First Molecular Survey on Anaplasma phagocytophilum Revealed High Prevalence in Rural Dogs from Khuzestan Province, Iran

BACKGROUND: Anaplasmosis due to Anaplasma phagocytophilum is an important tick-borne zoonotic disease, which affects dogs, horses, cattle and human as well. This study aimed to probe the existence of this organism by means of molecular biology techniques for the first time in rural dogs of Khuzestan...

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Autores principales: HAMIDINEJAT, Hossein, BAHRAMI, Somayeh, MOSALANEJAD, Bahman, PAHLAVAN, Sharareh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2019
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6737366/
https://www.ncbi.nlm.nih.gov/pubmed/31543918
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author HAMIDINEJAT, Hossein
BAHRAMI, Somayeh
MOSALANEJAD, Bahman
PAHLAVAN, Sharareh
author_facet HAMIDINEJAT, Hossein
BAHRAMI, Somayeh
MOSALANEJAD, Bahman
PAHLAVAN, Sharareh
author_sort HAMIDINEJAT, Hossein
collection PubMed
description BACKGROUND: Anaplasmosis due to Anaplasma phagocytophilum is an important tick-borne zoonotic disease, which affects dogs, horses, cattle and human as well. This study aimed to probe the existence of this organism by means of molecular biology techniques for the first time in rural dogs of Khuzestan province, Southwestern Iran. METHODS: During Sep 2014 to Apr 2015 blood samples of 103 apparently healthy rural dogs (60 males) were collected for A. phagocytophilum detection by light microscopical examination of Giemsa stained slides and Nested PCR on a fragment of 16S rRNA gene. RESULTS: From the examined slides, 11.65% were positive for A. morulae while 57.28% of infection was revealed by Nested PCR method. There was no statistical difference between ages and sexes of dogs and infection in molecular survey of A. phagocytophilum. CONCLUSION: Molecular prevalence of A. phagocytophilum was noticeably high. It may cause the incidence of disease in human population.
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spelling pubmed-67373662019-09-22 First Molecular Survey on Anaplasma phagocytophilum Revealed High Prevalence in Rural Dogs from Khuzestan Province, Iran HAMIDINEJAT, Hossein BAHRAMI, Somayeh MOSALANEJAD, Bahman PAHLAVAN, Sharareh Iran J Parasitol Original Article BACKGROUND: Anaplasmosis due to Anaplasma phagocytophilum is an important tick-borne zoonotic disease, which affects dogs, horses, cattle and human as well. This study aimed to probe the existence of this organism by means of molecular biology techniques for the first time in rural dogs of Khuzestan province, Southwestern Iran. METHODS: During Sep 2014 to Apr 2015 blood samples of 103 apparently healthy rural dogs (60 males) were collected for A. phagocytophilum detection by light microscopical examination of Giemsa stained slides and Nested PCR on a fragment of 16S rRNA gene. RESULTS: From the examined slides, 11.65% were positive for A. morulae while 57.28% of infection was revealed by Nested PCR method. There was no statistical difference between ages and sexes of dogs and infection in molecular survey of A. phagocytophilum. CONCLUSION: Molecular prevalence of A. phagocytophilum was noticeably high. It may cause the incidence of disease in human population. Tehran University of Medical Sciences 2019 /pmc/articles/PMC6737366/ /pubmed/31543918 Text en Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
HAMIDINEJAT, Hossein
BAHRAMI, Somayeh
MOSALANEJAD, Bahman
PAHLAVAN, Sharareh
First Molecular Survey on Anaplasma phagocytophilum Revealed High Prevalence in Rural Dogs from Khuzestan Province, Iran
title First Molecular Survey on Anaplasma phagocytophilum Revealed High Prevalence in Rural Dogs from Khuzestan Province, Iran
title_full First Molecular Survey on Anaplasma phagocytophilum Revealed High Prevalence in Rural Dogs from Khuzestan Province, Iran
title_fullStr First Molecular Survey on Anaplasma phagocytophilum Revealed High Prevalence in Rural Dogs from Khuzestan Province, Iran
title_full_unstemmed First Molecular Survey on Anaplasma phagocytophilum Revealed High Prevalence in Rural Dogs from Khuzestan Province, Iran
title_short First Molecular Survey on Anaplasma phagocytophilum Revealed High Prevalence in Rural Dogs from Khuzestan Province, Iran
title_sort first molecular survey on anaplasma phagocytophilum revealed high prevalence in rural dogs from khuzestan province, iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6737366/
https://www.ncbi.nlm.nih.gov/pubmed/31543918
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