Cargando…

Effect of curcumin on gene expression and protein level of methionine sulfoxide reductase A (MSRA), SOD, CAT and GPx in Freund’s adjuvant inflammation-induced male rats

OBJECTIVE: Curcumin is the well-known compound which is extracted from turmeric powder, the dried rhizome of the Curcuma longa Linn. This have been used for the treatment of various disorders including inflammation. In this study we have analyzed the effect of curcumin on arthritis induced by adjuva...

Descripción completa

Detalles Bibliográficos
Autores principales: Meshkibaf, MH, Maleknia, M, Noroozi, S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732743/
https://www.ncbi.nlm.nih.gov/pubmed/31564949
http://dx.doi.org/10.2147/JIR.S212577
_version_ 1783449866008002560
author Meshkibaf, MH
Maleknia, M
Noroozi, S
author_facet Meshkibaf, MH
Maleknia, M
Noroozi, S
author_sort Meshkibaf, MH
collection PubMed
description OBJECTIVE: Curcumin is the well-known compound which is extracted from turmeric powder, the dried rhizome of the Curcuma longa Linn. This have been used for the treatment of various disorders including inflammation. In this study we have analyzed the effect of curcumin on arthritis induced by adjuvant in rats, considering changes in methionine sulfoxide reductase A (MSRA) expression and antioxidant enzymes levels. METHODS: Five groups of adult male Wistar rats (n=10), were randomly selected as control, placebo, experimental 1, 2 and 3. The induction of arthritis was carried out by injection of 0.1 ml adjuvant in plantar region. The first experimental group received no curcumin treatment, whereas the experimental two and three received curcumin (1 and 2 g/kg daily) respectively, for fourteen days. MSRA gene expression was assessed by real-time PCR and protein levels of MSRA, SOD, CAT and GPx were analyzed via ELISA method. RESULTS: The results showed no significant weight changes among the groups during the experimental period and the paw swelling caused by adjuvant was recovered within fourteen days of treatment with curcumin. However, the levels of enzymes such as superoxide dismutase, catalase and glutathione peroxidase were increased by a dose dependent manner. These results also illustrated that the gene expression and protein level of MSRA in groups treated with curcumin increased significantly (p≤0.05). CONCLUSION: We concluded that the curcumin can be used against inflammation. The increasing level of MSRA can be due to the antioxidant effect of curcumin. The enzymatic level changes (MSRA, SOD, CAT and GPx) may interfere with the aging process and delay it.
format Online
Article
Text
id pubmed-6732743
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-67327432019-09-27 Effect of curcumin on gene expression and protein level of methionine sulfoxide reductase A (MSRA), SOD, CAT and GPx in Freund’s adjuvant inflammation-induced male rats Meshkibaf, MH Maleknia, M Noroozi, S J Inflamm Res Original Research OBJECTIVE: Curcumin is the well-known compound which is extracted from turmeric powder, the dried rhizome of the Curcuma longa Linn. This have been used for the treatment of various disorders including inflammation. In this study we have analyzed the effect of curcumin on arthritis induced by adjuvant in rats, considering changes in methionine sulfoxide reductase A (MSRA) expression and antioxidant enzymes levels. METHODS: Five groups of adult male Wistar rats (n=10), were randomly selected as control, placebo, experimental 1, 2 and 3. The induction of arthritis was carried out by injection of 0.1 ml adjuvant in plantar region. The first experimental group received no curcumin treatment, whereas the experimental two and three received curcumin (1 and 2 g/kg daily) respectively, for fourteen days. MSRA gene expression was assessed by real-time PCR and protein levels of MSRA, SOD, CAT and GPx were analyzed via ELISA method. RESULTS: The results showed no significant weight changes among the groups during the experimental period and the paw swelling caused by adjuvant was recovered within fourteen days of treatment with curcumin. However, the levels of enzymes such as superoxide dismutase, catalase and glutathione peroxidase were increased by a dose dependent manner. These results also illustrated that the gene expression and protein level of MSRA in groups treated with curcumin increased significantly (p≤0.05). CONCLUSION: We concluded that the curcumin can be used against inflammation. The increasing level of MSRA can be due to the antioxidant effect of curcumin. The enzymatic level changes (MSRA, SOD, CAT and GPx) may interfere with the aging process and delay it. Dove 2019-09-03 /pmc/articles/PMC6732743/ /pubmed/31564949 http://dx.doi.org/10.2147/JIR.S212577 Text en © 2019 Meshkibaf et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Meshkibaf, MH
Maleknia, M
Noroozi, S
Effect of curcumin on gene expression and protein level of methionine sulfoxide reductase A (MSRA), SOD, CAT and GPx in Freund’s adjuvant inflammation-induced male rats
title Effect of curcumin on gene expression and protein level of methionine sulfoxide reductase A (MSRA), SOD, CAT and GPx in Freund’s adjuvant inflammation-induced male rats
title_full Effect of curcumin on gene expression and protein level of methionine sulfoxide reductase A (MSRA), SOD, CAT and GPx in Freund’s adjuvant inflammation-induced male rats
title_fullStr Effect of curcumin on gene expression and protein level of methionine sulfoxide reductase A (MSRA), SOD, CAT and GPx in Freund’s adjuvant inflammation-induced male rats
title_full_unstemmed Effect of curcumin on gene expression and protein level of methionine sulfoxide reductase A (MSRA), SOD, CAT and GPx in Freund’s adjuvant inflammation-induced male rats
title_short Effect of curcumin on gene expression and protein level of methionine sulfoxide reductase A (MSRA), SOD, CAT and GPx in Freund’s adjuvant inflammation-induced male rats
title_sort effect of curcumin on gene expression and protein level of methionine sulfoxide reductase a (msra), sod, cat and gpx in freund’s adjuvant inflammation-induced male rats
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732743/
https://www.ncbi.nlm.nih.gov/pubmed/31564949
http://dx.doi.org/10.2147/JIR.S212577
work_keys_str_mv AT meshkibafmh effectofcurcuminongeneexpressionandproteinlevelofmethioninesulfoxidereductaseamsrasodcatandgpxinfreundsadjuvantinflammationinducedmalerats
AT malekniam effectofcurcuminongeneexpressionandproteinlevelofmethioninesulfoxidereductaseamsrasodcatandgpxinfreundsadjuvantinflammationinducedmalerats
AT noroozis effectofcurcuminongeneexpressionandproteinlevelofmethioninesulfoxidereductaseamsrasodcatandgpxinfreundsadjuvantinflammationinducedmalerats