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TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells
Despite the canonical homogeneous character of its organization, the cerebellum plays differential computational roles in distinct sensorimotor behaviors. Previously, we showed that Purkinje cell (PC) activity differs between zebrin-negative (Z–) and zebrin-positive (Z+) modules (Zhou et al., 2014)....
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6733575/ https://www.ncbi.nlm.nih.gov/pubmed/31486767 http://dx.doi.org/10.7554/eLife.45590 |
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author | Wu, Bin Blot, François GC Wong, Aaron Benson Osório, Catarina Adolfs, Youri Pasterkamp, R Jeroen Hartmann, Jana Becker, Esther BE Boele, Henk-Jan De Zeeuw, Chris I Schonewille, Martijn |
author_facet | Wu, Bin Blot, François GC Wong, Aaron Benson Osório, Catarina Adolfs, Youri Pasterkamp, R Jeroen Hartmann, Jana Becker, Esther BE Boele, Henk-Jan De Zeeuw, Chris I Schonewille, Martijn |
author_sort | Wu, Bin |
collection | PubMed |
description | Despite the canonical homogeneous character of its organization, the cerebellum plays differential computational roles in distinct sensorimotor behaviors. Previously, we showed that Purkinje cell (PC) activity differs between zebrin-negative (Z–) and zebrin-positive (Z+) modules (Zhou et al., 2014). Here, using gain-of-function and loss-of-function mouse models, we show that transient receptor potential cation channel C3 (TRPC3) controls the simple spike activity of Z–, but not Z+ PCs. In addition, TRPC3 regulates complex spike rate and their interaction with simple spikes, exclusively in Z– PCs. At the behavioral level, TRPC3 loss-of-function mice show impaired eyeblink conditioning, which is related to Z– modules, whereas compensatory eye movement adaptation, linked to Z+ modules, is intact. Together, our results indicate that TRPC3 is a major contributor to the cellular heterogeneity that introduces distinct physiological properties in PCs, conjuring functional heterogeneity in cerebellar sensorimotor integration. |
format | Online Article Text |
id | pubmed-6733575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-67335752019-09-11 TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells Wu, Bin Blot, François GC Wong, Aaron Benson Osório, Catarina Adolfs, Youri Pasterkamp, R Jeroen Hartmann, Jana Becker, Esther BE Boele, Henk-Jan De Zeeuw, Chris I Schonewille, Martijn eLife Neuroscience Despite the canonical homogeneous character of its organization, the cerebellum plays differential computational roles in distinct sensorimotor behaviors. Previously, we showed that Purkinje cell (PC) activity differs between zebrin-negative (Z–) and zebrin-positive (Z+) modules (Zhou et al., 2014). Here, using gain-of-function and loss-of-function mouse models, we show that transient receptor potential cation channel C3 (TRPC3) controls the simple spike activity of Z–, but not Z+ PCs. In addition, TRPC3 regulates complex spike rate and their interaction with simple spikes, exclusively in Z– PCs. At the behavioral level, TRPC3 loss-of-function mice show impaired eyeblink conditioning, which is related to Z– modules, whereas compensatory eye movement adaptation, linked to Z+ modules, is intact. Together, our results indicate that TRPC3 is a major contributor to the cellular heterogeneity that introduces distinct physiological properties in PCs, conjuring functional heterogeneity in cerebellar sensorimotor integration. eLife Sciences Publications, Ltd 2019-09-05 /pmc/articles/PMC6733575/ /pubmed/31486767 http://dx.doi.org/10.7554/eLife.45590 Text en © 2019, Wu et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Wu, Bin Blot, François GC Wong, Aaron Benson Osório, Catarina Adolfs, Youri Pasterkamp, R Jeroen Hartmann, Jana Becker, Esther BE Boele, Henk-Jan De Zeeuw, Chris I Schonewille, Martijn TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells |
title | TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells |
title_full | TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells |
title_fullStr | TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells |
title_full_unstemmed | TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells |
title_short | TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells |
title_sort | trpc3 is a major contributor to functional heterogeneity of cerebellar purkinje cells |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6733575/ https://www.ncbi.nlm.nih.gov/pubmed/31486767 http://dx.doi.org/10.7554/eLife.45590 |
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