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Capability for arsenic mobilization in groundwater is distributed across broad phylogenetic lineages

Despite the importance of microbial activity in mobilizing arsenic in groundwater aquifers, the phylogenetic distribution of contributing microbial metabolisms is understudied. Groundwater samples from Ohio aquifers were analyzed using metagenomic sequencing to identify functional potential that cou...

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Autores principales: Danczak, Robert E., Johnston, Michael D., Kenah, Chris, Slattery, Michael, Wilkins, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6730927/
https://www.ncbi.nlm.nih.gov/pubmed/31490939
http://dx.doi.org/10.1371/journal.pone.0221694
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author Danczak, Robert E.
Johnston, Michael D.
Kenah, Chris
Slattery, Michael
Wilkins, Michael J.
author_facet Danczak, Robert E.
Johnston, Michael D.
Kenah, Chris
Slattery, Michael
Wilkins, Michael J.
author_sort Danczak, Robert E.
collection PubMed
description Despite the importance of microbial activity in mobilizing arsenic in groundwater aquifers, the phylogenetic distribution of contributing microbial metabolisms is understudied. Groundwater samples from Ohio aquifers were analyzed using metagenomic sequencing to identify functional potential that could drive arsenic cycling, and revealed mechanisms for direct (i.e., Ars system) and indirect (i.e., iron reduction) arsenic mobilization in all samples, despite differing geochemical conditions. Analyses of 194 metagenome-assembled genomes (MAGs) revealed widespread functionality related to arsenic mobilization throughout the bacterial tree of life. While arsB and arsC genes (components of an arsenic resistance system) were found in diverse lineages with no apparent phylogenetic bias, putative aioA genes (aerobic arsenite oxidase) were predominantly identified in Methylocystaceae MAGs. Both previously described and undescribed respiratory arsenate reduction potential via arrA was detected in Betaproteobacteria, Deltaproteobacteria, and Nitrospirae MAGs, whereas sulfate reduction potential was primarily limited to members of the Deltaproteobacteria and Nitrospirae. Lastly, iron reduction potential was detected in the Ignavibacteria, Deltaproteobacteria, and Nitrospirae. These results expand the phylogenetic distribution of taxa that may play roles in arsenic mobilization in subsurface systems. Specifically, the Nitrospirae are a much more functionally diverse group than previously assumed and may play key biogeochemical roles in arsenic-contaminated ecosystems.
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spelling pubmed-67309272019-09-16 Capability for arsenic mobilization in groundwater is distributed across broad phylogenetic lineages Danczak, Robert E. Johnston, Michael D. Kenah, Chris Slattery, Michael Wilkins, Michael J. PLoS One Research Article Despite the importance of microbial activity in mobilizing arsenic in groundwater aquifers, the phylogenetic distribution of contributing microbial metabolisms is understudied. Groundwater samples from Ohio aquifers were analyzed using metagenomic sequencing to identify functional potential that could drive arsenic cycling, and revealed mechanisms for direct (i.e., Ars system) and indirect (i.e., iron reduction) arsenic mobilization in all samples, despite differing geochemical conditions. Analyses of 194 metagenome-assembled genomes (MAGs) revealed widespread functionality related to arsenic mobilization throughout the bacterial tree of life. While arsB and arsC genes (components of an arsenic resistance system) were found in diverse lineages with no apparent phylogenetic bias, putative aioA genes (aerobic arsenite oxidase) were predominantly identified in Methylocystaceae MAGs. Both previously described and undescribed respiratory arsenate reduction potential via arrA was detected in Betaproteobacteria, Deltaproteobacteria, and Nitrospirae MAGs, whereas sulfate reduction potential was primarily limited to members of the Deltaproteobacteria and Nitrospirae. Lastly, iron reduction potential was detected in the Ignavibacteria, Deltaproteobacteria, and Nitrospirae. These results expand the phylogenetic distribution of taxa that may play roles in arsenic mobilization in subsurface systems. Specifically, the Nitrospirae are a much more functionally diverse group than previously assumed and may play key biogeochemical roles in arsenic-contaminated ecosystems. Public Library of Science 2019-09-06 /pmc/articles/PMC6730927/ /pubmed/31490939 http://dx.doi.org/10.1371/journal.pone.0221694 Text en © 2019 Danczak et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Danczak, Robert E.
Johnston, Michael D.
Kenah, Chris
Slattery, Michael
Wilkins, Michael J.
Capability for arsenic mobilization in groundwater is distributed across broad phylogenetic lineages
title Capability for arsenic mobilization in groundwater is distributed across broad phylogenetic lineages
title_full Capability for arsenic mobilization in groundwater is distributed across broad phylogenetic lineages
title_fullStr Capability for arsenic mobilization in groundwater is distributed across broad phylogenetic lineages
title_full_unstemmed Capability for arsenic mobilization in groundwater is distributed across broad phylogenetic lineages
title_short Capability for arsenic mobilization in groundwater is distributed across broad phylogenetic lineages
title_sort capability for arsenic mobilization in groundwater is distributed across broad phylogenetic lineages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6730927/
https://www.ncbi.nlm.nih.gov/pubmed/31490939
http://dx.doi.org/10.1371/journal.pone.0221694
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