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Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla

The aquatic weed Azolla is a potential protein crop due to its prolific growth and high protein content, supported entirely by nitrogen-fixing symbionts. Alkaline protein extraction at pH 8 followed by acid precipitation allowed recovery of 16–26% of the biomass nitrogen, while at pH 10.5 nitrogen r...

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Detalles Bibliográficos
Autores principales: Brouwer, Paul, Nierop, Klaas G.J., Huijgen, Wouter J.J., Schluepmann, Henriette
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6728274/
https://www.ncbi.nlm.nih.gov/pubmed/31516849
http://dx.doi.org/10.1016/j.btre.2019.e00368
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author Brouwer, Paul
Nierop, Klaas G.J.
Huijgen, Wouter J.J.
Schluepmann, Henriette
author_facet Brouwer, Paul
Nierop, Klaas G.J.
Huijgen, Wouter J.J.
Schluepmann, Henriette
author_sort Brouwer, Paul
collection PubMed
description The aquatic weed Azolla is a potential protein crop due to its prolific growth and high protein content, supported entirely by nitrogen-fixing symbionts. Alkaline protein extraction at pH 8 followed by acid precipitation allowed recovery of 16–26% of the biomass nitrogen, while at pH 10.5 nitrogen recovery improved to 35–54%. This pH effect was typical of ferns of the family Salviniaceae, and may be explained by high concentrations of condensed tannins (CTs) in the biomass that precipitate protein at mild pH. Two approaches were tested to increase protein yield and reduce protein binding by CTs. Pre-extraction with aqueous acetone (70 v/v%) removed 76–85% of the CTs and subsequent alkaline extraction at pH 12.5 and 95 °C recovered 38% of the biomass nitrogen. Extraction with 1.5% of PEG as a CT-binding agent, also permitted to recover 38% of the nitrogen, under milder conditions of pH 8 and 45 °C.
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spelling pubmed-67282742019-09-12 Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla Brouwer, Paul Nierop, Klaas G.J. Huijgen, Wouter J.J. Schluepmann, Henriette Biotechnol Rep (Amst) Research Article The aquatic weed Azolla is a potential protein crop due to its prolific growth and high protein content, supported entirely by nitrogen-fixing symbionts. Alkaline protein extraction at pH 8 followed by acid precipitation allowed recovery of 16–26% of the biomass nitrogen, while at pH 10.5 nitrogen recovery improved to 35–54%. This pH effect was typical of ferns of the family Salviniaceae, and may be explained by high concentrations of condensed tannins (CTs) in the biomass that precipitate protein at mild pH. Two approaches were tested to increase protein yield and reduce protein binding by CTs. Pre-extraction with aqueous acetone (70 v/v%) removed 76–85% of the CTs and subsequent alkaline extraction at pH 12.5 and 95 °C recovered 38% of the biomass nitrogen. Extraction with 1.5% of PEG as a CT-binding agent, also permitted to recover 38% of the nitrogen, under milder conditions of pH 8 and 45 °C. Elsevier 2019-08-11 /pmc/articles/PMC6728274/ /pubmed/31516849 http://dx.doi.org/10.1016/j.btre.2019.e00368 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Brouwer, Paul
Nierop, Klaas G.J.
Huijgen, Wouter J.J.
Schluepmann, Henriette
Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla
title Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla
title_full Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla
title_fullStr Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla
title_full_unstemmed Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla
title_short Aquatic weeds as novel protein sources: Alkaline extraction of tannin-rich Azolla
title_sort aquatic weeds as novel protein sources: alkaline extraction of tannin-rich azolla
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6728274/
https://www.ncbi.nlm.nih.gov/pubmed/31516849
http://dx.doi.org/10.1016/j.btre.2019.e00368
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