Expression of antioxidant genes in broiler chickens fed nettle (Urtica dioica) and its link with pulmonary hypertension

Nettle (Urtica dioica) contains a wide range of chemical constituents that confer a strong antioxidant capacity to the plant. The present study was to investigate the antioxidant gene expression and pulmonary hypertensive responses of broiler chickens to U. dioica. A total of 240 one-d-old broilers (Ross 308) were randomly assigned to 4 dietary levels of U. dioica (0, 0.5%, 1% and 1.5%). Birds were reared for 6 wk in a high altitude region (2,100 m). The results showed a significant relative overexpression (target gene/β-actin as the arbitrary unit) of catalase (CAT) and superoxide dismutase 1 (SOD1) in the liver and lung of the chickens fed U. dioica. Lipid peroxidation was significantly suppressed, as reflected in reduced circulatory concentrations of malondialdehyde (MDA) in the birds fed U. dioica. These birds also had significantly (P < 0.05) higher serum nitric oxide (NO) concentrations than those in the control group. Feeding U. dioica at 1% and 1.5% also attenuated the right ventricular hypertrophy (reflected in the lower right to total ventricular weight ratio), which was associated with a significant lower rate of mortality from pulmonary hypertension syndrome. Feeding U. dioica led to an upregulation of hepatic and pulmonary antioxidant genes.