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Emergence of IncX3 Plasmid-Harboring bla(NDM–5) Dominated by Escherichia coli ST48 in a Goose Farm in Jiangsu, China

Twelve carbapenem-resistant Escherichia coli strains were obtained from goose farms in Jiangsu, China. These isolates were resistant to multiple antimicrobials, and positive for the bla(NDM–)(5). The carbapenem-resistance of all strains mediated by bla(NDM–)(5) were successfully conjugated to E. col...

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Autores principales: Liu, Ziyi, Xiao, Xia, Li, Yan, Liu, Yuan, Li, Ruichao, Wang, Zhiqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6737504/
https://www.ncbi.nlm.nih.gov/pubmed/31551956
http://dx.doi.org/10.3389/fmicb.2019.02002
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author Liu, Ziyi
Xiao, Xia
Li, Yan
Liu, Yuan
Li, Ruichao
Wang, Zhiqiang
author_facet Liu, Ziyi
Xiao, Xia
Li, Yan
Liu, Yuan
Li, Ruichao
Wang, Zhiqiang
author_sort Liu, Ziyi
collection PubMed
description Twelve carbapenem-resistant Escherichia coli strains were obtained from goose farms in Jiangsu, China. These isolates were resistant to multiple antimicrobials, and positive for the bla(NDM–)(5). The carbapenem-resistance of all strains mediated by bla(NDM–)(5) were successfully conjugated to E. coli J53. S1-PFGE and WGS results showed bla(NDM–)(5) was located on IncX3 conjugative plasmids with a size of ca. 46 kb. All bla(NDM–)(5)-bearing IncX3 plasmids shared the same genetic context almost identical to pNDM_MGR194-bla(NDM–)(5) and pNDM-QD28-bla(NDM–)(5) reported in India and China, respectively. The twelve strains belonged to three STs, in which the dominant type of E. coli isolated from breeding goose farm carrying bla(NDM–)(5) was ST48. The emergence of bla(NDM–)(5)-bearing strains in goose farms and the clonal transmission of E. coli within the breeding goose farm highlighted the potential reservoir of carbapenemase genes in waterfowl farming system, which may further contaminate environments and pose a threat to public health. Comprehensive surveillance of carbapenem-resistant bacteria in goose farms warrants further study to evaluate the underlying risks.
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spelling pubmed-67375042019-09-24 Emergence of IncX3 Plasmid-Harboring bla(NDM–5) Dominated by Escherichia coli ST48 in a Goose Farm in Jiangsu, China Liu, Ziyi Xiao, Xia Li, Yan Liu, Yuan Li, Ruichao Wang, Zhiqiang Front Microbiol Microbiology Twelve carbapenem-resistant Escherichia coli strains were obtained from goose farms in Jiangsu, China. These isolates were resistant to multiple antimicrobials, and positive for the bla(NDM–)(5). The carbapenem-resistance of all strains mediated by bla(NDM–)(5) were successfully conjugated to E. coli J53. S1-PFGE and WGS results showed bla(NDM–)(5) was located on IncX3 conjugative plasmids with a size of ca. 46 kb. All bla(NDM–)(5)-bearing IncX3 plasmids shared the same genetic context almost identical to pNDM_MGR194-bla(NDM–)(5) and pNDM-QD28-bla(NDM–)(5) reported in India and China, respectively. The twelve strains belonged to three STs, in which the dominant type of E. coli isolated from breeding goose farm carrying bla(NDM–)(5) was ST48. The emergence of bla(NDM–)(5)-bearing strains in goose farms and the clonal transmission of E. coli within the breeding goose farm highlighted the potential reservoir of carbapenemase genes in waterfowl farming system, which may further contaminate environments and pose a threat to public health. Comprehensive surveillance of carbapenem-resistant bacteria in goose farms warrants further study to evaluate the underlying risks. Frontiers Media S.A. 2019-09-04 /pmc/articles/PMC6737504/ /pubmed/31551956 http://dx.doi.org/10.3389/fmicb.2019.02002 Text en Copyright © 2019 Liu, Xiao, Li, Liu, Li and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Ziyi
Xiao, Xia
Li, Yan
Liu, Yuan
Li, Ruichao
Wang, Zhiqiang
Emergence of IncX3 Plasmid-Harboring bla(NDM–5) Dominated by Escherichia coli ST48 in a Goose Farm in Jiangsu, China
title Emergence of IncX3 Plasmid-Harboring bla(NDM–5) Dominated by Escherichia coli ST48 in a Goose Farm in Jiangsu, China
title_full Emergence of IncX3 Plasmid-Harboring bla(NDM–5) Dominated by Escherichia coli ST48 in a Goose Farm in Jiangsu, China
title_fullStr Emergence of IncX3 Plasmid-Harboring bla(NDM–5) Dominated by Escherichia coli ST48 in a Goose Farm in Jiangsu, China
title_full_unstemmed Emergence of IncX3 Plasmid-Harboring bla(NDM–5) Dominated by Escherichia coli ST48 in a Goose Farm in Jiangsu, China
title_short Emergence of IncX3 Plasmid-Harboring bla(NDM–5) Dominated by Escherichia coli ST48 in a Goose Farm in Jiangsu, China
title_sort emergence of incx3 plasmid-harboring bla(ndm–5) dominated by escherichia coli st48 in a goose farm in jiangsu, china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6737504/
https://www.ncbi.nlm.nih.gov/pubmed/31551956
http://dx.doi.org/10.3389/fmicb.2019.02002
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