μLAS technology for DNA isolation coupled to Cas9-assisted targeting for sequencing and assembly of a 30 kb region in plant genome

Cas9-assisted targeting of DNA fragments in complex genomes is viewed as an essential strategy to obtain high-quality and continuous sequence data. However, the purity of target loci selected by pulsed-field gel electrophoresis (PFGE) has so far been insufficient to assemble the sequence in one cont...

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Detalles Bibliográficos
Autores principales: Milon, Nicolas, Chantry-Darmon, Céline, Satge, Carine, Fustier, Margaux-Alison, Cauet, Stephane, Moreau, Sandra, Callot, Caroline, Bellec, Arnaud, Gabrieli, Tslil, Saïas, Laure, Boutonnet, Audrey, Ginot, Frédéric, Bergès, Hélène, Bancaud, Aurélien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Genomics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6736094/
https://www.ncbi.nlm.nih.gov/pubmed/31505675
http://dx.doi.org/10.1093/nar/gkz632

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