Engineering the expression system for Komagataella phaffii (Pichia pastoris): an attempt to develop a methanol-free expression system

The construction of a methanol-free expression system of Komagataella phaffii (Pichia pastoris) was attempted by engineering a strong methanol-inducible DAS1 promoter using Citrobacter braakii phytase production as a model case. Constitutive expression of KpTRM1, formerly PRM1—a positive transcripti...

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Detalles Bibliográficos
Autores principales: Takagi, Shinobu, Tsutsumi, Noriko, Terui, Yuji, Kong, XiangYu, Yurimoto, Hiroya, Sakai, Yasuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6736287/
https://www.ncbi.nlm.nih.gov/pubmed/31408151
http://dx.doi.org/10.1093/femsyr/foz059

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6736287/
https://www.ncbi.nlm.nih.gov/pubmed/31408151
http://dx.doi.org/10.1093/femsyr/foz059

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