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Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana

The intron-lariat spliceosome (ILS) complex is highly conserved among eukaryotes, and its disassembly marks the end of a canonical splicing cycle. In this study, we show that two conserved disassembly factors of the ILS complex, Increased Level of Polyploidy1-1D (ILP1) and NTC-Related protein 1 (NTR...

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Autores principales: Wang, Junli, Chen, Susu, Jiang, Ning, Li, Ning, Wang, Xiaoyan, Li, Zhongpeng, Li, Xu, Liu, Hongtao, Li, Lin, Yang, Yu, Ni, Ting, Yu, Chaoyi, Ma, Jinbiao, Zheng, Binglian, Ren, Guodong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6736097/
https://www.ncbi.nlm.nih.gov/pubmed/31216029
http://dx.doi.org/10.1093/nar/gkz526
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author Wang, Junli
Chen, Susu
Jiang, Ning
Li, Ning
Wang, Xiaoyan
Li, Zhongpeng
Li, Xu
Liu, Hongtao
Li, Lin
Yang, Yu
Ni, Ting
Yu, Chaoyi
Ma, Jinbiao
Zheng, Binglian
Ren, Guodong
author_facet Wang, Junli
Chen, Susu
Jiang, Ning
Li, Ning
Wang, Xiaoyan
Li, Zhongpeng
Li, Xu
Liu, Hongtao
Li, Lin
Yang, Yu
Ni, Ting
Yu, Chaoyi
Ma, Jinbiao
Zheng, Binglian
Ren, Guodong
author_sort Wang, Junli
collection PubMed
description The intron-lariat spliceosome (ILS) complex is highly conserved among eukaryotes, and its disassembly marks the end of a canonical splicing cycle. In this study, we show that two conserved disassembly factors of the ILS complex, Increased Level of Polyploidy1-1D (ILP1) and NTC-Related protein 1 (NTR1), positively regulate microRNA (miRNA) biogenesis by facilitating transcriptional elongation of MIRNA (MIR) genes in Arabidopsis thaliana. ILP1 and NTR1 formed a stable complex and co-regulated alternative splicing of more than a hundred genes across the Arabidopsis genome, including some primary transcripts of miRNAs (pri-miRNAs). Intriguingly, pri-miRNAs, regardless of having introns or not, were globally down-regulated when the ILP1 or NTR1 function was compromised. ILP1 and NTR1 interacted with core miRNA processing proteins Dicer-like 1 and Serrate, and were required for proper RNA polymerase II occupancy at elongated regions of MIR chromatin, without affecting either MIR promoter activity or pri-miRNA decay. Our results provide further insights into the regulatory role of spliceosomal machineries in the biogenesis of miRNAs.
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spelling pubmed-67360972019-09-16 Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana Wang, Junli Chen, Susu Jiang, Ning Li, Ning Wang, Xiaoyan Li, Zhongpeng Li, Xu Liu, Hongtao Li, Lin Yang, Yu Ni, Ting Yu, Chaoyi Ma, Jinbiao Zheng, Binglian Ren, Guodong Nucleic Acids Res Gene regulation, Chromatin and Epigenetics The intron-lariat spliceosome (ILS) complex is highly conserved among eukaryotes, and its disassembly marks the end of a canonical splicing cycle. In this study, we show that two conserved disassembly factors of the ILS complex, Increased Level of Polyploidy1-1D (ILP1) and NTC-Related protein 1 (NTR1), positively regulate microRNA (miRNA) biogenesis by facilitating transcriptional elongation of MIRNA (MIR) genes in Arabidopsis thaliana. ILP1 and NTR1 formed a stable complex and co-regulated alternative splicing of more than a hundred genes across the Arabidopsis genome, including some primary transcripts of miRNAs (pri-miRNAs). Intriguingly, pri-miRNAs, regardless of having introns or not, were globally down-regulated when the ILP1 or NTR1 function was compromised. ILP1 and NTR1 interacted with core miRNA processing proteins Dicer-like 1 and Serrate, and were required for proper RNA polymerase II occupancy at elongated regions of MIR chromatin, without affecting either MIR promoter activity or pri-miRNA decay. Our results provide further insights into the regulatory role of spliceosomal machineries in the biogenesis of miRNAs. Oxford University Press 2019-09-05 2019-06-19 /pmc/articles/PMC6736097/ /pubmed/31216029 http://dx.doi.org/10.1093/nar/gkz526 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Gene regulation, Chromatin and Epigenetics
Wang, Junli
Chen, Susu
Jiang, Ning
Li, Ning
Wang, Xiaoyan
Li, Zhongpeng
Li, Xu
Liu, Hongtao
Li, Lin
Yang, Yu
Ni, Ting
Yu, Chaoyi
Ma, Jinbiao
Zheng, Binglian
Ren, Guodong
Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana
title Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana
title_full Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana
title_fullStr Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana
title_full_unstemmed Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana
title_short Spliceosome disassembly factors ILP1 and NTR1 promote miRNA biogenesis in Arabidopsis thaliana
title_sort spliceosome disassembly factors ilp1 and ntr1 promote mirna biogenesis in arabidopsis thaliana
topic Gene regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6736097/
https://www.ncbi.nlm.nih.gov/pubmed/31216029
http://dx.doi.org/10.1093/nar/gkz526
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