Cargando…
Sentinel Lymph Node Analysis in Colorectal Cancer Patients Using One-Step Nucleic Acid Amplification in Combination With Fluorescence and Indocyanine Green
PURPOSE: Analysis of the sentinel lymph node (SLN) in colorectal cancer (CRC) patients was proposed for more accurate staging and tailored lymphadenectomy. The aim of this study was to assess the ability to predict lymph node (LN) involvement through analysis of the SLN with a one-step nucleic acid...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Coloproctology
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732328/ https://www.ncbi.nlm.nih.gov/pubmed/31487764 http://dx.doi.org/10.3393/ac.2018.07.21.1 |
_version_ | 1783449804463931392 |
---|---|
author | Esposito, Francesco Noviello, Adele Moles, Nicola Coppola Bottazzi, Enrico Baiamonte, Mario Macaione, Ina Ferbo, Umberto Lepore, Maria Miro, Antonio Crafa, Francesco |
author_facet | Esposito, Francesco Noviello, Adele Moles, Nicola Coppola Bottazzi, Enrico Baiamonte, Mario Macaione, Ina Ferbo, Umberto Lepore, Maria Miro, Antonio Crafa, Francesco |
author_sort | Esposito, Francesco |
collection | PubMed |
description | PURPOSE: Analysis of the sentinel lymph node (SLN) in colorectal cancer (CRC) patients was proposed for more accurate staging and tailored lymphadenectomy. The aim of this study was to assess the ability to predict lymph node (LN) involvement through analysis of the SLN with a one-step nucleic acid (OSNA) technique in combination with peritumoral injection of indocyanine green (ICG) and near-infrared (NIR) lymphangiography in CRC patients. METHODS: A total of 34 patients were enrolled. Overall, 51 LNs were analyzed with OSNA. LNs of 17 patients (50%) were examined simultaneously with hematoxylin and eosin (H&E) and OSNA. RESULTS: SLN analysis of 17 patients examined with H&E and OSNA revealed that OSNA had a higher sensitivity (1 vs. 0.55), higher negative predictive value (1 vs. 0.66) and higher accuracy (100% vs. 76.4%) in predicting LN involvement. Overall, OSNA showed a sensitivity of 0.69, specificity of 1, accuracy of 88.2%, and stage migration of 8.8%. Compared to those who were OSNA (−), OSNA (+) patients had a greater number of LN metastases (4.8 vs. 0.16, P = 0.04), higher G3 rate (44.4% vs. 4%, P = 0.01), more advanced stage of disease (stage III: 77.8% vs. 16%; P = 0.00) and were more rapidly subjected to adjuvant chemotherapy (39.1 days vs. 50.2 days, P = 0.01). CONCLUSION: SLN analysis with OSNA in combination with ICG-NIR lymphangiography is feasible and can detect LN involvement in CRC patients. Furthermore, it allows for more accurate staging reducing the delay between surgery and adjuvant chemotherapy. |
format | Online Article Text |
id | pubmed-6732328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Korean Society of Coloproctology |
record_format | MEDLINE/PubMed |
spelling | pubmed-67323282019-09-12 Sentinel Lymph Node Analysis in Colorectal Cancer Patients Using One-Step Nucleic Acid Amplification in Combination With Fluorescence and Indocyanine Green Esposito, Francesco Noviello, Adele Moles, Nicola Coppola Bottazzi, Enrico Baiamonte, Mario Macaione, Ina Ferbo, Umberto Lepore, Maria Miro, Antonio Crafa, Francesco Ann Coloproctol Original Article PURPOSE: Analysis of the sentinel lymph node (SLN) in colorectal cancer (CRC) patients was proposed for more accurate staging and tailored lymphadenectomy. The aim of this study was to assess the ability to predict lymph node (LN) involvement through analysis of the SLN with a one-step nucleic acid (OSNA) technique in combination with peritumoral injection of indocyanine green (ICG) and near-infrared (NIR) lymphangiography in CRC patients. METHODS: A total of 34 patients were enrolled. Overall, 51 LNs were analyzed with OSNA. LNs of 17 patients (50%) were examined simultaneously with hematoxylin and eosin (H&E) and OSNA. RESULTS: SLN analysis of 17 patients examined with H&E and OSNA revealed that OSNA had a higher sensitivity (1 vs. 0.55), higher negative predictive value (1 vs. 0.66) and higher accuracy (100% vs. 76.4%) in predicting LN involvement. Overall, OSNA showed a sensitivity of 0.69, specificity of 1, accuracy of 88.2%, and stage migration of 8.8%. Compared to those who were OSNA (−), OSNA (+) patients had a greater number of LN metastases (4.8 vs. 0.16, P = 0.04), higher G3 rate (44.4% vs. 4%, P = 0.01), more advanced stage of disease (stage III: 77.8% vs. 16%; P = 0.00) and were more rapidly subjected to adjuvant chemotherapy (39.1 days vs. 50.2 days, P = 0.01). CONCLUSION: SLN analysis with OSNA in combination with ICG-NIR lymphangiography is feasible and can detect LN involvement in CRC patients. Furthermore, it allows for more accurate staging reducing the delay between surgery and adjuvant chemotherapy. Korean Society of Coloproctology 2019-08 2019-08-31 /pmc/articles/PMC6732328/ /pubmed/31487764 http://dx.doi.org/10.3393/ac.2018.07.21.1 Text en Copyright © 2019 The Korean Society of Coloproctology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Esposito, Francesco Noviello, Adele Moles, Nicola Coppola Bottazzi, Enrico Baiamonte, Mario Macaione, Ina Ferbo, Umberto Lepore, Maria Miro, Antonio Crafa, Francesco Sentinel Lymph Node Analysis in Colorectal Cancer Patients Using One-Step Nucleic Acid Amplification in Combination With Fluorescence and Indocyanine Green |
title | Sentinel Lymph Node Analysis in Colorectal Cancer Patients Using One-Step Nucleic Acid Amplification in Combination With Fluorescence and Indocyanine Green |
title_full | Sentinel Lymph Node Analysis in Colorectal Cancer Patients Using One-Step Nucleic Acid Amplification in Combination With Fluorescence and Indocyanine Green |
title_fullStr | Sentinel Lymph Node Analysis in Colorectal Cancer Patients Using One-Step Nucleic Acid Amplification in Combination With Fluorescence and Indocyanine Green |
title_full_unstemmed | Sentinel Lymph Node Analysis in Colorectal Cancer Patients Using One-Step Nucleic Acid Amplification in Combination With Fluorescence and Indocyanine Green |
title_short | Sentinel Lymph Node Analysis in Colorectal Cancer Patients Using One-Step Nucleic Acid Amplification in Combination With Fluorescence and Indocyanine Green |
title_sort | sentinel lymph node analysis in colorectal cancer patients using one-step nucleic acid amplification in combination with fluorescence and indocyanine green |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732328/ https://www.ncbi.nlm.nih.gov/pubmed/31487764 http://dx.doi.org/10.3393/ac.2018.07.21.1 |
work_keys_str_mv | AT espositofrancesco sentinellymphnodeanalysisincolorectalcancerpatientsusingonestepnucleicacidamplificationincombinationwithfluorescenceandindocyaninegreen AT novielloadele sentinellymphnodeanalysisincolorectalcancerpatientsusingonestepnucleicacidamplificationincombinationwithfluorescenceandindocyaninegreen AT molesnicola sentinellymphnodeanalysisincolorectalcancerpatientsusingonestepnucleicacidamplificationincombinationwithfluorescenceandindocyaninegreen AT coppolabottazzienrico sentinellymphnodeanalysisincolorectalcancerpatientsusingonestepnucleicacidamplificationincombinationwithfluorescenceandindocyaninegreen AT baiamontemario sentinellymphnodeanalysisincolorectalcancerpatientsusingonestepnucleicacidamplificationincombinationwithfluorescenceandindocyaninegreen AT macaioneina sentinellymphnodeanalysisincolorectalcancerpatientsusingonestepnucleicacidamplificationincombinationwithfluorescenceandindocyaninegreen AT ferboumberto sentinellymphnodeanalysisincolorectalcancerpatientsusingonestepnucleicacidamplificationincombinationwithfluorescenceandindocyaninegreen AT leporemaria sentinellymphnodeanalysisincolorectalcancerpatientsusingonestepnucleicacidamplificationincombinationwithfluorescenceandindocyaninegreen AT miroantonio sentinellymphnodeanalysisincolorectalcancerpatientsusingonestepnucleicacidamplificationincombinationwithfluorescenceandindocyaninegreen AT crafafrancesco sentinellymphnodeanalysisincolorectalcancerpatientsusingonestepnucleicacidamplificationincombinationwithfluorescenceandindocyaninegreen |