Cargando…

Force balances between interphase centrosomes as revealed by laser ablation

Numerous studies have highlighted the self-centering activities of individual microtubule (MT) arrays in animal cells, but relatively few works address the behavior of multiple arrays that coexist in a common cytoplasm. In multinucleated Dictyostelium discoideum cells, each centrosome organizes a ra...

Descripción completa

Detalles Bibliográficos
Autores principales: Odell, Jacob, Sikirzhytski, Vitali, Tikhonenko, Irina, Cobani, Sonila, Khodjakov, Alexey, Koonce, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727758/
https://www.ncbi.nlm.nih.gov/pubmed/31067156
http://dx.doi.org/10.1091/mbc.E19-01-0034
_version_ 1783449318824345600
author Odell, Jacob
Sikirzhytski, Vitali
Tikhonenko, Irina
Cobani, Sonila
Khodjakov, Alexey
Koonce, Michael
author_facet Odell, Jacob
Sikirzhytski, Vitali
Tikhonenko, Irina
Cobani, Sonila
Khodjakov, Alexey
Koonce, Michael
author_sort Odell, Jacob
collection PubMed
description Numerous studies have highlighted the self-centering activities of individual microtubule (MT) arrays in animal cells, but relatively few works address the behavior of multiple arrays that coexist in a common cytoplasm. In multinucleated Dictyostelium discoideum cells, each centrosome organizes a radial MT network, and these networks remain separate from one another. This feature offers an opportunity to reveal the mechanism(s) responsible for the positioning of multiple centrosomes. Using a laser microbeam to eliminate one of the two centrosomes in binucleate cells, we show that the unaltered array is rapidly repositioned at the cell center. This result demonstrates that each MT array is constantly subject to centering forces and infers a mechanism to balance the positions of multiple arrays. Our results address the limited actions of three kinesins and a cross-linking MAP that are known to have effects in maintaining MT organization and suggest a simple means used to keep the arrays separated.
format Online
Article
Text
id pubmed-6727758
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-67277582019-09-16 Force balances between interphase centrosomes as revealed by laser ablation Odell, Jacob Sikirzhytski, Vitali Tikhonenko, Irina Cobani, Sonila Khodjakov, Alexey Koonce, Michael Mol Biol Cell Articles Numerous studies have highlighted the self-centering activities of individual microtubule (MT) arrays in animal cells, but relatively few works address the behavior of multiple arrays that coexist in a common cytoplasm. In multinucleated Dictyostelium discoideum cells, each centrosome organizes a radial MT network, and these networks remain separate from one another. This feature offers an opportunity to reveal the mechanism(s) responsible for the positioning of multiple centrosomes. Using a laser microbeam to eliminate one of the two centrosomes in binucleate cells, we show that the unaltered array is rapidly repositioned at the cell center. This result demonstrates that each MT array is constantly subject to centering forces and infers a mechanism to balance the positions of multiple arrays. Our results address the limited actions of three kinesins and a cross-linking MAP that are known to have effects in maintaining MT organization and suggest a simple means used to keep the arrays separated. The American Society for Cell Biology 2019-07-01 /pmc/articles/PMC6727758/ /pubmed/31067156 http://dx.doi.org/10.1091/mbc.E19-01-0034 Text en © 2019 Odell et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Odell, Jacob
Sikirzhytski, Vitali
Tikhonenko, Irina
Cobani, Sonila
Khodjakov, Alexey
Koonce, Michael
Force balances between interphase centrosomes as revealed by laser ablation
title Force balances between interphase centrosomes as revealed by laser ablation
title_full Force balances between interphase centrosomes as revealed by laser ablation
title_fullStr Force balances between interphase centrosomes as revealed by laser ablation
title_full_unstemmed Force balances between interphase centrosomes as revealed by laser ablation
title_short Force balances between interphase centrosomes as revealed by laser ablation
title_sort force balances between interphase centrosomes as revealed by laser ablation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727758/
https://www.ncbi.nlm.nih.gov/pubmed/31067156
http://dx.doi.org/10.1091/mbc.E19-01-0034
work_keys_str_mv AT odelljacob forcebalancesbetweeninterphasecentrosomesasrevealedbylaserablation
AT sikirzhytskivitali forcebalancesbetweeninterphasecentrosomesasrevealedbylaserablation
AT tikhonenkoirina forcebalancesbetweeninterphasecentrosomesasrevealedbylaserablation
AT cobanisonila forcebalancesbetweeninterphasecentrosomesasrevealedbylaserablation
AT khodjakovalexey forcebalancesbetweeninterphasecentrosomesasrevealedbylaserablation
AT kooncemichael forcebalancesbetweeninterphasecentrosomesasrevealedbylaserablation