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Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla
The aquatic weed Azolla is a potential protein crop due to its prolific growth and high protein content, supported entirely by nitrogen-fixing symbionts. Alkaline protein extraction at pH 8 followed by acid precipitation allowed recovery of 16–26% of the biomass nitrogen, while at pH 10.5 nitrogen r...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6728274/ https://www.ncbi.nlm.nih.gov/pubmed/31516849 http://dx.doi.org/10.1016/j.btre.2019.e00368 |
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author | Brouwer, Paul Nierop, Klaas G.J. Huijgen, Wouter J.J. Schluepmann, Henriette |
author_facet | Brouwer, Paul Nierop, Klaas G.J. Huijgen, Wouter J.J. Schluepmann, Henriette |
author_sort | Brouwer, Paul |
collection | PubMed |
description | The aquatic weed Azolla is a potential protein crop due to its prolific growth and high protein content, supported entirely by nitrogen-fixing symbionts. Alkaline protein extraction at pH 8 followed by acid precipitation allowed recovery of 16–26% of the biomass nitrogen, while at pH 10.5 nitrogen recovery improved to 35–54%. This pH effect was typical of ferns of the family Salviniaceae, and may be explained by high concentrations of condensed tannins (CTs) in the biomass that precipitate protein at mild pH. Two approaches were tested to increase protein yield and reduce protein binding by CTs. Pre-extraction with aqueous acetone (70 v/v%) removed 76–85% of the CTs and subsequent alkaline extraction at pH 12.5 and 95 °C recovered 38% of the biomass nitrogen. Extraction with 1.5% of PEG as a CT-binding agent, also permitted to recover 38% of the nitrogen, under milder conditions of pH 8 and 45 °C. |
format | Online Article Text |
id | pubmed-6728274 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-67282742019-09-12 Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla Brouwer, Paul Nierop, Klaas G.J. Huijgen, Wouter J.J. Schluepmann, Henriette Biotechnol Rep (Amst) Research Article The aquatic weed Azolla is a potential protein crop due to its prolific growth and high protein content, supported entirely by nitrogen-fixing symbionts. Alkaline protein extraction at pH 8 followed by acid precipitation allowed recovery of 16–26% of the biomass nitrogen, while at pH 10.5 nitrogen recovery improved to 35–54%. This pH effect was typical of ferns of the family Salviniaceae, and may be explained by high concentrations of condensed tannins (CTs) in the biomass that precipitate protein at mild pH. Two approaches were tested to increase protein yield and reduce protein binding by CTs. Pre-extraction with aqueous acetone (70 v/v%) removed 76–85% of the CTs and subsequent alkaline extraction at pH 12.5 and 95 °C recovered 38% of the biomass nitrogen. Extraction with 1.5% of PEG as a CT-binding agent, also permitted to recover 38% of the nitrogen, under milder conditions of pH 8 and 45 °C. Elsevier 2019-08-11 /pmc/articles/PMC6728274/ /pubmed/31516849 http://dx.doi.org/10.1016/j.btre.2019.e00368 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Brouwer, Paul Nierop, Klaas G.J. Huijgen, Wouter J.J. Schluepmann, Henriette Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla |
title | Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla |
title_full | Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla |
title_fullStr | Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla |
title_full_unstemmed | Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla |
title_short | Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla |
title_sort | aquatic weeds as novel protein sources: alkaline extraction of tannin-rich azolla |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6728274/ https://www.ncbi.nlm.nih.gov/pubmed/31516849 http://dx.doi.org/10.1016/j.btre.2019.e00368 |
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