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A High-Resolution Map of SBP1 Interactomes in Plasmodium falciparum-infected Erythrocytes
The pathogenesis of malaria parasites depends on host erythrocyte modifications that are facilitated by parasite proteins exported to the host cytoplasm. These exported proteins form a trafficking complex in the host cytoplasm that transports virulence determinants to the erythrocyte surface; this c...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6728614/ https://www.ncbi.nlm.nih.gov/pubmed/31476617 http://dx.doi.org/10.1016/j.isci.2019.07.035 |
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author | Takano, Ryo Kozuka-Hata, Hiroko Kondoh, Daisuke Bochimoto, Hiroki Oyama, Masaaki Kato, Kentaro |
author_facet | Takano, Ryo Kozuka-Hata, Hiroko Kondoh, Daisuke Bochimoto, Hiroki Oyama, Masaaki Kato, Kentaro |
author_sort | Takano, Ryo |
collection | PubMed |
description | The pathogenesis of malaria parasites depends on host erythrocyte modifications that are facilitated by parasite proteins exported to the host cytoplasm. These exported proteins form a trafficking complex in the host cytoplasm that transports virulence determinants to the erythrocyte surface; this complex is thus essential for malaria virulence. Here, we report a comprehensive interaction network map of this complex. We developed authentic, unbiased, highly sensitive proteomic approaches to determine the proteins that interact with a core component of the complex, SBP1 (skeleton-binding protein 1). SBP1 interactomes revealed numerous exported proteins and potential interactors associated with SBP1 intracellular trafficking. We identified several host-parasite protein interactions and linked the exported protein MAL8P1.4 to Plasmodium falciparum virulence in infected erythrocytes. Our study highlights the complicated interplay between parasite and host proteins in the host cytoplasm and provides an interaction dataset connecting dozens of exported proteins required for P. falciparum virulence. |
format | Online Article Text |
id | pubmed-6728614 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-67286142019-09-12 A High-Resolution Map of SBP1 Interactomes in Plasmodium falciparum-infected Erythrocytes Takano, Ryo Kozuka-Hata, Hiroko Kondoh, Daisuke Bochimoto, Hiroki Oyama, Masaaki Kato, Kentaro iScience Article The pathogenesis of malaria parasites depends on host erythrocyte modifications that are facilitated by parasite proteins exported to the host cytoplasm. These exported proteins form a trafficking complex in the host cytoplasm that transports virulence determinants to the erythrocyte surface; this complex is thus essential for malaria virulence. Here, we report a comprehensive interaction network map of this complex. We developed authentic, unbiased, highly sensitive proteomic approaches to determine the proteins that interact with a core component of the complex, SBP1 (skeleton-binding protein 1). SBP1 interactomes revealed numerous exported proteins and potential interactors associated with SBP1 intracellular trafficking. We identified several host-parasite protein interactions and linked the exported protein MAL8P1.4 to Plasmodium falciparum virulence in infected erythrocytes. Our study highlights the complicated interplay between parasite and host proteins in the host cytoplasm and provides an interaction dataset connecting dozens of exported proteins required for P. falciparum virulence. Elsevier 2019-07-25 /pmc/articles/PMC6728614/ /pubmed/31476617 http://dx.doi.org/10.1016/j.isci.2019.07.035 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Takano, Ryo Kozuka-Hata, Hiroko Kondoh, Daisuke Bochimoto, Hiroki Oyama, Masaaki Kato, Kentaro A High-Resolution Map of SBP1 Interactomes in Plasmodium falciparum-infected Erythrocytes |
title | A High-Resolution Map of SBP1 Interactomes in Plasmodium falciparum-infected Erythrocytes |
title_full | A High-Resolution Map of SBP1 Interactomes in Plasmodium falciparum-infected Erythrocytes |
title_fullStr | A High-Resolution Map of SBP1 Interactomes in Plasmodium falciparum-infected Erythrocytes |
title_full_unstemmed | A High-Resolution Map of SBP1 Interactomes in Plasmodium falciparum-infected Erythrocytes |
title_short | A High-Resolution Map of SBP1 Interactomes in Plasmodium falciparum-infected Erythrocytes |
title_sort | high-resolution map of sbp1 interactomes in plasmodium falciparum-infected erythrocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6728614/ https://www.ncbi.nlm.nih.gov/pubmed/31476617 http://dx.doi.org/10.1016/j.isci.2019.07.035 |
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