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Human Circadian Molecular Oscillation Development Using Induced Pluripotent Stem Cells

The mammalian circadian clock, which coordinates various physiological functions, develops gradually during ontogeny. Recently, we have reported the posttranscriptional suppression of CLOCK protein expression as a key mechanism of the emergence of the circadian clock during mouse development. Howeve...

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Autores principales: Umemura, Yasuhiro, Maki, Izumi, Tsuchiya, Yoshiki, Koike, Nobuya, Yagita, Kazuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732938/
https://www.ncbi.nlm.nih.gov/pubmed/31368392
http://dx.doi.org/10.1177/0748730419865436
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author Umemura, Yasuhiro
Maki, Izumi
Tsuchiya, Yoshiki
Koike, Nobuya
Yagita, Kazuhiro
author_facet Umemura, Yasuhiro
Maki, Izumi
Tsuchiya, Yoshiki
Koike, Nobuya
Yagita, Kazuhiro
author_sort Umemura, Yasuhiro
collection PubMed
description The mammalian circadian clock, which coordinates various physiological functions, develops gradually during ontogeny. Recently, we have reported the posttranscriptional suppression of CLOCK protein expression as a key mechanism of the emergence of the circadian clock during mouse development. However, whether a common mechanism regulates the development of the human circadian clock remains unclear. In the present study, we show that human induced pluripotent stem cells (iPSCs) have no discernible circadian molecular oscillation. In addition, in vitro differentiation culture of human iPSCs required a longer duration than that required in mouse for the emergence of circadian oscillations. The expression of CLOCK protein in undifferentiated human iPSCs was posttranscriptionally suppressed despite the expression of CLOCK mRNA, which is consistent with our previous observations in mouse embryonic stem cells, iPSCs, and early mouse embryos. These results suggest that CLOCK protein expressions could be posttranscriptionally suppressed in the early developmental stage not only in mice but also in humans.
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spelling pubmed-67329382019-10-03 Human Circadian Molecular Oscillation Development Using Induced Pluripotent Stem Cells Umemura, Yasuhiro Maki, Izumi Tsuchiya, Yoshiki Koike, Nobuya Yagita, Kazuhiro J Biol Rhythms Original Articles The mammalian circadian clock, which coordinates various physiological functions, develops gradually during ontogeny. Recently, we have reported the posttranscriptional suppression of CLOCK protein expression as a key mechanism of the emergence of the circadian clock during mouse development. However, whether a common mechanism regulates the development of the human circadian clock remains unclear. In the present study, we show that human induced pluripotent stem cells (iPSCs) have no discernible circadian molecular oscillation. In addition, in vitro differentiation culture of human iPSCs required a longer duration than that required in mouse for the emergence of circadian oscillations. The expression of CLOCK protein in undifferentiated human iPSCs was posttranscriptionally suppressed despite the expression of CLOCK mRNA, which is consistent with our previous observations in mouse embryonic stem cells, iPSCs, and early mouse embryos. These results suggest that CLOCK protein expressions could be posttranscriptionally suppressed in the early developmental stage not only in mice but also in humans. SAGE Publications 2019-08-01 2019-10 /pmc/articles/PMC6732938/ /pubmed/31368392 http://dx.doi.org/10.1177/0748730419865436 Text en © 2019 The Author(s) http://www.creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Articles
Umemura, Yasuhiro
Maki, Izumi
Tsuchiya, Yoshiki
Koike, Nobuya
Yagita, Kazuhiro
Human Circadian Molecular Oscillation Development Using Induced Pluripotent Stem Cells
title Human Circadian Molecular Oscillation Development Using Induced Pluripotent Stem Cells
title_full Human Circadian Molecular Oscillation Development Using Induced Pluripotent Stem Cells
title_fullStr Human Circadian Molecular Oscillation Development Using Induced Pluripotent Stem Cells
title_full_unstemmed Human Circadian Molecular Oscillation Development Using Induced Pluripotent Stem Cells
title_short Human Circadian Molecular Oscillation Development Using Induced Pluripotent Stem Cells
title_sort human circadian molecular oscillation development using induced pluripotent stem cells
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732938/
https://www.ncbi.nlm.nih.gov/pubmed/31368392
http://dx.doi.org/10.1177/0748730419865436
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