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CRISPR-Cas9-based mutagenesis frequently provokes on-target mRNA misregulation

The introduction of insertion-deletions (INDELs) by non-homologous end-joining (NHEJ) pathway underlies the mechanistic basis of CRISPR-Cas9-directed genome editing. Selective gene ablation using CRISPR-Cas9 is achieved by installation of a premature termination codon (PTC) from a frameshift-inducin...

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Detalles Bibliográficos
Autores principales:	Tuladhar, Rubina, Yeu, Yunku, Tyler Piazza, John, Tan, Zhen, Rene Clemenceau, Jean, Wu, Xiaofeng, Barrett, Quinn, Herbert, Jeremiah, Mathews, David H., Kim, James, Hyun Hwang, Tae, Lum, Lawrence
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6731291/ https://www.ncbi.nlm.nih.gov/pubmed/31492834 http://dx.doi.org/10.1038/s41467-019-12028-5

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