Cargando…
Direct neuronal reprogramming of olfactory ensheathing cells for CNS repair
Direct conversion of readily available non-neural cells from patients into induced neurons holds great promise for neurological disease modeling and cell-based therapy. Olfactory ensheathing cells (OECs) is a unique population of glia in olfactory nervous system. Based on the regeneration-promoting...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6733847/ https://www.ncbi.nlm.nih.gov/pubmed/31501413 http://dx.doi.org/10.1038/s41419-019-1887-4 |
_version_ | 1783450037663039488 |
---|---|
author | Sun, Xiu Tan, Zijian Huang, Xiao Cheng, Xueyan Yuan, Yimin Qin, Shangyao Wang, Dan Hu, Xin Gu, Yakun Qian, Wen-Jing Wang, Zhongfeng He, Cheng Su, Zhida |
author_facet | Sun, Xiu Tan, Zijian Huang, Xiao Cheng, Xueyan Yuan, Yimin Qin, Shangyao Wang, Dan Hu, Xin Gu, Yakun Qian, Wen-Jing Wang, Zhongfeng He, Cheng Su, Zhida |
author_sort | Sun, Xiu |
collection | PubMed |
description | Direct conversion of readily available non-neural cells from patients into induced neurons holds great promise for neurological disease modeling and cell-based therapy. Olfactory ensheathing cells (OECs) is a unique population of glia in olfactory nervous system. Based on the regeneration-promoting properties and the relative clinical accessibility, OECs are attracting increasing attention from neuroscientists as potential therapeutic agents for use in neural repair. Here, we report that OECs can be directly, rapidly and efficiently reprogrammed into neuronal cells by the single transcription factor Neurogenin 2 (NGN2). These induced cells exhibit typical neuronal morphologies, express multiple neuron-specific markers, produce action potentials, and form functional synapses. Genome-wide RNA-sequencing analysis shows that the transcriptome profile of OECs is effectively reprogrammed towards that of neuronal lineage. Importantly, these OEC-derived induced neurons survive and mature after transplantation into adult mouse spinal cords. Taken together, our study provides a direct and efficient strategy to quickly obtain neuronal cells from adult OECs, suggestive of promising potential for personalized disease modeling and cell replacement-mediated therapeutic approaches to neurological disorders. |
format | Online Article Text |
id | pubmed-6733847 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67338472019-09-10 Direct neuronal reprogramming of olfactory ensheathing cells for CNS repair Sun, Xiu Tan, Zijian Huang, Xiao Cheng, Xueyan Yuan, Yimin Qin, Shangyao Wang, Dan Hu, Xin Gu, Yakun Qian, Wen-Jing Wang, Zhongfeng He, Cheng Su, Zhida Cell Death Dis Article Direct conversion of readily available non-neural cells from patients into induced neurons holds great promise for neurological disease modeling and cell-based therapy. Olfactory ensheathing cells (OECs) is a unique population of glia in olfactory nervous system. Based on the regeneration-promoting properties and the relative clinical accessibility, OECs are attracting increasing attention from neuroscientists as potential therapeutic agents for use in neural repair. Here, we report that OECs can be directly, rapidly and efficiently reprogrammed into neuronal cells by the single transcription factor Neurogenin 2 (NGN2). These induced cells exhibit typical neuronal morphologies, express multiple neuron-specific markers, produce action potentials, and form functional synapses. Genome-wide RNA-sequencing analysis shows that the transcriptome profile of OECs is effectively reprogrammed towards that of neuronal lineage. Importantly, these OEC-derived induced neurons survive and mature after transplantation into adult mouse spinal cords. Taken together, our study provides a direct and efficient strategy to quickly obtain neuronal cells from adult OECs, suggestive of promising potential for personalized disease modeling and cell replacement-mediated therapeutic approaches to neurological disorders. Nature Publishing Group UK 2019-09-09 /pmc/articles/PMC6733847/ /pubmed/31501413 http://dx.doi.org/10.1038/s41419-019-1887-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Sun, Xiu Tan, Zijian Huang, Xiao Cheng, Xueyan Yuan, Yimin Qin, Shangyao Wang, Dan Hu, Xin Gu, Yakun Qian, Wen-Jing Wang, Zhongfeng He, Cheng Su, Zhida Direct neuronal reprogramming of olfactory ensheathing cells for CNS repair |
title | Direct neuronal reprogramming of olfactory ensheathing cells for CNS repair |
title_full | Direct neuronal reprogramming of olfactory ensheathing cells for CNS repair |
title_fullStr | Direct neuronal reprogramming of olfactory ensheathing cells for CNS repair |
title_full_unstemmed | Direct neuronal reprogramming of olfactory ensheathing cells for CNS repair |
title_short | Direct neuronal reprogramming of olfactory ensheathing cells for CNS repair |
title_sort | direct neuronal reprogramming of olfactory ensheathing cells for cns repair |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6733847/ https://www.ncbi.nlm.nih.gov/pubmed/31501413 http://dx.doi.org/10.1038/s41419-019-1887-4 |
work_keys_str_mv | AT sunxiu directneuronalreprogrammingofolfactoryensheathingcellsforcnsrepair AT tanzijian directneuronalreprogrammingofolfactoryensheathingcellsforcnsrepair AT huangxiao directneuronalreprogrammingofolfactoryensheathingcellsforcnsrepair AT chengxueyan directneuronalreprogrammingofolfactoryensheathingcellsforcnsrepair AT yuanyimin directneuronalreprogrammingofolfactoryensheathingcellsforcnsrepair AT qinshangyao directneuronalreprogrammingofolfactoryensheathingcellsforcnsrepair AT wangdan directneuronalreprogrammingofolfactoryensheathingcellsforcnsrepair AT huxin directneuronalreprogrammingofolfactoryensheathingcellsforcnsrepair AT guyakun directneuronalreprogrammingofolfactoryensheathingcellsforcnsrepair AT qianwenjing directneuronalreprogrammingofolfactoryensheathingcellsforcnsrepair AT wangzhongfeng directneuronalreprogrammingofolfactoryensheathingcellsforcnsrepair AT hecheng directneuronalreprogrammingofolfactoryensheathingcellsforcnsrepair AT suzhida directneuronalreprogrammingofolfactoryensheathingcellsforcnsrepair |