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Effect of explant source, perlite nanoparticles and TiO(2)/perlite nanocomposites on phytochemical composition of metabolites in callus cultures of Hypericum perforatum

It appears that the biologically-synthesized nanoparticles (NPs) have potential to perform as effective elicitors for the production of valuable secondary metabolites in plants. Besides, it has been reported that the toxicity of the biologically-synthesized NP is not as much as that of the chemicall...

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Autores principales: Ebadollahi, R., Jafarirad, S., Kosari-Nasab, M., Mahjouri, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6736832/
https://www.ncbi.nlm.nih.gov/pubmed/31506546
http://dx.doi.org/10.1038/s41598-019-49504-3
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author Ebadollahi, R.
Jafarirad, S.
Kosari-Nasab, M.
Mahjouri, S.
author_facet Ebadollahi, R.
Jafarirad, S.
Kosari-Nasab, M.
Mahjouri, S.
author_sort Ebadollahi, R.
collection PubMed
description It appears that the biologically-synthesized nanoparticles (NPs) have potential to perform as effective elicitors for the production of valuable secondary metabolites in plants. Besides, it has been reported that the toxicity of the biologically-synthesized NP is not as much as that of the chemically-synthesized NPs. Therefore, it is necessary to test their advantages aspects. In this study, the physical synthesis of perlite NPs and biologically-synthesis of TiO(2)/perlite nanocomposites (NCs) were conducted. Subsequently, their effects and explant source influence on the growth characteristics and secondary metabolite profiles of Hypericum perforatum callus cultures were evaluated. According to the obtained results, morphology of the synthesized perlite NPs and TiO(2)/perlite NCs were mesoporous and spherical with sizes ranging about 14.51–23.34 and 15.50–24.61 nm, respectively. Addition of perlite NPs and TiO(2)/perlite NCs to the culture medium at the concentration range of 25–200 mg/L showed no adverse impacts on the growth characteristics of H. perforatum calli. According to the GC-MS analysis, the stress caused by perlite NPs and TiO(2)/perlite NCs led to an increase in the variety, amount and number of volatile compounds. The calli obtained from in vitro grown plants produced more volatile compounds relative to the calli obtained from field grown plants under the nanomaterial stress conditions. The production of hypericin and pseudohypericin were also determined in the callus cultures under desired nanomaterials elicitation. Accordingly, our results suggest that perlite NPs and TiO(2)/perlite NCs can possibly be considered as effective elicitors for the production of volatile compounds, hypericin, and pseudohypericin in callus cultures of H. perforatum.
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spelling pubmed-67368322019-09-20 Effect of explant source, perlite nanoparticles and TiO(2)/perlite nanocomposites on phytochemical composition of metabolites in callus cultures of Hypericum perforatum Ebadollahi, R. Jafarirad, S. Kosari-Nasab, M. Mahjouri, S. Sci Rep Article It appears that the biologically-synthesized nanoparticles (NPs) have potential to perform as effective elicitors for the production of valuable secondary metabolites in plants. Besides, it has been reported that the toxicity of the biologically-synthesized NP is not as much as that of the chemically-synthesized NPs. Therefore, it is necessary to test their advantages aspects. In this study, the physical synthesis of perlite NPs and biologically-synthesis of TiO(2)/perlite nanocomposites (NCs) were conducted. Subsequently, their effects and explant source influence on the growth characteristics and secondary metabolite profiles of Hypericum perforatum callus cultures were evaluated. According to the obtained results, morphology of the synthesized perlite NPs and TiO(2)/perlite NCs were mesoporous and spherical with sizes ranging about 14.51–23.34 and 15.50–24.61 nm, respectively. Addition of perlite NPs and TiO(2)/perlite NCs to the culture medium at the concentration range of 25–200 mg/L showed no adverse impacts on the growth characteristics of H. perforatum calli. According to the GC-MS analysis, the stress caused by perlite NPs and TiO(2)/perlite NCs led to an increase in the variety, amount and number of volatile compounds. The calli obtained from in vitro grown plants produced more volatile compounds relative to the calli obtained from field grown plants under the nanomaterial stress conditions. The production of hypericin and pseudohypericin were also determined in the callus cultures under desired nanomaterials elicitation. Accordingly, our results suggest that perlite NPs and TiO(2)/perlite NCs can possibly be considered as effective elicitors for the production of volatile compounds, hypericin, and pseudohypericin in callus cultures of H. perforatum. Nature Publishing Group UK 2019-09-10 /pmc/articles/PMC6736832/ /pubmed/31506546 http://dx.doi.org/10.1038/s41598-019-49504-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ebadollahi, R.
Jafarirad, S.
Kosari-Nasab, M.
Mahjouri, S.
Effect of explant source, perlite nanoparticles and TiO(2)/perlite nanocomposites on phytochemical composition of metabolites in callus cultures of Hypericum perforatum
title Effect of explant source, perlite nanoparticles and TiO(2)/perlite nanocomposites on phytochemical composition of metabolites in callus cultures of Hypericum perforatum
title_full Effect of explant source, perlite nanoparticles and TiO(2)/perlite nanocomposites on phytochemical composition of metabolites in callus cultures of Hypericum perforatum
title_fullStr Effect of explant source, perlite nanoparticles and TiO(2)/perlite nanocomposites on phytochemical composition of metabolites in callus cultures of Hypericum perforatum
title_full_unstemmed Effect of explant source, perlite nanoparticles and TiO(2)/perlite nanocomposites on phytochemical composition of metabolites in callus cultures of Hypericum perforatum
title_short Effect of explant source, perlite nanoparticles and TiO(2)/perlite nanocomposites on phytochemical composition of metabolites in callus cultures of Hypericum perforatum
title_sort effect of explant source, perlite nanoparticles and tio(2)/perlite nanocomposites on phytochemical composition of metabolites in callus cultures of hypericum perforatum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6736832/
https://www.ncbi.nlm.nih.gov/pubmed/31506546
http://dx.doi.org/10.1038/s41598-019-49504-3
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