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Generation of genetically modified mice using SpCas9-NG engineered nuclease

Although genetically modified mice can be generated with high efficiency by using CRISPR/Cas9-mediated genome editing in mouse zygotes, only the loci with a protospacer-adjacent motif (PAM) sequence are targetable. The present study investigated the usability of engineered Streptococcus pyogenes Cas...

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Detalles Bibliográficos
Autores principales:	Fujii, Wataru, Ito, Haruka, Kanke, Takuya, Ikeda, Arisa, Sugiura, Koji, Naito, Kunihiko
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6733909/ https://www.ncbi.nlm.nih.gov/pubmed/31501500 http://dx.doi.org/10.1038/s41598-019-49394-5

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