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Cellular responses to beating hydrogels to investigate mechanotransduction

Cells feel the forces exerted on them by the surrounding extracellular matrix (ECM) environment and respond to them. While many cell fate processes are dictated by these forces, which are highly synchronized in space and time, abnormal force transduction is implicated in the progression of many dise...

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Autores principales: Chandorkar, Yashoda, Castro Nava, Arturo, Schweizerhof, Sjören, Van Dongen, Marcel, Haraszti, Tamás, Köhler, Jens, Zhang, Hang, Windoffer, Reinhard, Mourran, Ahmed, Möller, Martin, De Laporte, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6731269/
https://www.ncbi.nlm.nih.gov/pubmed/31492837
http://dx.doi.org/10.1038/s41467-019-11475-4
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author Chandorkar, Yashoda
Castro Nava, Arturo
Schweizerhof, Sjören
Van Dongen, Marcel
Haraszti, Tamás
Köhler, Jens
Zhang, Hang
Windoffer, Reinhard
Mourran, Ahmed
Möller, Martin
De Laporte, Laura
author_facet Chandorkar, Yashoda
Castro Nava, Arturo
Schweizerhof, Sjören
Van Dongen, Marcel
Haraszti, Tamás
Köhler, Jens
Zhang, Hang
Windoffer, Reinhard
Mourran, Ahmed
Möller, Martin
De Laporte, Laura
author_sort Chandorkar, Yashoda
collection PubMed
description Cells feel the forces exerted on them by the surrounding extracellular matrix (ECM) environment and respond to them. While many cell fate processes are dictated by these forces, which are highly synchronized in space and time, abnormal force transduction is implicated in the progression of many diseases (muscular dystrophy, cancer). However, material platforms that enable transient, cyclic forces in vitro to recreate an in vivo-like scenario remain a challenge. Here, we report a hydrogel system that rapidly beats (actuates) with spatio-temporal control using a near infra-red light trigger. Small, user-defined mechanical forces (~nN) are exerted on cells growing on the hydrogel surface at frequencies up to 10 Hz, revealing insights into the effect of actuation on cell migration and the kinetics of reversible nuclear translocation of the mechanosensor protein myocardin related transcription factor A, depending on the actuation amplitude, duration and frequency.
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spelling pubmed-67312692019-09-09 Cellular responses to beating hydrogels to investigate mechanotransduction Chandorkar, Yashoda Castro Nava, Arturo Schweizerhof, Sjören Van Dongen, Marcel Haraszti, Tamás Köhler, Jens Zhang, Hang Windoffer, Reinhard Mourran, Ahmed Möller, Martin De Laporte, Laura Nat Commun Article Cells feel the forces exerted on them by the surrounding extracellular matrix (ECM) environment and respond to them. While many cell fate processes are dictated by these forces, which are highly synchronized in space and time, abnormal force transduction is implicated in the progression of many diseases (muscular dystrophy, cancer). However, material platforms that enable transient, cyclic forces in vitro to recreate an in vivo-like scenario remain a challenge. Here, we report a hydrogel system that rapidly beats (actuates) with spatio-temporal control using a near infra-red light trigger. Small, user-defined mechanical forces (~nN) are exerted on cells growing on the hydrogel surface at frequencies up to 10 Hz, revealing insights into the effect of actuation on cell migration and the kinetics of reversible nuclear translocation of the mechanosensor protein myocardin related transcription factor A, depending on the actuation amplitude, duration and frequency. Nature Publishing Group UK 2019-09-06 /pmc/articles/PMC6731269/ /pubmed/31492837 http://dx.doi.org/10.1038/s41467-019-11475-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chandorkar, Yashoda
Castro Nava, Arturo
Schweizerhof, Sjören
Van Dongen, Marcel
Haraszti, Tamás
Köhler, Jens
Zhang, Hang
Windoffer, Reinhard
Mourran, Ahmed
Möller, Martin
De Laporte, Laura
Cellular responses to beating hydrogels to investigate mechanotransduction
title Cellular responses to beating hydrogels to investigate mechanotransduction
title_full Cellular responses to beating hydrogels to investigate mechanotransduction
title_fullStr Cellular responses to beating hydrogels to investigate mechanotransduction
title_full_unstemmed Cellular responses to beating hydrogels to investigate mechanotransduction
title_short Cellular responses to beating hydrogels to investigate mechanotransduction
title_sort cellular responses to beating hydrogels to investigate mechanotransduction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6731269/
https://www.ncbi.nlm.nih.gov/pubmed/31492837
http://dx.doi.org/10.1038/s41467-019-11475-4
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