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TRPA1 Ion Channel Determines Beneficial and Detrimental Effects of GYY4137 in Murine Serum-Transfer Arthritis

Modulation of nociception and inflammation by sulfide in rheumatoid arthritis and activation of transient receptor potential ankyrin 1 (TRPA1) ion channels by sulfide compounds are well documented. The present study aims to investigate TRPA1-mediated effects of sulfide donor GYY4137 in K/BxN serum-t...

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Autores principales: Bátai, István Z., Sár, Cecília Pápainé, Horváth, Ádám, Borbély, Éva, Bölcskei, Kata, Kemény, Ágnes, Sándor, Zoltán, Nemes, Balázs, Helyes, Zsuzsanna, Perkecz, Anikó, Mócsai, Attila, Pozsgai, Gábor, Pintér, Erika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6737045/
https://www.ncbi.nlm.nih.gov/pubmed/31551776
http://dx.doi.org/10.3389/fphar.2019.00964
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author Bátai, István Z.
Sár, Cecília Pápainé
Horváth, Ádám
Borbély, Éva
Bölcskei, Kata
Kemény, Ágnes
Sándor, Zoltán
Nemes, Balázs
Helyes, Zsuzsanna
Perkecz, Anikó
Mócsai, Attila
Pozsgai, Gábor
Pintér, Erika
author_facet Bátai, István Z.
Sár, Cecília Pápainé
Horváth, Ádám
Borbély, Éva
Bölcskei, Kata
Kemény, Ágnes
Sándor, Zoltán
Nemes, Balázs
Helyes, Zsuzsanna
Perkecz, Anikó
Mócsai, Attila
Pozsgai, Gábor
Pintér, Erika
author_sort Bátai, István Z.
collection PubMed
description Modulation of nociception and inflammation by sulfide in rheumatoid arthritis and activation of transient receptor potential ankyrin 1 (TRPA1) ion channels by sulfide compounds are well documented. The present study aims to investigate TRPA1-mediated effects of sulfide donor GYY4137 in K/BxN serum-transfer arthritis, a rodent model of rheumatoid arthritis. TRPA1 and somatostatin sst4 receptor wild-type (WT) and knockout mice underwent K/BxN serum transfer and were treated daily with GYY4137. Functional and biochemical signs of inflammation were recorded, together with histological characterization. These included detection of hind paw mechanical hyperalgesia by dynamic plantar esthesiometry, hind paw volume by plethysmometry, and upside-down hanging time to failure. Hind paw erythema, edema, and passive movement range of tibiotarsal joints were scored. Somatostatin release from sensory nerve endings of TRPA1 wild-type and knockout mice in response to polysulfide was detected by radioimmunoassay. Polysulfide formation from GYY4137 was uncovered by cold cyanolysis. GYY4137 aggravated mechanical hyperalgesia in TRPA1 knockout mice but ameliorated it in wild-type ones. Arthritis score was lowered by GYY4137 in TRPA1 wild-type animals. Increased myeloperoxidase activity, plasma extravasation, and subcutaneous MIP-2 levels of hind paws were detected in TRPA1 knockout mice upon GYY4137 treatment. Genetic lack of sst4 receptors did not alter mechanical hyperalgesia, edema formation, hanging performance, arthritis score, plasma extravasation, or myeloperoxidase activity. TRPA1 WT animals exhibited smaller cartilage destruction upon GYY4137 administration. Sodium polysulfide caused TRPA1-dependent somatostatin release from murine nerve endings. Sulfide released from GYY4137 is readily converted into polysulfide by hypochlorite. Polysulfide potently activates human TRPA1 receptors expressed in Chinese hamster ovary (CHO) cells. According to our data, the protective effect of GYY4137 is mediated by TRPA1, while detrimental actions are independent of the ion channel in the K/BxN serum-transfer arthritis model in mice. At acidic pH in inflamed tissue sulfide is released from GYY4137 and reacts with neutrophil-derived hypochlorite. Resulting polysulfide might be responsible for TRPA1-mediated antinociceptive and anti-inflammatory as well as TRPA1-independent pro-inflammatory effects.
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spelling pubmed-67370452019-09-24 TRPA1 Ion Channel Determines Beneficial and Detrimental Effects of GYY4137 in Murine Serum-Transfer Arthritis Bátai, István Z. Sár, Cecília Pápainé Horváth, Ádám Borbély, Éva Bölcskei, Kata Kemény, Ágnes Sándor, Zoltán Nemes, Balázs Helyes, Zsuzsanna Perkecz, Anikó Mócsai, Attila Pozsgai, Gábor Pintér, Erika Front Pharmacol Pharmacology Modulation of nociception and inflammation by sulfide in rheumatoid arthritis and activation of transient receptor potential ankyrin 1 (TRPA1) ion channels by sulfide compounds are well documented. The present study aims to investigate TRPA1-mediated effects of sulfide donor GYY4137 in K/BxN serum-transfer arthritis, a rodent model of rheumatoid arthritis. TRPA1 and somatostatin sst4 receptor wild-type (WT) and knockout mice underwent K/BxN serum transfer and were treated daily with GYY4137. Functional and biochemical signs of inflammation were recorded, together with histological characterization. These included detection of hind paw mechanical hyperalgesia by dynamic plantar esthesiometry, hind paw volume by plethysmometry, and upside-down hanging time to failure. Hind paw erythema, edema, and passive movement range of tibiotarsal joints were scored. Somatostatin release from sensory nerve endings of TRPA1 wild-type and knockout mice in response to polysulfide was detected by radioimmunoassay. Polysulfide formation from GYY4137 was uncovered by cold cyanolysis. GYY4137 aggravated mechanical hyperalgesia in TRPA1 knockout mice but ameliorated it in wild-type ones. Arthritis score was lowered by GYY4137 in TRPA1 wild-type animals. Increased myeloperoxidase activity, plasma extravasation, and subcutaneous MIP-2 levels of hind paws were detected in TRPA1 knockout mice upon GYY4137 treatment. Genetic lack of sst4 receptors did not alter mechanical hyperalgesia, edema formation, hanging performance, arthritis score, plasma extravasation, or myeloperoxidase activity. TRPA1 WT animals exhibited smaller cartilage destruction upon GYY4137 administration. Sodium polysulfide caused TRPA1-dependent somatostatin release from murine nerve endings. Sulfide released from GYY4137 is readily converted into polysulfide by hypochlorite. Polysulfide potently activates human TRPA1 receptors expressed in Chinese hamster ovary (CHO) cells. According to our data, the protective effect of GYY4137 is mediated by TRPA1, while detrimental actions are independent of the ion channel in the K/BxN serum-transfer arthritis model in mice. At acidic pH in inflamed tissue sulfide is released from GYY4137 and reacts with neutrophil-derived hypochlorite. Resulting polysulfide might be responsible for TRPA1-mediated antinociceptive and anti-inflammatory as well as TRPA1-independent pro-inflammatory effects. Frontiers Media S.A. 2019-09-04 /pmc/articles/PMC6737045/ /pubmed/31551776 http://dx.doi.org/10.3389/fphar.2019.00964 Text en Copyright © 2019 Bátai, Sár, Horváth, Borbély, Bölcskei, Kemény, Sándor, Nemes, Helyes, Perkecz, Mócsai, Pozsgai and Pintér http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Bátai, István Z.
Sár, Cecília Pápainé
Horváth, Ádám
Borbély, Éva
Bölcskei, Kata
Kemény, Ágnes
Sándor, Zoltán
Nemes, Balázs
Helyes, Zsuzsanna
Perkecz, Anikó
Mócsai, Attila
Pozsgai, Gábor
Pintér, Erika
TRPA1 Ion Channel Determines Beneficial and Detrimental Effects of GYY4137 in Murine Serum-Transfer Arthritis
title TRPA1 Ion Channel Determines Beneficial and Detrimental Effects of GYY4137 in Murine Serum-Transfer Arthritis
title_full TRPA1 Ion Channel Determines Beneficial and Detrimental Effects of GYY4137 in Murine Serum-Transfer Arthritis
title_fullStr TRPA1 Ion Channel Determines Beneficial and Detrimental Effects of GYY4137 in Murine Serum-Transfer Arthritis
title_full_unstemmed TRPA1 Ion Channel Determines Beneficial and Detrimental Effects of GYY4137 in Murine Serum-Transfer Arthritis
title_short TRPA1 Ion Channel Determines Beneficial and Detrimental Effects of GYY4137 in Murine Serum-Transfer Arthritis
title_sort trpa1 ion channel determines beneficial and detrimental effects of gyy4137 in murine serum-transfer arthritis
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6737045/
https://www.ncbi.nlm.nih.gov/pubmed/31551776
http://dx.doi.org/10.3389/fphar.2019.00964
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