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lnc-SAMD14-4 can regulate expression of the COL1A1 and COL1A2 in human chondrocytes

Osteoarthritis (OA) is the most common motor system disease in aging people, characterized by matrix degradation, chondrocyte death, and osteophyte formation. OA etiology is unclear, but long noncoding RNAs (lncRNAs) that participate in numerous pathological and physiological processes may be key re...

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Autores principales: Zhang, Haibin, Chen, Cheng, Cui, Yinghong, Li, Yuqing, Wang, Zhaojun, Mao, Xinzhan, Dou, Pengcheng, Li, Yihan, Ma, Chi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727836/
https://www.ncbi.nlm.nih.gov/pubmed/31534838
http://dx.doi.org/10.7717/peerj.7491
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author Zhang, Haibin
Chen, Cheng
Cui, Yinghong
Li, Yuqing
Wang, Zhaojun
Mao, Xinzhan
Dou, Pengcheng
Li, Yihan
Ma, Chi
author_facet Zhang, Haibin
Chen, Cheng
Cui, Yinghong
Li, Yuqing
Wang, Zhaojun
Mao, Xinzhan
Dou, Pengcheng
Li, Yihan
Ma, Chi
author_sort Zhang, Haibin
collection PubMed
description Osteoarthritis (OA) is the most common motor system disease in aging people, characterized by matrix degradation, chondrocyte death, and osteophyte formation. OA etiology is unclear, but long noncoding RNAs (lncRNAs) that participate in numerous pathological and physiological processes may be key regulators in the onset and development of OA. Because profiling of lncRNAs and their biological function in OA is not understood, we measured lncRNA and mRNA expression profiles using high-throughput microarray to study human knee OA. We identified 2,042 lncRNAs and 2,011 mRNAs that were significantly differentially expressed in OA compared to non-OA tissue (>2.0- or < − 2.0-fold change; p < 0.5), including 1,137 lncRNAs that were upregulated and 905 lncRNAs that were downregulated. Also, 1,386 mRNA were upregulated and 625 mRNAs were downregulated. QPCR was used to validate chip results. Gene Ontology analysis and the Kyoto Encyclopedia of Genes and Genomes was used to study the biological function enrichment of differentially expressed mRNA. Additionally, coding-non-coding gene co-expression (CNC) network construction was performed to explore the relevance of dysregulated lncRNAs and mRNAs. Finally, the gain/loss of function experiments of lnc-SAMD14-4 was implemented in IL-1β-treated human chondrocytes. In general, this study provides a preliminary database for further exploring lncRNA-related mechnisms in OA.
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spelling pubmed-67278362019-09-18 lnc-SAMD14-4 can regulate expression of the COL1A1 and COL1A2 in human chondrocytes Zhang, Haibin Chen, Cheng Cui, Yinghong Li, Yuqing Wang, Zhaojun Mao, Xinzhan Dou, Pengcheng Li, Yihan Ma, Chi PeerJ Genetics Osteoarthritis (OA) is the most common motor system disease in aging people, characterized by matrix degradation, chondrocyte death, and osteophyte formation. OA etiology is unclear, but long noncoding RNAs (lncRNAs) that participate in numerous pathological and physiological processes may be key regulators in the onset and development of OA. Because profiling of lncRNAs and their biological function in OA is not understood, we measured lncRNA and mRNA expression profiles using high-throughput microarray to study human knee OA. We identified 2,042 lncRNAs and 2,011 mRNAs that were significantly differentially expressed in OA compared to non-OA tissue (>2.0- or < − 2.0-fold change; p < 0.5), including 1,137 lncRNAs that were upregulated and 905 lncRNAs that were downregulated. Also, 1,386 mRNA were upregulated and 625 mRNAs were downregulated. QPCR was used to validate chip results. Gene Ontology analysis and the Kyoto Encyclopedia of Genes and Genomes was used to study the biological function enrichment of differentially expressed mRNA. Additionally, coding-non-coding gene co-expression (CNC) network construction was performed to explore the relevance of dysregulated lncRNAs and mRNAs. Finally, the gain/loss of function experiments of lnc-SAMD14-4 was implemented in IL-1β-treated human chondrocytes. In general, this study provides a preliminary database for further exploring lncRNA-related mechnisms in OA. PeerJ Inc. 2019-09-02 /pmc/articles/PMC6727836/ /pubmed/31534838 http://dx.doi.org/10.7717/peerj.7491 Text en ©2019 Zhang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Genetics
Zhang, Haibin
Chen, Cheng
Cui, Yinghong
Li, Yuqing
Wang, Zhaojun
Mao, Xinzhan
Dou, Pengcheng
Li, Yihan
Ma, Chi
lnc-SAMD14-4 can regulate expression of the COL1A1 and COL1A2 in human chondrocytes
title lnc-SAMD14-4 can regulate expression of the COL1A1 and COL1A2 in human chondrocytes
title_full lnc-SAMD14-4 can regulate expression of the COL1A1 and COL1A2 in human chondrocytes
title_fullStr lnc-SAMD14-4 can regulate expression of the COL1A1 and COL1A2 in human chondrocytes
title_full_unstemmed lnc-SAMD14-4 can regulate expression of the COL1A1 and COL1A2 in human chondrocytes
title_short lnc-SAMD14-4 can regulate expression of the COL1A1 and COL1A2 in human chondrocytes
title_sort lnc-samd14-4 can regulate expression of the col1a1 and col1a2 in human chondrocytes
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6727836/
https://www.ncbi.nlm.nih.gov/pubmed/31534838
http://dx.doi.org/10.7717/peerj.7491
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