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An all-in-one homogeneous DNA walking nanomachine and its application for intracellular analysis of miRNA
DNA walker is a powerful type of DNA nanomachine that can produce amplified signals during the "burnt-bridge"-like walking process. Despite their successful application in extracellular bioanalysis, the heterogeneity of the existing DNA walkers makes it difficult to guarantee the consisten...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6735375/ https://www.ncbi.nlm.nih.gov/pubmed/31534528 http://dx.doi.org/10.7150/thno.36081 |
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author | Hu, Muren Mao, Dongsheng Liu, Xiaohao Ren, Lingjie Zhou, Mengru Chen, Xiaoxia Zhu, Xiaoli |
author_facet | Hu, Muren Mao, Dongsheng Liu, Xiaohao Ren, Lingjie Zhou, Mengru Chen, Xiaoxia Zhu, Xiaoli |
author_sort | Hu, Muren |
collection | PubMed |
description | DNA walker is a powerful type of DNA nanomachine that can produce amplified signals during the "burnt-bridge"-like walking process. Despite their successful application in extracellular bioanalysis, the heterogeneity of the existing DNA walkers makes it difficult to guarantee the consistency of the results during the analysis of different cells. Methods: Here, an all-in-one homogeneous DNA walking nanomachine is reported that can be delivered into living cells for intracellular bioanalysis of miRNA without auxiliary materials. Results: This DNA walking nanomachine is constructed of gold nanoparticles on which two types of interrelated DNA tracks are assembled. The target miRNA, cancer-related miR-21, can be captured by one of the tracks (track 1) and then walk to the other track (track 2), releasing the hybrid of track 1 and track 2 from the nanoparticle to produce a signal. The walking process can proceed in a cyclic 1-2-1-2 manner and thereby produce amplified signals. Thus, sensitive imaging of the miRNA in situ can be achieved. Conclusion: Benefiting from the homogeneity of the detection system, the method can be applied for intracellular analysis without interference induced by the fluctuations of stimuli or accessorial contents. |
format | Online Article Text |
id | pubmed-6735375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-67353752019-09-18 An all-in-one homogeneous DNA walking nanomachine and its application for intracellular analysis of miRNA Hu, Muren Mao, Dongsheng Liu, Xiaohao Ren, Lingjie Zhou, Mengru Chen, Xiaoxia Zhu, Xiaoli Theranostics Research Paper DNA walker is a powerful type of DNA nanomachine that can produce amplified signals during the "burnt-bridge"-like walking process. Despite their successful application in extracellular bioanalysis, the heterogeneity of the existing DNA walkers makes it difficult to guarantee the consistency of the results during the analysis of different cells. Methods: Here, an all-in-one homogeneous DNA walking nanomachine is reported that can be delivered into living cells for intracellular bioanalysis of miRNA without auxiliary materials. Results: This DNA walking nanomachine is constructed of gold nanoparticles on which two types of interrelated DNA tracks are assembled. The target miRNA, cancer-related miR-21, can be captured by one of the tracks (track 1) and then walk to the other track (track 2), releasing the hybrid of track 1 and track 2 from the nanoparticle to produce a signal. The walking process can proceed in a cyclic 1-2-1-2 manner and thereby produce amplified signals. Thus, sensitive imaging of the miRNA in situ can be achieved. Conclusion: Benefiting from the homogeneity of the detection system, the method can be applied for intracellular analysis without interference induced by the fluctuations of stimuli or accessorial contents. Ivyspring International Publisher 2019-08-14 /pmc/articles/PMC6735375/ /pubmed/31534528 http://dx.doi.org/10.7150/thno.36081 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Hu, Muren Mao, Dongsheng Liu, Xiaohao Ren, Lingjie Zhou, Mengru Chen, Xiaoxia Zhu, Xiaoli An all-in-one homogeneous DNA walking nanomachine and its application for intracellular analysis of miRNA |
title | An all-in-one homogeneous DNA walking nanomachine and its application for intracellular analysis of miRNA |
title_full | An all-in-one homogeneous DNA walking nanomachine and its application for intracellular analysis of miRNA |
title_fullStr | An all-in-one homogeneous DNA walking nanomachine and its application for intracellular analysis of miRNA |
title_full_unstemmed | An all-in-one homogeneous DNA walking nanomachine and its application for intracellular analysis of miRNA |
title_short | An all-in-one homogeneous DNA walking nanomachine and its application for intracellular analysis of miRNA |
title_sort | all-in-one homogeneous dna walking nanomachine and its application for intracellular analysis of mirna |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6735375/ https://www.ncbi.nlm.nih.gov/pubmed/31534528 http://dx.doi.org/10.7150/thno.36081 |
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