajari, Saija Gentile, Luigi Määttä, Jukka Gubitosi, Marta Deska, Jan Sammalkorpi, Maria Olsson, Ulf Murtomäki, Lasse Shape and Phase Transitions in a PEGylated Phospholipid System |
title | Shape and
Phase Transitions in a PEGylated Phospholipid System |
title_full | Shape and
Phase Transitions in a PEGylated Phospholipid System |
title_fullStr | Shape and
Phase Transitions in a PEGylated Phospholipid System |
title_full_unstemmed | Shape and
Phase Transitions in a PEGylated Phospholipid System |
title_short | Shape and
Phase Transitions in a PEGylated Phospholipid System |
title_sort | shape and
phase transitions in a pegylated phospholipid system |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727609/ https://www.ncbi.nlm.nih.gov/pubmed/30789270 http://dx.doi.org/10.1021/acs.langmuir.8b03829 |
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