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Graphene quantum dots: Synthesis, characterization, cell viability, genotoxicity for biomedical applications
We report the synthesis and applications of a novel N-doped graphene quantum dots (GQDs) using hydrothermal reaction between citric acid and p-aminophenol. The synthesized N-doped GQDs have been characterized physico-chemically and evaluated its antioxidant, antimicrobial, DNA binding and cleavage a...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6733895/ https://www.ncbi.nlm.nih.gov/pubmed/31516327 http://dx.doi.org/10.1016/j.jsps.2019.05.006 |
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author | Şenel, Behiye Demir, Neslihan Büyükköroğlu, Gülay Yıldız, Mustafa |
author_facet | Şenel, Behiye Demir, Neslihan Büyükköroğlu, Gülay Yıldız, Mustafa |
author_sort | Şenel, Behiye |
collection | PubMed |
description | We report the synthesis and applications of a novel N-doped graphene quantum dots (GQDs) using hydrothermal reaction between citric acid and p-aminophenol. The synthesized N-doped GQDs have been characterized physico-chemically and evaluated its antioxidant, antimicrobial, DNA binding and cleavage activities. siRNA loading studies were performed and their effects on cells were evaluated. Obtained results indicate that monodisperse solution of N-doped GQDs has been obtained with particles size ca. ∼10.9 ± 1.3 nm. UV–Vis spectroscopy studies of the interactions between the N-doped GQDs and calf thymus DNA (CT-DNA) showed that the compound interact with CT-DNA via both intercalative and electrostatic binding. The DNA cleavage study showed that the N-doped GQDs cleaved DNA without any external agents. The antioxidant activity of N-doped GQDS was very active when compared to BHT. As the concentration of the compound increased, the antioxidant activity also increased. Cell viability assay demonstrated that the Ndoped GQDs showed cell viability (70%) when the concentration reached 200 μg/mL for A549 and also MDA-MB-231, 150 μg/mL for NIH-3T3 cell lines at 24 h incubation. N-doped GQDs were coated with Eudragit RS 100 and EphA2-siRNA was loaded. As a result of the studies on these formulations, it was concluded that there may be significant effects on A549 cells. The microscopy results revealed that N-doped GQDs was quickly internalized into the cell. Our novel N-doped-GQDs with siRNA are candidate for in situ tumor suppression via DNA and mRNA breakage. |
format | Online Article Text |
id | pubmed-6733895 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-67338952019-09-12 Graphene quantum dots: Synthesis, characterization, cell viability, genotoxicity for biomedical applications Şenel, Behiye Demir, Neslihan Büyükköroğlu, Gülay Yıldız, Mustafa Saudi Pharm J Original Article We report the synthesis and applications of a novel N-doped graphene quantum dots (GQDs) using hydrothermal reaction between citric acid and p-aminophenol. The synthesized N-doped GQDs have been characterized physico-chemically and evaluated its antioxidant, antimicrobial, DNA binding and cleavage activities. siRNA loading studies were performed and their effects on cells were evaluated. Obtained results indicate that monodisperse solution of N-doped GQDs has been obtained with particles size ca. ∼10.9 ± 1.3 nm. UV–Vis spectroscopy studies of the interactions between the N-doped GQDs and calf thymus DNA (CT-DNA) showed that the compound interact with CT-DNA via both intercalative and electrostatic binding. The DNA cleavage study showed that the N-doped GQDs cleaved DNA without any external agents. The antioxidant activity of N-doped GQDS was very active when compared to BHT. As the concentration of the compound increased, the antioxidant activity also increased. Cell viability assay demonstrated that the Ndoped GQDs showed cell viability (70%) when the concentration reached 200 μg/mL for A549 and also MDA-MB-231, 150 μg/mL for NIH-3T3 cell lines at 24 h incubation. N-doped GQDs were coated with Eudragit RS 100 and EphA2-siRNA was loaded. As a result of the studies on these formulations, it was concluded that there may be significant effects on A549 cells. The microscopy results revealed that N-doped GQDs was quickly internalized into the cell. Our novel N-doped-GQDs with siRNA are candidate for in situ tumor suppression via DNA and mRNA breakage. Elsevier 2019-09 2019-05-21 /pmc/articles/PMC6733895/ /pubmed/31516327 http://dx.doi.org/10.1016/j.jsps.2019.05.006 Text en © 2019 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Şenel, Behiye Demir, Neslihan Büyükköroğlu, Gülay Yıldız, Mustafa Graphene quantum dots: Synthesis, characterization, cell viability, genotoxicity for biomedical applications |
title | Graphene quantum dots: Synthesis, characterization, cell viability, genotoxicity for biomedical applications |
title_full | Graphene quantum dots: Synthesis, characterization, cell viability, genotoxicity for biomedical applications |
title_fullStr | Graphene quantum dots: Synthesis, characterization, cell viability, genotoxicity for biomedical applications |
title_full_unstemmed | Graphene quantum dots: Synthesis, characterization, cell viability, genotoxicity for biomedical applications |
title_short | Graphene quantum dots: Synthesis, characterization, cell viability, genotoxicity for biomedical applications |
title_sort | graphene quantum dots: synthesis, characterization, cell viability, genotoxicity for biomedical applications |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6733895/ https://www.ncbi.nlm.nih.gov/pubmed/31516327 http://dx.doi.org/10.1016/j.jsps.2019.05.006 |
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