Cargando…
LncRNA AWPPH participates in the metastasis of non-small cell lung cancer by upregulating TGF-β1 expression
Long non-coding RNA (lncRNA) AWPPH is a characterized oncogenic lncRNA in hepatocellular carcinoma and bladder cancer. The aim of the present study was to investigate the potential involvement of AWPPH in non-small cell lung cancer (NSCLC). Expression of AWPPH in lung biopsy tissues and serum of bot...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732945/ https://www.ncbi.nlm.nih.gov/pubmed/31516619 http://dx.doi.org/10.3892/ol.2019.10754 |
_version_ | 1783449894540804096 |
---|---|
author | Huo, Yanxia Li, Aimin Wang, Zhihua |
author_facet | Huo, Yanxia Li, Aimin Wang, Zhihua |
author_sort | Huo, Yanxia |
collection | PubMed |
description | Long non-coding RNA (lncRNA) AWPPH is a characterized oncogenic lncRNA in hepatocellular carcinoma and bladder cancer. The aim of the present study was to investigate the potential involvement of AWPPH in non-small cell lung cancer (NSCLC). Expression of AWPPH in lung biopsy tissues and serum of both healthy controls and patients with NSCLC with different tumor sizes, and with and without distant tumor metastasis was detected by reverse transcription-quantitative PCR. AWPPH expression vector was constructed and transfected into cells of human NSCLC cell lines. Expression of TGF-β1 was detected by western blot analysis. Cell migration and invasion were detected by Transwell migration and invasion assay. It was observed that AWPPH was significantly upregulated in patients with NSCLC, while AWPPH expression level did not increase with the increase of tumor size. In contrast, AWPPH expression levels were significantly higher in patients with distant metastasis than in patients without tumor distant metastasis. AWPPH overexpression promoted TGF-β1 expression in NSCLC cells, while TGF-β1 treatment showed no significant effects on AWPPH expression. AWPPH overexpression promoted NSCLC cell migration and invasion, while TGF-β signaling inhibition reduced this enhancing effect. Therefore, AWPPH may promote the metastasis, but not the growth of NSCLC by upregulating TGF-β1 expression. |
format | Online Article Text |
id | pubmed-6732945 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-67329452019-09-12 LncRNA AWPPH participates in the metastasis of non-small cell lung cancer by upregulating TGF-β1 expression Huo, Yanxia Li, Aimin Wang, Zhihua Oncol Lett Articles Long non-coding RNA (lncRNA) AWPPH is a characterized oncogenic lncRNA in hepatocellular carcinoma and bladder cancer. The aim of the present study was to investigate the potential involvement of AWPPH in non-small cell lung cancer (NSCLC). Expression of AWPPH in lung biopsy tissues and serum of both healthy controls and patients with NSCLC with different tumor sizes, and with and without distant tumor metastasis was detected by reverse transcription-quantitative PCR. AWPPH expression vector was constructed and transfected into cells of human NSCLC cell lines. Expression of TGF-β1 was detected by western blot analysis. Cell migration and invasion were detected by Transwell migration and invasion assay. It was observed that AWPPH was significantly upregulated in patients with NSCLC, while AWPPH expression level did not increase with the increase of tumor size. In contrast, AWPPH expression levels were significantly higher in patients with distant metastasis than in patients without tumor distant metastasis. AWPPH overexpression promoted TGF-β1 expression in NSCLC cells, while TGF-β1 treatment showed no significant effects on AWPPH expression. AWPPH overexpression promoted NSCLC cell migration and invasion, while TGF-β signaling inhibition reduced this enhancing effect. Therefore, AWPPH may promote the metastasis, but not the growth of NSCLC by upregulating TGF-β1 expression. D.A. Spandidos 2019-10 2019-08-16 /pmc/articles/PMC6732945/ /pubmed/31516619 http://dx.doi.org/10.3892/ol.2019.10754 Text en Copyright: © Huo et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Huo, Yanxia Li, Aimin Wang, Zhihua LncRNA AWPPH participates in the metastasis of non-small cell lung cancer by upregulating TGF-β1 expression |
title | LncRNA AWPPH participates in the metastasis of non-small cell lung cancer by upregulating TGF-β1 expression |
title_full | LncRNA AWPPH participates in the metastasis of non-small cell lung cancer by upregulating TGF-β1 expression |
title_fullStr | LncRNA AWPPH participates in the metastasis of non-small cell lung cancer by upregulating TGF-β1 expression |
title_full_unstemmed | LncRNA AWPPH participates in the metastasis of non-small cell lung cancer by upregulating TGF-β1 expression |
title_short | LncRNA AWPPH participates in the metastasis of non-small cell lung cancer by upregulating TGF-β1 expression |
title_sort | lncrna awpph participates in the metastasis of non-small cell lung cancer by upregulating tgf-β1 expression |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732945/ https://www.ncbi.nlm.nih.gov/pubmed/31516619 http://dx.doi.org/10.3892/ol.2019.10754 |
work_keys_str_mv | AT huoyanxia lncrnaawpphparticipatesinthemetastasisofnonsmallcelllungcancerbyupregulatingtgfb1expression AT liaimin lncrnaawpphparticipatesinthemetastasisofnonsmallcelllungcancerbyupregulatingtgfb1expression AT wangzhihua lncrnaawpphparticipatesinthemetastasisofnonsmallcelllungcancerbyupregulatingtgfb1expression |