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Force balances between interphase centrosomes as revealed by laser ablation
Numerous studies have highlighted the self-centering activities of individual microtubule (MT) arrays in animal cells, but relatively few works address the behavior of multiple arrays that coexist in a common cytoplasm. In multinucleated Dictyostelium discoideum cells, each centrosome organizes a ra...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727758/ https://www.ncbi.nlm.nih.gov/pubmed/31067156 http://dx.doi.org/10.1091/mbc.E19-01-0034 |
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author | Odell, Jacob Sikirzhytski, Vitali Tikhonenko, Irina Cobani, Sonila Khodjakov, Alexey Koonce, Michael |
author_facet | Odell, Jacob Sikirzhytski, Vitali Tikhonenko, Irina Cobani, Sonila Khodjakov, Alexey Koonce, Michael |
author_sort | Odell, Jacob |
collection | PubMed |
description | Numerous studies have highlighted the self-centering activities of individual microtubule (MT) arrays in animal cells, but relatively few works address the behavior of multiple arrays that coexist in a common cytoplasm. In multinucleated Dictyostelium discoideum cells, each centrosome organizes a radial MT network, and these networks remain separate from one another. This feature offers an opportunity to reveal the mechanism(s) responsible for the positioning of multiple centrosomes. Using a laser microbeam to eliminate one of the two centrosomes in binucleate cells, we show that the unaltered array is rapidly repositioned at the cell center. This result demonstrates that each MT array is constantly subject to centering forces and infers a mechanism to balance the positions of multiple arrays. Our results address the limited actions of three kinesins and a cross-linking MAP that are known to have effects in maintaining MT organization and suggest a simple means used to keep the arrays separated. |
format | Online Article Text |
id | pubmed-6727758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-67277582019-09-16 Force balances between interphase centrosomes as revealed by laser ablation Odell, Jacob Sikirzhytski, Vitali Tikhonenko, Irina Cobani, Sonila Khodjakov, Alexey Koonce, Michael Mol Biol Cell Articles Numerous studies have highlighted the self-centering activities of individual microtubule (MT) arrays in animal cells, but relatively few works address the behavior of multiple arrays that coexist in a common cytoplasm. In multinucleated Dictyostelium discoideum cells, each centrosome organizes a radial MT network, and these networks remain separate from one another. This feature offers an opportunity to reveal the mechanism(s) responsible for the positioning of multiple centrosomes. Using a laser microbeam to eliminate one of the two centrosomes in binucleate cells, we show that the unaltered array is rapidly repositioned at the cell center. This result demonstrates that each MT array is constantly subject to centering forces and infers a mechanism to balance the positions of multiple arrays. Our results address the limited actions of three kinesins and a cross-linking MAP that are known to have effects in maintaining MT organization and suggest a simple means used to keep the arrays separated. The American Society for Cell Biology 2019-07-01 /pmc/articles/PMC6727758/ /pubmed/31067156 http://dx.doi.org/10.1091/mbc.E19-01-0034 Text en © 2019 Odell et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License. |
spellingShingle | Articles Odell, Jacob Sikirzhytski, Vitali Tikhonenko, Irina Cobani, Sonila Khodjakov, Alexey Koonce, Michael Force balances between interphase centrosomes as revealed by laser ablation |
title | Force balances between interphase centrosomes as revealed by laser ablation |
title_full | Force balances between interphase centrosomes as revealed by laser ablation |
title_fullStr | Force balances between interphase centrosomes as revealed by laser ablation |
title_full_unstemmed | Force balances between interphase centrosomes as revealed by laser ablation |
title_short | Force balances between interphase centrosomes as revealed by laser ablation |
title_sort | force balances between interphase centrosomes as revealed by laser ablation |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727758/ https://www.ncbi.nlm.nih.gov/pubmed/31067156 http://dx.doi.org/10.1091/mbc.E19-01-0034 |
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