High-throughput determination of the antigen specificities of T cell receptors in single cells

We present tetramer-associated T-cell receptor sequencing (TetTCR-Seq), a method to link T cell receptor (TCR) sequences to their cognate antigens in single cells at high throughput. Binding is determined using a library of DNA-barcoded antigen tetramers that is rapidly generated by in vitro transcr...

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Detalles Bibliográficos
Autores principales: Zhang, Shu-Qi, Ma, Ke-Yue, Schonnesen, Alexandra A., Zhang, Mingliang, He, Chenfeng, Sun, Eric, Williams, Chad M., Jia, Weiping, Jiang, Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6728224/
https://www.ncbi.nlm.nih.gov/pubmed/30418433
http://dx.doi.org/10.1038/nbt.4282
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author Zhang, Shu-Qi
Ma, Ke-Yue
Schonnesen, Alexandra A.
Zhang, Mingliang
He, Chenfeng
Sun, Eric
Williams, Chad M.
Jia, Weiping
Jiang, Ning
author_facet Zhang, Shu-Qi
Ma, Ke-Yue
Schonnesen, Alexandra A.
Zhang, Mingliang
He, Chenfeng
Sun, Eric
Williams, Chad M.
Jia, Weiping
Jiang, Ning
author_sort Zhang, Shu-Qi
collection PubMed
description We present tetramer-associated T-cell receptor sequencing (TetTCR-Seq), a method to link T cell receptor (TCR) sequences to their cognate antigens in single cells at high throughput. Binding is determined using a library of DNA-barcoded antigen tetramers that is rapidly generated by in vitro transcription and translation. We applied TetTCR-Seq to identify patterns in TCR cross-reactivity with cancer neo-antigens and to rapidly isolate neo-antigen-specific TCRs with no cross-reactivity to the wild-type antigen.
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spelling pubmed-67282242019-09-06 High-throughput determination of the antigen specificities of T cell receptors in single cells Zhang, Shu-Qi Ma, Ke-Yue Schonnesen, Alexandra A. Zhang, Mingliang He, Chenfeng Sun, Eric Williams, Chad M. Jia, Weiping Jiang, Ning Nat Biotechnol Article We present tetramer-associated T-cell receptor sequencing (TetTCR-Seq), a method to link T cell receptor (TCR) sequences to their cognate antigens in single cells at high throughput. Binding is determined using a library of DNA-barcoded antigen tetramers that is rapidly generated by in vitro transcription and translation. We applied TetTCR-Seq to identify patterns in TCR cross-reactivity with cancer neo-antigens and to rapidly isolate neo-antigen-specific TCRs with no cross-reactivity to the wild-type antigen. 2018-11-12 /pmc/articles/PMC6728224/ /pubmed/30418433 http://dx.doi.org/10.1038/nbt.4282 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Zhang, Shu-Qi
Ma, Ke-Yue
Schonnesen, Alexandra A.
Zhang, Mingliang
He, Chenfeng
Sun, Eric
Williams, Chad M.
Jia, Weiping
Jiang, Ning
High-throughput determination of the antigen specificities of T cell receptors in single cells
title High-throughput determination of the antigen specificities of T cell receptors in single cells
title_full High-throughput determination of the antigen specificities of T cell receptors in single cells
title_fullStr High-throughput determination of the antigen specificities of T cell receptors in single cells
title_full_unstemmed High-throughput determination of the antigen specificities of T cell receptors in single cells
title_short High-throughput determination of the antigen specificities of T cell receptors in single cells
title_sort high-throughput determination of the antigen specificities of t cell receptors in single cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6728224/
https://www.ncbi.nlm.nih.gov/pubmed/30418433
http://dx.doi.org/10.1038/nbt.4282
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