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Hybrid Genome Assembly of a Neotropical Mutualistic Ant
The success of social insects is largely intertwined with their highly advanced chemical communication system that facilitates recognition and discrimination of species and nest-mates, recruitment, and division of labor. Hydrocarbons, which cover the cuticle of insects, not only serve as waterproofi...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6735702/ https://www.ncbi.nlm.nih.gov/pubmed/31329228 http://dx.doi.org/10.1093/gbe/evz159 |
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author | Hartke, Juliane Schell, Tilman Jongepier, Evelien Schmidt, Hanno Sprenger, Philipp P Paule, Juraj Bornberg-Bauer, Erich Schmitt, Thomas Menzel, Florian Pfenninger, Markus Feldmeyer, Barbara |
author_facet | Hartke, Juliane Schell, Tilman Jongepier, Evelien Schmidt, Hanno Sprenger, Philipp P Paule, Juraj Bornberg-Bauer, Erich Schmitt, Thomas Menzel, Florian Pfenninger, Markus Feldmeyer, Barbara |
author_sort | Hartke, Juliane |
collection | PubMed |
description | The success of social insects is largely intertwined with their highly advanced chemical communication system that facilitates recognition and discrimination of species and nest-mates, recruitment, and division of labor. Hydrocarbons, which cover the cuticle of insects, not only serve as waterproofing agents but also constitute a major component of this communication system. Two cryptic Crematogaster species, which share their nest with Camponotus ants, show striking diversity in their cuticular hydrocarbon (CHC) profile. This mutualistic system therefore offers a great opportunity to study the genetic basis of CHC divergence between sister species. As a basis for further genome-wide studies high-quality genomes are needed. Here, we present the annotated draft genome for Crematogaster levior A. By combining the three most commonly used sequencing techniques—Illumina, PacBio, and Oxford Nanopore—we constructed a high-quality de novo ant genome. We show that even low coverage of long reads can add significantly to overall genome contiguity. Annotation of desaturase and elongase genes, which play a role in CHC biosynthesis revealed one of the largest repertoires in ants and a higher number of desaturases in general than in other Hymenoptera. This may provide a mechanistic explanation for the high diversity observed in C. levior CHC profiles. |
format | Online Article Text |
id | pubmed-6735702 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-67357022019-09-16 Hybrid Genome Assembly of a Neotropical Mutualistic Ant Hartke, Juliane Schell, Tilman Jongepier, Evelien Schmidt, Hanno Sprenger, Philipp P Paule, Juraj Bornberg-Bauer, Erich Schmitt, Thomas Menzel, Florian Pfenninger, Markus Feldmeyer, Barbara Genome Biol Evol Genome Report The success of social insects is largely intertwined with their highly advanced chemical communication system that facilitates recognition and discrimination of species and nest-mates, recruitment, and division of labor. Hydrocarbons, which cover the cuticle of insects, not only serve as waterproofing agents but also constitute a major component of this communication system. Two cryptic Crematogaster species, which share their nest with Camponotus ants, show striking diversity in their cuticular hydrocarbon (CHC) profile. This mutualistic system therefore offers a great opportunity to study the genetic basis of CHC divergence between sister species. As a basis for further genome-wide studies high-quality genomes are needed. Here, we present the annotated draft genome for Crematogaster levior A. By combining the three most commonly used sequencing techniques—Illumina, PacBio, and Oxford Nanopore—we constructed a high-quality de novo ant genome. We show that even low coverage of long reads can add significantly to overall genome contiguity. Annotation of desaturase and elongase genes, which play a role in CHC biosynthesis revealed one of the largest repertoires in ants and a higher number of desaturases in general than in other Hymenoptera. This may provide a mechanistic explanation for the high diversity observed in C. levior CHC profiles. Oxford University Press 2019-07-22 /pmc/articles/PMC6735702/ /pubmed/31329228 http://dx.doi.org/10.1093/gbe/evz159 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Genome Report Hartke, Juliane Schell, Tilman Jongepier, Evelien Schmidt, Hanno Sprenger, Philipp P Paule, Juraj Bornberg-Bauer, Erich Schmitt, Thomas Menzel, Florian Pfenninger, Markus Feldmeyer, Barbara Hybrid Genome Assembly of a Neotropical Mutualistic Ant |
title | Hybrid Genome Assembly of a Neotropical Mutualistic Ant |
title_full | Hybrid Genome Assembly of a Neotropical Mutualistic Ant |
title_fullStr | Hybrid Genome Assembly of a Neotropical Mutualistic Ant |
title_full_unstemmed | Hybrid Genome Assembly of a Neotropical Mutualistic Ant |
title_short | Hybrid Genome Assembly of a Neotropical Mutualistic Ant |
title_sort | hybrid genome assembly of a neotropical mutualistic ant |
topic | Genome Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6735702/ https://www.ncbi.nlm.nih.gov/pubmed/31329228 http://dx.doi.org/10.1093/gbe/evz159 |
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