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TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells

Despite the canonical homogeneous character of its organization, the cerebellum plays differential computational roles in distinct sensorimotor behaviors. Previously, we showed that Purkinje cell (PC) activity differs between zebrin-negative (Z–) and zebrin-positive (Z+) modules (Zhou et al., 2014)....

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Autores principales: Wu, Bin, Blot, François GC, Wong, Aaron Benson, Osório, Catarina, Adolfs, Youri, Pasterkamp, R Jeroen, Hartmann, Jana, Becker, Esther BE, Boele, Henk-Jan, De Zeeuw, Chris I, Schonewille, Martijn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6733575/
https://www.ncbi.nlm.nih.gov/pubmed/31486767
http://dx.doi.org/10.7554/eLife.45590
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author Wu, Bin
Blot, François GC
Wong, Aaron Benson
Osório, Catarina
Adolfs, Youri
Pasterkamp, R Jeroen
Hartmann, Jana
Becker, Esther BE
Boele, Henk-Jan
De Zeeuw, Chris I
Schonewille, Martijn
author_facet Wu, Bin
Blot, François GC
Wong, Aaron Benson
Osório, Catarina
Adolfs, Youri
Pasterkamp, R Jeroen
Hartmann, Jana
Becker, Esther BE
Boele, Henk-Jan
De Zeeuw, Chris I
Schonewille, Martijn
author_sort Wu, Bin
collection PubMed
description Despite the canonical homogeneous character of its organization, the cerebellum plays differential computational roles in distinct sensorimotor behaviors. Previously, we showed that Purkinje cell (PC) activity differs between zebrin-negative (Z–) and zebrin-positive (Z+) modules (Zhou et al., 2014). Here, using gain-of-function and loss-of-function mouse models, we show that transient receptor potential cation channel C3 (TRPC3) controls the simple spike activity of Z–, but not Z+ PCs. In addition, TRPC3 regulates complex spike rate and their interaction with simple spikes, exclusively in Z– PCs. At the behavioral level, TRPC3 loss-of-function mice show impaired eyeblink conditioning, which is related to Z– modules, whereas compensatory eye movement adaptation, linked to Z+ modules, is intact. Together, our results indicate that TRPC3 is a major contributor to the cellular heterogeneity that introduces distinct physiological properties in PCs, conjuring functional heterogeneity in cerebellar sensorimotor integration.
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spelling pubmed-67335752019-09-11 TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells Wu, Bin Blot, François GC Wong, Aaron Benson Osório, Catarina Adolfs, Youri Pasterkamp, R Jeroen Hartmann, Jana Becker, Esther BE Boele, Henk-Jan De Zeeuw, Chris I Schonewille, Martijn eLife Neuroscience Despite the canonical homogeneous character of its organization, the cerebellum plays differential computational roles in distinct sensorimotor behaviors. Previously, we showed that Purkinje cell (PC) activity differs between zebrin-negative (Z–) and zebrin-positive (Z+) modules (Zhou et al., 2014). Here, using gain-of-function and loss-of-function mouse models, we show that transient receptor potential cation channel C3 (TRPC3) controls the simple spike activity of Z–, but not Z+ PCs. In addition, TRPC3 regulates complex spike rate and their interaction with simple spikes, exclusively in Z– PCs. At the behavioral level, TRPC3 loss-of-function mice show impaired eyeblink conditioning, which is related to Z– modules, whereas compensatory eye movement adaptation, linked to Z+ modules, is intact. Together, our results indicate that TRPC3 is a major contributor to the cellular heterogeneity that introduces distinct physiological properties in PCs, conjuring functional heterogeneity in cerebellar sensorimotor integration. eLife Sciences Publications, Ltd 2019-09-05 /pmc/articles/PMC6733575/ /pubmed/31486767 http://dx.doi.org/10.7554/eLife.45590 Text en © 2019, Wu et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Wu, Bin
Blot, François GC
Wong, Aaron Benson
Osório, Catarina
Adolfs, Youri
Pasterkamp, R Jeroen
Hartmann, Jana
Becker, Esther BE
Boele, Henk-Jan
De Zeeuw, Chris I
Schonewille, Martijn
TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells
title TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells
title_full TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells
title_fullStr TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells
title_full_unstemmed TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells
title_short TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells
title_sort trpc3 is a major contributor to functional heterogeneity of cerebellar purkinje cells
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6733575/
https://www.ncbi.nlm.nih.gov/pubmed/31486767
http://dx.doi.org/10.7554/eLife.45590
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