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Genetic diversity, phylogenetic structure and development of core collections in Melilotus accessions from a Chinese gene bank

Melilotus is an important forage legume, with high values as feed and medicine, and widely used as green manure, honey plant, and wildlife habitat enhancer. The genetic diversity, structure and subdivision of this forage crop remain unclear, and plant genetic resources are the basis of biodiversity...

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Autores principales: Zhang, Hongxiang, Bai, Rong, Wu, Fan, Guo, Wenli, Yan, Zhuanzhuan, Yan, Qi, Zhang, Yufei, Ma, Jinxing, Zhang, Jiyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6736865/
https://www.ncbi.nlm.nih.gov/pubmed/31506537
http://dx.doi.org/10.1038/s41598-019-49355-y
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author Zhang, Hongxiang
Bai, Rong
Wu, Fan
Guo, Wenli
Yan, Zhuanzhuan
Yan, Qi
Zhang, Yufei
Ma, Jinxing
Zhang, Jiyu
author_facet Zhang, Hongxiang
Bai, Rong
Wu, Fan
Guo, Wenli
Yan, Zhuanzhuan
Yan, Qi
Zhang, Yufei
Ma, Jinxing
Zhang, Jiyu
author_sort Zhang, Hongxiang
collection PubMed
description Melilotus is an important forage legume, with high values as feed and medicine, and widely used as green manure, honey plant, and wildlife habitat enhancer. The genetic diversity, structure and subdivision of this forage crop remain unclear, and plant genetic resources are the basis of biodiversity and ecosystem diversity and have attracted increasing attention. In this study, the whole collection of 573 accessions from the National Gene Bank of Forage Germplasm (NGBFG, China) and 48 accessions from the National Plant Germplasm System (NPGS, USA) in genus Melilotus were measured with respect to five seed characters: seed length, width, width-to-length ratio, circumference and 100-seed weight. Shannon’ genetic diversity index (H’) and phenotypic differentiation (Pst) were calculated to better describe the genetic diversity. The ITS and matK sequences were used to construct phylogenetic trees and study the genetic relationships within genus Melilotu. Based on seed morphology and molecular marker data, we preliminarily developed core collections and the sampling rates of M. albus and M. officinalis were determined to be 15% and 25%, respectively. The results obtained here provide preliminary sorting and supplemental information for the Melilotus collections in NGBFG, China, and establish a reference for further genetic breeding and other related projects.
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spelling pubmed-67368652019-09-20 Genetic diversity, phylogenetic structure and development of core collections in Melilotus accessions from a Chinese gene bank Zhang, Hongxiang Bai, Rong Wu, Fan Guo, Wenli Yan, Zhuanzhuan Yan, Qi Zhang, Yufei Ma, Jinxing Zhang, Jiyu Sci Rep Article Melilotus is an important forage legume, with high values as feed and medicine, and widely used as green manure, honey plant, and wildlife habitat enhancer. The genetic diversity, structure and subdivision of this forage crop remain unclear, and plant genetic resources are the basis of biodiversity and ecosystem diversity and have attracted increasing attention. In this study, the whole collection of 573 accessions from the National Gene Bank of Forage Germplasm (NGBFG, China) and 48 accessions from the National Plant Germplasm System (NPGS, USA) in genus Melilotus were measured with respect to five seed characters: seed length, width, width-to-length ratio, circumference and 100-seed weight. Shannon’ genetic diversity index (H’) and phenotypic differentiation (Pst) were calculated to better describe the genetic diversity. The ITS and matK sequences were used to construct phylogenetic trees and study the genetic relationships within genus Melilotu. Based on seed morphology and molecular marker data, we preliminarily developed core collections and the sampling rates of M. albus and M. officinalis were determined to be 15% and 25%, respectively. The results obtained here provide preliminary sorting and supplemental information for the Melilotus collections in NGBFG, China, and establish a reference for further genetic breeding and other related projects. Nature Publishing Group UK 2019-09-10 /pmc/articles/PMC6736865/ /pubmed/31506537 http://dx.doi.org/10.1038/s41598-019-49355-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhang, Hongxiang
Bai, Rong
Wu, Fan
Guo, Wenli
Yan, Zhuanzhuan
Yan, Qi
Zhang, Yufei
Ma, Jinxing
Zhang, Jiyu
Genetic diversity, phylogenetic structure and development of core collections in Melilotus accessions from a Chinese gene bank
title Genetic diversity, phylogenetic structure and development of core collections in Melilotus accessions from a Chinese gene bank
title_full Genetic diversity, phylogenetic structure and development of core collections in Melilotus accessions from a Chinese gene bank
title_fullStr Genetic diversity, phylogenetic structure and development of core collections in Melilotus accessions from a Chinese gene bank
title_full_unstemmed Genetic diversity, phylogenetic structure and development of core collections in Melilotus accessions from a Chinese gene bank
title_short Genetic diversity, phylogenetic structure and development of core collections in Melilotus accessions from a Chinese gene bank
title_sort genetic diversity, phylogenetic structure and development of core collections in melilotus accessions from a chinese gene bank
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6736865/
https://www.ncbi.nlm.nih.gov/pubmed/31506537
http://dx.doi.org/10.1038/s41598-019-49355-y
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