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Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana
The intron-lariat spliceosome (ILS) complex is highly conserved among eukaryotes, and its disassembly marks the end of a canonical splicing cycle. In this study, we show that two conserved disassembly factors of the ILS complex, Increased Level of Polyploidy1-1D (ILP1) and NTC-Related protein 1 (NTR...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6736097/ https://www.ncbi.nlm.nih.gov/pubmed/31216029 http://dx.doi.org/10.1093/nar/gkz526 |
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author | Wang, Junli Chen, Susu Jiang, Ning Li, Ning Wang, Xiaoyan Li, Zhongpeng Li, Xu Liu, Hongtao Li, Lin Yang, Yu Ni, Ting Yu, Chaoyi Ma, Jinbiao Zheng, Binglian Ren, Guodong |
author_facet | Wang, Junli Chen, Susu Jiang, Ning Li, Ning Wang, Xiaoyan Li, Zhongpeng Li, Xu Liu, Hongtao Li, Lin Yang, Yu Ni, Ting Yu, Chaoyi Ma, Jinbiao Zheng, Binglian Ren, Guodong |
author_sort | Wang, Junli |
collection | PubMed |
description | The intron-lariat spliceosome (ILS) complex is highly conserved among eukaryotes, and its disassembly marks the end of a canonical splicing cycle. In this study, we show that two conserved disassembly factors of the ILS complex, Increased Level of Polyploidy1-1D (ILP1) and NTC-Related protein 1 (NTR1), positively regulate microRNA (miRNA) biogenesis by facilitating transcriptional elongation of MIRNA (MIR) genes in Arabidopsis thaliana. ILP1 and NTR1 formed a stable complex and co-regulated alternative splicing of more than a hundred genes across the Arabidopsis genome, including some primary transcripts of miRNAs (pri-miRNAs). Intriguingly, pri-miRNAs, regardless of having introns or not, were globally down-regulated when the ILP1 or NTR1 function was compromised. ILP1 and NTR1 interacted with core miRNA processing proteins Dicer-like 1 and Serrate, and were required for proper RNA polymerase II occupancy at elongated regions of MIR chromatin, without affecting either MIR promoter activity or pri-miRNA decay. Our results provide further insights into the regulatory role of spliceosomal machineries in the biogenesis of miRNAs. |
format | Online Article Text |
id | pubmed-6736097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-67360972019-09-16 Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana Wang, Junli Chen, Susu Jiang, Ning Li, Ning Wang, Xiaoyan Li, Zhongpeng Li, Xu Liu, Hongtao Li, Lin Yang, Yu Ni, Ting Yu, Chaoyi Ma, Jinbiao Zheng, Binglian Ren, Guodong Nucleic Acids Res Gene regulation, Chromatin and Epigenetics The intron-lariat spliceosome (ILS) complex is highly conserved among eukaryotes, and its disassembly marks the end of a canonical splicing cycle. In this study, we show that two conserved disassembly factors of the ILS complex, Increased Level of Polyploidy1-1D (ILP1) and NTC-Related protein 1 (NTR1), positively regulate microRNA (miRNA) biogenesis by facilitating transcriptional elongation of MIRNA (MIR) genes in Arabidopsis thaliana. ILP1 and NTR1 formed a stable complex and co-regulated alternative splicing of more than a hundred genes across the Arabidopsis genome, including some primary transcripts of miRNAs (pri-miRNAs). Intriguingly, pri-miRNAs, regardless of having introns or not, were globally down-regulated when the ILP1 or NTR1 function was compromised. ILP1 and NTR1 interacted with core miRNA processing proteins Dicer-like 1 and Serrate, and were required for proper RNA polymerase II occupancy at elongated regions of MIR chromatin, without affecting either MIR promoter activity or pri-miRNA decay. Our results provide further insights into the regulatory role of spliceosomal machineries in the biogenesis of miRNAs. Oxford University Press 2019-09-05 2019-06-19 /pmc/articles/PMC6736097/ /pubmed/31216029 http://dx.doi.org/10.1093/nar/gkz526 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Gene regulation, Chromatin and Epigenetics Wang, Junli Chen, Susu Jiang, Ning Li, Ning Wang, Xiaoyan Li, Zhongpeng Li, Xu Liu, Hongtao Li, Lin Yang, Yu Ni, Ting Yu, Chaoyi Ma, Jinbiao Zheng, Binglian Ren, Guodong Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana |
title | Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana |
title_full | Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana |
title_fullStr | Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana |
title_full_unstemmed | Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana |
title_short | Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana |
title_sort | spliceosome disassembly factors ilp1 and ntr1 promote mirna biogenesis in arabidopsis thaliana |
topic | Gene regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6736097/ https://www.ncbi.nlm.nih.gov/pubmed/31216029 http://dx.doi.org/10.1093/nar/gkz526 |
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